Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LJX92_RS25190 Genome accession   NZ_CP086206
Coordinates   5633485..5634519 (-) Length   344 a.a.
NCBI ID   WP_032878931.1    Uniprot ID   -
Organism   Pseudomonas sp. HN8-3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5628485..5639519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJX92_RS25160 (LJX92_25190) ruvX 5628565..5629002 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  LJX92_RS25165 (LJX92_25195) pyrR 5629135..5629641 (+) 507 WP_005792260.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LJX92_RS25170 (LJX92_25200) - 5629666..5630670 (+) 1005 WP_032878936.1 aspartate carbamoyltransferase catalytic subunit -
  LJX92_RS25175 (LJX92_25205) - 5630667..5631938 (+) 1272 WP_228309078.1 dihydroorotase -
  LJX92_RS25180 (LJX92_25210) - 5632021..5632449 (-) 429 WP_032878933.1 NINE protein -
  LJX92_RS25185 (LJX92_25215) - 5632699..5633337 (+) 639 WP_005792265.1 C40 family peptidase -
  LJX92_RS25190 (LJX92_25220) pilT 5633485..5634519 (-) 1035 WP_032878931.1 type IV pilus twitching motility protein PilT Machinery gene
  LJX92_RS25195 (LJX92_25225) - 5634577..5635272 (+) 696 WP_228309079.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJX92_RS25200 (LJX92_25230) proC 5635298..5636116 (+) 819 WP_228309080.1 pyrroline-5-carboxylate reductase -
  LJX92_RS25205 (LJX92_25235) - 5636142..5636729 (+) 588 WP_003195087.1 YggT family protein -
  LJX92_RS25210 (LJX92_25240) metX 5636951..5638090 (+) 1140 WP_005792271.1 homoserine O-succinyltransferase MetX -
  LJX92_RS25215 (LJX92_25245) metW 5638098..5638718 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  LJX92_RS25220 (LJX92_25250) - 5638740..5639174 (+) 435 WP_228309081.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37443.36 Da        Isoelectric Point: 7.2665

>NTDB_id=623685 LJX92_RS25190 WP_032878931.1 5633485..5634519(-) (pilT) [Pseudomonas sp. HN8-3]
MDITQLLTASVSRGACDLHLSAGLAPMVRVDGEVWPMDEPVLSAAQLVDLVSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQNRGIGAVFRAIPTEVQSLEDLGLGEVFQRMAQFPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKLALITQRQVHRDTHSFCVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLVSREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=623685 LJX92_RS25190 WP_032878931.1 5633485..5634519(-) (pilT) [Pseudomonas sp. HN8-3]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGTGGCGCCTGTGACCTGCATTTGTCGGCCGGCTTGGCGCCGAT
GGTGCGAGTCGATGGCGAGGTCTGGCCGATGGATGAGCCGGTGCTGAGCGCAGCGCAACTGGTCGATCTGGTGAGCCCTT
TGTTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCGGGAGTCGCACGC
TTTCGGGCGAACGTGTTCCGCCAGAATCGCGGTATAGGCGCGGTGTTTCGTGCCATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTTGGGTTGGGCGAGGTATTCCAGCGTATGGCTCAGTTCCCCCGTGGCCTGGTGCTGGTCACCGGGCCTACGGGTT
CGGGCAAGTCCACGACGCTGGCGGCGATGATCGATTATCTGAACCGGCACCGGCGCCAGCACATCCTCACGCTTGAAGAC
CCCATCGAATTTATCCACAGGCCGAAACTGGCCCTGATCACCCAACGGCAGGTGCACCGCGACACCCACAGCTTCTGCGT
GGCCCTGCGCTCGGCCCTGAGGGAAGACCCTGATGTGATCCTGGTAGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CCTTGACGGCAGCTGAGACCGGGCATCTGGTGTTTGGCACCCTGCACACTTCATCGGCTGCAAAGACTGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCTCAGGT
ACTGGTGAAGAAGGTCGGCGGCGGCCGTGTGGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTTTCGGCAATTCAGACCGGTGGCGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGCTGGTCAGCCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCTGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

67.742

99.128

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.095

97.965

0.628
  pilT Vibrio cholerae strain A1552

64.095

97.965

0.628
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Neisseria gonorrhoeae MS11

61.176

98.837

0.605
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Acinetobacter baylyi ADP1

37.572

100

0.378