Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LKW30_RS06515 Genome accession   NZ_CP086060
Coordinates   1404059..1405102 (-) Length   347 a.a.
NCBI ID   WP_011793812.1    Uniprot ID   A1TJV3
Organism   Paracidovorax citrulli strain HPP21-9-4B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1399059..1410102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LKW30_RS06485 - 1399307..1399711 (+) 405 WP_046060366.1 YraN family protein -
  LKW30_RS06490 - 1399783..1400385 (+) 603 WP_046060367.1 SIS domain-containing protein -
  LKW30_RS06495 - 1400382..1401038 (+) 657 WP_017438694.1 BON domain-containing protein -
  LKW30_RS06500 - 1401145..1402083 (-) 939 WP_011793808.1 NAD(P)-dependent oxidoreductase -
  LKW30_RS06505 pilU 1402167..1403303 (-) 1137 WP_011793810.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LKW30_RS06510 - 1403332..1403970 (-) 639 WP_011793811.1 cyclic nucleotide-binding domain-containing protein -
  LKW30_RS06515 pilT 1404059..1405102 (-) 1044 WP_011793812.1 type IV pilus twitching motility protein PilT Machinery gene
  LKW30_RS06520 - 1405144..1405869 (+) 726 WP_048857568.1 YggS family pyridoxal phosphate-dependent enzyme -
  LKW30_RS06525 ltaE 1405902..1406966 (-) 1065 WP_017439707.1 low-specificity L-threonine aldolase -
  LKW30_RS06530 - 1407197..1408336 (+) 1140 WP_046060368.1 aminotransferase class V-fold PLP-dependent enzyme -
  LKW30_RS06535 - 1408550..1409974 (+) 1425 WP_116212437.1 DUF445 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38132.93 Da        Isoelectric Point: 6.8354

>NTDB_id=622128 LKW30_RS06515 WP_011793812.1 1404059..1405102(-) (pilT) [Paracidovorax citrulli strain HPP21-9-4B]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKVYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSSSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=622128 LKW30_RS06515 WP_011793812.1 1404059..1405102(-) (pilT) [Paracidovorax citrulli strain HPP21-9-4B]
GTGGACATCACCCAACTGCTCGCCTTCAGCGTGAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGCCTGCCTCCGAT
GATCCGCGTGCATGGCGACGTGCGCCGCATCAACGTCGATGCCCTCGACCACAAGACCGTGCACGCCATGGTGTACGACA
TCATGAGCGACGCCCAGCGCAAGGTGTACGAGGAGTTCCTGGAAGTGGACTTCTCCTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGGACCATCCCGAGCAAGATCCTCACGCTCGA
GCAGCTCAACGCCCCGCGCATCTTCGCCGACCTTGCCCTCAAGCCGCGCGGCCTCGTGCTCGTCACCGGCCCCACCGGCT
CGGGCAAGTCCACCACGCTCGCGGCCATGGTGAACCACCTCAACGAGACCGAATACGGGCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGGCCGATGACGCTGTCCTTCGCCGC
CGCGCTGAAATCCGCGCTGCGCGAAGACCCGGACGCCATCCTCGTGGGCGAAATGCGCGACCTCGAAACCATCCGCCTGG
CCATGACCGCCGCCGAGACGGGCCACCTCGTGTTCGGCACCCTGCACACTTCCAGCGCGGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTGATCTCCCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCACGAGATCATGCTGGGCACGAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAGCAGCGTCGGCATGCAGACGCTGGACCAGAAC
CTCACCGACCTCGTGCGGCGCAACATCATCAGCCCCGCGGAAGCCCGCGGCAAGGCCAAGATTCCCGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1TJV3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.155

98.271

0.66
  pilT Vibrio cholerae strain A1552

67.155

98.271

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401