Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LK421_RS02030 Genome accession   NZ_CP085930
Coordinates   474360..475421 (-) Length   353 a.a.
NCBI ID   WP_004848860.1    Uniprot ID   -
Organism   Coprococcus eutactus ATCC 27759 strain FDAARGOS_1581     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469360..480421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK421_RS02010 (LK421_02010) - 469360..470112 (-) 753 WP_004848871.1 hypothetical protein -
  LK421_RS02015 (LK421_02015) pilM 470125..471744 (-) 1620 WP_004848869.1 type IV pilus assembly protein PilM -
  LK421_RS02020 (LK421_02020) - 472266..472718 (-) 453 WP_004848864.1 type II secretion system protein -
  LK421_RS02025 (LK421_02025) - 472922..474133 (-) 1212 WP_004848862.1 type II secretion system F family protein -
  LK421_RS02030 (LK421_02030) pilT 474360..475421 (-) 1062 WP_004848860.1 type IV pilus twitching motility protein PilT Machinery gene
  LK421_RS02035 (LK421_02035) - 475512..477254 (-) 1743 WP_004848858.1 GspE/PulE family protein -
  LK421_RS02040 (LK421_02040) sigK 477562..478185 (+) 624 WP_004848854.1 RNA polymerase sporulation sigma factor SigK -
  LK421_RS02045 (LK421_02045) - 478250..479482 (-) 1233 WP_004848852.1 peptidase U32 family protein -
  LK421_RS02050 (LK421_02050) - 479507..480148 (-) 642 WP_004848851.1 O-methyltransferase -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38956.52 Da        Isoelectric Point: 6.2437

>NTDB_id=621335 LK421_RS02030 WP_004848860.1 474360..475421(-) (pilT) [Coprococcus eutactus ATCC 27759 strain FDAARGOS_1581]
MLDMKELLAFSVEERASDVHIAVGIPPKLRINGKLTEVEVPPLTPADAAEAIGSTMKDRHKAILQDRGEVDFSFDSPETG
RFRVNVFMDRGNMAAAYRRVETQIPRPDQLGIPREVVELYKRKRGLVLVTGPTGSGKSTTLASIINKANENLTDHIITLE
DPIEYIHHHNKAIVNQREVGMDTLSYANALRAALREDPDIILVGEMRDLETISTAITAAETGHLVFSTLHTIGAASTIDR
IIDIFPTNQQQQVRVQLAMVLEGVISQSLLPIASGNGRVAAFEVMLASPAIRSLIREAKTHQIQSTIQTSRSLGMVTMDD
SLYELYASGTVTRETAMTYAQDQQYLRKKINGQ

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=621335 LK421_RS02030 WP_004848860.1 474360..475421(-) (pilT) [Coprococcus eutactus ATCC 27759 strain FDAARGOS_1581]
ATGCTGGATATGAAAGAACTGCTTGCTTTCTCCGTTGAGGAGAGAGCTTCAGATGTTCACATAGCTGTAGGAATACCACC
TAAGCTTAGGATCAACGGAAAACTCACGGAGGTTGAGGTTCCGCCTCTTACACCGGCTGACGCAGCCGAGGCAATAGGTT
CAACCATGAAGGACAGACATAAGGCGATACTTCAGGACAGAGGAGAGGTCGATTTCTCATTCGACTCACCGGAGACCGGA
CGTTTCCGTGTGAATGTATTCATGGACAGAGGAAACATGGCTGCCGCTTACAGACGAGTTGAGACGCAGATACCAAGACC
AGATCAGTTAGGTATACCTAGAGAGGTTGTTGAACTGTACAAGAGAAAGAGAGGACTTGTCCTTGTAACCGGACCTACCG
GATCAGGAAAGTCGACCACACTTGCTTCTATTATTAATAAGGCGAATGAAAACCTCACGGATCACATCATAACACTTGAG
GATCCTATAGAGTACATACATCACCACAACAAGGCGATAGTCAACCAGCGAGAGGTTGGTATGGATACCCTCAGCTATGC
GAATGCACTTCGTGCAGCACTTCGTGAGGATCCTGATATAATCCTCGTAGGAGAGATGCGAGATCTTGAGACTATCTCAA
CAGCTATAACAGCTGCCGAGACCGGACACCTGGTATTCTCAACACTGCATACGATTGGAGCGGCCAGTACCATCGACCGT
ATCATAGATATCTTCCCTACGAACCAGCAGCAGCAGGTAAGAGTTCAGCTTGCCATGGTTCTTGAAGGTGTCATATCGCA
GTCATTGCTTCCTATAGCAAGTGGCAACGGACGAGTAGCAGCATTCGAGGTCATGCTTGCATCACCTGCTATACGAAGTC
TGATACGAGAGGCAAAGACCCATCAGATCCAGTCGACTATCCAGACAAGCAGATCACTTGGAATGGTAACTATGGATGAT
TCACTCTATGAGCTATATGCGTCAGGCACAGTAACAAGAGAGACGGCCATGACATATGCTCAGGATCAGCAGTATCTGAG
AAAGAAGATCAATGGACAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.424

93.484

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.424

93.484

0.49
  pilT Acinetobacter baylyi ADP1

50.882

96.317

0.49
  pilT Pseudomonas aeruginosa PAK

50

97.45

0.487
  pilT Legionella pneumophila strain Lp02

51.045

94.901

0.484
  pilT Legionella pneumophila strain ERS1305867

51.045

94.901

0.484
  pilT Acinetobacter nosocomialis M2

49.422

98.017

0.484
  pilT Neisseria meningitidis 8013

49.133

98.017

0.482
  pilT Acinetobacter baumannii strain A118

50

96.317

0.482
  pilT Acinetobacter baumannii D1279779

50

96.317

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.571

99.15

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.118

96.317

0.473
  pilT Neisseria gonorrhoeae MS11

48.406

97.734

0.473
  pilU Pseudomonas stutzeri DSM 10701

40.688

98.867

0.402
  pilU Vibrio cholerae strain A1552

40.751

98.017

0.399
  pilU Acinetobacter baylyi ADP1

40

99.15

0.397