Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FH508_RS06490 Genome accession   NZ_CP085880
Coordinates   1388038..1389087 (+) Length   349 a.a.
NCBI ID   WP_173478565.1    Uniprot ID   -
Organism   Lysinibacillus sp. CD3-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1383038..1394087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH508_RS06470 (FH508_0006475) - 1383687..1384262 (-) 576 WP_173478211.1 type II secretion system protein J -
  FH508_RS06475 (FH508_0006480) - 1384249..1384701 (-) 453 WP_173478212.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FH508_RS06480 (FH508_0006485) - 1384993..1386333 (+) 1341 WP_173478213.1 VanW family protein -
  FH508_RS06485 (FH508_0006490) - 1386352..1388022 (+) 1671 WP_173478214.1 GspE/PulE family protein -
  FH508_RS06490 (FH508_0006495) pilT 1388038..1389087 (+) 1050 WP_173478565.1 type IV pilus twitching motility protein PilT Machinery gene
  FH508_RS06495 (FH508_0006500) - 1389084..1390289 (+) 1206 WP_173478215.1 type II secretion system F family protein -
  FH508_RS06500 (FH508_0006505) - 1390394..1390855 (+) 462 WP_173478216.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FH508_RS06505 (FH508_0006510) pilM 1390976..1391950 (+) 975 WP_173478217.1 type IV pilus biogenesis protein PilM -
  FH508_RS06510 (FH508_0006515) - 1391950..1392543 (+) 594 WP_173478218.1 PilN domain-containing protein -
  FH508_RS06515 (FH508_0006520) - 1392540..1393250 (+) 711 WP_173478219.1 potassium transporter -
  FH508_RS06520 (FH508_0006525) - 1393309..1394061 (+) 753 WP_173478220.1 A24 family peptidase -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 39068.77 Da        Isoelectric Point: 6.8736

>NTDB_id=621097 FH508_RS06490 WP_173478565.1 1388038..1389087(+) (pilT) [Lysinibacillus sp. CD3-6]
MTTSRNIEELLTRSFEEKASDLHLTKGIPPVYRIHGQLEHYGTEPLTSEELQTMVEAILPEHKRQEFHTKGETDFNYSLP
GKCRFRVNAYHQRNEMTIAARLIEAKIPSIESLNMPQVLYQLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETKSKHII
TLEDPIEYLHTHKKSVINQREVGVDTQSFANGLRAALRQDPDMILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTT
IDRIIDVFPPHQQGQIRVQLANVLVGIISQRLFIRKDAKGRIAATEILVQAPSIANLIRNEKVHQIPSVMQTSRALGMHT
LESSMQQLIAMGKISLEDARPYMNAGEYQ

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=621097 FH508_RS06490 WP_173478565.1 1388038..1389087(+) (pilT) [Lysinibacillus sp. CD3-6]
ATGACAACATCAAGAAATATAGAAGAGCTATTGACACGTTCATTTGAGGAAAAGGCGTCAGATTTACATTTAACAAAAGG
TATTCCTCCTGTATATCGCATCCATGGTCAGTTGGAGCATTACGGAACTGAACCGCTTACTTCAGAAGAATTACAAACGA
TGGTGGAAGCTATTTTACCTGAACATAAACGACAAGAATTTCATACTAAAGGTGAAACAGATTTTAACTATTCATTGCCC
GGGAAATGTCGTTTTCGTGTAAATGCCTATCATCAACGAAATGAAATGACAATCGCCGCACGTTTAATAGAAGCAAAAAT
TCCGTCCATTGAATCGTTAAATATGCCTCAAGTGTTGTATCAGCTTGCTGAAAAACCACAGGGACTTATTTTAGTAACAG
GTCCTACAGGTTCTGGGAAATCGACAACACTAGCGGCGATGATTGACTACATTAATGAAACAAAATCAAAGCATATTATT
ACACTTGAAGACCCAATTGAGTATTTACATACACATAAAAAATCGGTCATCAATCAACGTGAAGTAGGTGTCGATACACA
AAGCTTTGCCAATGGTTTGCGTGCTGCACTACGTCAGGACCCTGACATGATATTAGTCGGTGAGATGCGTGACTATGAAA
CAATCTCAACTGCTATTACAGCCGCTGAAACAGGTCACTTAGTGATGGCAACATTGCATACAAGTAGTGCGCCAACAACG
ATTGATCGTATTATTGATGTCTTTCCACCACATCAGCAAGGACAAATTCGTGTACAGTTAGCCAATGTGTTAGTAGGTAT
TATTTCGCAGCGTTTATTTATTCGAAAAGATGCAAAGGGGCGAATTGCAGCAACTGAAATATTAGTACAGGCACCGTCTA
TTGCGAACTTAATCCGCAATGAAAAGGTCCACCAAATTCCAAGTGTCATGCAGACAAGCCGTGCGCTCGGCATGCATACA
TTGGAGTCCTCGATGCAGCAGTTAATTGCCATGGGGAAAATTTCACTGGAGGATGCAAGACCATATATGAATGCGGGTGA
GTATCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(84-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.976

94.269

0.49
  pilT Pseudomonas aeruginosa PAK

50.149

95.989

0.481
  pilT Neisseria meningitidis 8013

50.299

95.702

0.481
  pilT Neisseria gonorrhoeae MS11

50

95.702

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.552

95.989

0.476
  pilT Vibrio cholerae strain A1552

49.552

95.989

0.476
  pilT Acinetobacter baylyi ADP1

48.246

97.994

0.473
  pilT Acinetobacter baumannii strain A118

49.254

95.989

0.473
  pilT Acinetobacter baumannii D1279779

49.254

95.989

0.473
  pilT Acinetobacter nosocomialis M2

48.955

95.989

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.955

95.989

0.47
  pilT Legionella pneumophila strain Lp02

47.273

94.556

0.447
  pilT Legionella pneumophila strain ERS1305867

47.273

94.556

0.447
  pilU Pseudomonas stutzeri DSM 10701

43.544

95.415

0.415
  pilU Vibrio cholerae strain A1552

43.478

92.264

0.401
  pilU Acinetobacter baylyi ADP1

39.003

97.708

0.381