Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LJQ72_RS17110 Genome accession   NZ_CP085633
Coordinates   3799334..3800350 (-) Length   338 a.a.
NCBI ID   WP_227527237.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain TS20HJ1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3794334..3805350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJQ72_RS17095 (LJQ72_17095) nsrR 3794666..3795091 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  LJQ72_RS17100 (LJQ72_17100) - 3795435..3797006 (+) 1572 WP_040044022.1 methyl-accepting chemotaxis protein -
  LJQ72_RS17105 (LJQ72_17105) - 3797533..3799365 (+) 1833 WP_104212826.1 diguanylate cyclase -
  LJQ72_RS17110 (LJQ72_17110) pilT 3799334..3800350 (-) 1017 WP_227527237.1 type IV pilus twitching motility protein PilT Machinery gene
  LJQ72_RS17115 (LJQ72_17115) - 3800371..3801084 (+) 714 WP_227527238.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJQ72_RS17120 (LJQ72_17120) proC 3801179..3802000 (+) 822 WP_227528319.1 pyrroline-5-carboxylate reductase -
  LJQ72_RS17125 (LJQ72_17125) - 3802049..3802603 (+) 555 WP_211024281.1 YggT family protein -
  LJQ72_RS17130 (LJQ72_17130) yggU 3802600..3802890 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  LJQ72_RS17135 (LJQ72_17135) - 3802908..3803501 (+) 594 WP_227527239.1 XTP/dITP diphosphatase -
  LJQ72_RS17140 (LJQ72_17140) hemW 3803494..3804636 (+) 1143 WP_227527240.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36639.85 Da        Isoelectric Point: 6.8190

>NTDB_id=618986 LJQ72_RS17110 WP_227527237.1 3799334..3800350(-) (pilT) [Pectobacterium brasiliense strain TS20HJ1]
MGLDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLAPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLAELQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLFPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=618986 LJQ72_RS17110 WP_227527237.1 3799334..3800350(-) (pilT) [Pectobacterium brasiliense strain TS20HJ1]
ATGGGGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGCACCCGCTCAGCGCGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTATTTCGGCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGTTCAACCTTCATT
GGCGGAGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGTCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGTTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATTGGTGCGCACAGTGCGTCCTTTGC
GCAGGCGCTACGTGCTGCATTGCGGGAGGATCCCGATGTTATTCTGCTGGGCGAGTTGCGCGATACGGAGACGATTCGAC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGCTGTTTCCCGCTGCGCAAGGTGGTCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.64

97.929

0.476
  pilT Legionella pneumophila strain ERS1305867

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.338

97.929

0.473
  pilT Acinetobacter baumannii strain A118

48.338

97.929

0.473
  pilT Acinetobacter nosocomialis M2

48.338

97.929

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.272

95.562

0.423
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367