Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LJF99_RS10575 Genome accession   NZ_CP085533
Coordinates   2297489..2298523 (+) Length   344 a.a.
NCBI ID   WP_005320466.1    Uniprot ID   A0AAP4JDF8
Organism   Aeromonas salmonicida subsp. salmonicida strain 890054     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2292489..2303523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJF99_RS10545 (LJF99_10550) - 2294148..2294447 (+) 300 WP_011899099.1 hypothetical protein -
  LJF99_RS10550 (LJF99_10555) - 2294516..2294935 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  LJF99_RS10555 (LJF99_10560) yggU 2294954..2295253 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  LJF99_RS10560 (LJF99_10565) - 2295253..2295804 (-) 552 WP_005320477.1 YggT family protein -
  LJF99_RS10565 (LJF99_10570) proC 2295833..2296657 (-) 825 WP_005320475.1 pyrroline-5-carboxylate reductase -
  LJF99_RS10570 (LJF99_10575) - 2296748..2297449 (-) 702 WP_005320467.1 YggS family pyridoxal phosphate-dependent enzyme -
  LJF99_RS10575 (LJF99_10580) pilT 2297489..2298523 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  LJF99_RS10580 (LJF99_10585) pilU 2298559..2299668 (+) 1110 WP_005320465.1 type IVa pilus ATPase TapU Machinery gene
  LJF99_RS10585 (LJF99_10590) yaaA 2299700..2300473 (+) 774 WP_005320464.1 peroxide stress protein YaaA -
  LJF99_RS10590 (LJF99_10595) srmB 2300662..2301885 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  LJF99_RS10595 (LJF99_10600) - 2302014..2302724 (+) 711 WP_005320460.1 methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38307.00 Da        Isoelectric Point: 6.7439

>NTDB_id=618890 LJF99_RS10575 WP_005320466.1 2297489..2298523(+) (pilT) [Aeromonas salmonicida subsp. salmonicida strain 890054]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQSRGSGAVFRTIPSTVLSLEDLDAPEIFRKISEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=618890 LJF99_RS10575 WP_005320466.1 2297489..2298523(+) (pilT) [Aeromonas salmonicida subsp. salmonicida strain 890054]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTACCCGCCCTGGAACACAGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTCGACTTCTCGTTCGAAGTCCCCAATCTGGCCCGC
TTCCGGGTCAATGCCTTCCAGCAATCCCGCGGTTCGGGGGCCGTATTCCGTACCATCCCCAGCACGGTGCTGAGCCTGGA
GGATCTCGATGCCCCCGAAATCTTCCGCAAGATCTCCGAATACCCGCGCGGCCTGGTACTGGTGACAGGACCGACCGGCT
CGGGCAAGTCCACCACACTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATACTCACCATCGAAGAC
CCCATCGAATTCGTGCACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTTCACAGGGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATACTGGTGGGCGAGATGCGTGACCTCGAGACCATTCGTCTGG
CAATGACGGCGGCCGAGACCGGCCATCTGGTATTCGGCACCCTGCACACCTCGTCGGCAGCCAAGACCATAGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAACGGATAGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATACCCGCTGTGCGCAACCTCATAC
GGGAAGACAAGATCGCCCAGCTCTACTCCGTGATCCAGACCGGGATGACCCATGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTCAGCCGTGGTGTGGTCGCCTCACTCGATGCCAAGGCCAAGGCGGTCGACCCCAATAGCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.843

99.709

0.776
  pilT Acinetobacter baylyi ADP1

77.843

99.709

0.776
  pilT Acinetobacter baumannii D1279779

77.551

99.709

0.773
  pilT Acinetobacter baumannii strain A118

77.551

99.709

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.385

99.709

0.762
  pilT Pseudomonas aeruginosa PAK

75.802

99.709

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.668

97.965

0.741
  pilT Vibrio cholerae strain A1552

75.668

97.965

0.741
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

42.609

100

0.427
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404