Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG308_RS18965 Genome accession   NZ_CP085257
Coordinates   4113190..4114224 (-) Length   344 a.a.
NCBI ID   WP_222909779.1    Uniprot ID   -
Organism   Stutzerimonas chloritidismutans strain ABH-495     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4108190..4119224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG308_RS18945 (LG308_18910) - 4109372..4109770 (-) 399 WP_404438631.1 NINE protein -
  LG308_RS18950 (LG308_18915) - 4109909..4111126 (+) 1218 WP_248494037.1 putative DNA modification/repair radical SAM protein -
  LG308_RS18955 (LG308_18920) - 4111126..4111965 (+) 840 WP_404438632.1 TIGR03915 family putative DNA repair protein -
  LG308_RS18960 (LG308_18925) pilU 4111966..4113111 (-) 1146 WP_045164002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LG308_RS18965 (LG308_18930) pilT 4113190..4114224 (-) 1035 WP_222909779.1 type IV pilus twitching motility protein PilT Machinery gene
  LG308_RS18970 (LG308_18935) - 4114291..4114965 (+) 675 Protein_3721 YggS family pyridoxal phosphate-dependent enzyme -
  LG308_RS18975 (LG308_18940) proC 4115017..4115844 (+) 828 WP_404441691.1 pyrroline-5-carboxylate reductase -
  LG308_RS18980 (LG308_18945) - 4116121..4116711 (+) 591 WP_222909782.1 YggT family protein -
  LG308_RS18985 (LG308_18950) - 4116833..4118794 (+) 1962 WP_404438634.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37919.65 Da        Isoelectric Point: 6.8360

>NTDB_id=616671 LG308_RS18965 WP_222909779.1 4113190..4114224(-) (pilT) [Stutzerimonas chloritidismutans strain ABH-495]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=616671 LG308_RS18965 WP_222909779.1 4113190..4114224(-) (pilT) [Stutzerimonas chloritidismutans strain ABH-495]
ATGGATATCACAGAGCTGCTGGCCTTCAGTGCCAAGCAGGGTGCATCGGATTTGCACCTGTCTTCCGGCTTGCCGCCGAT
GATCCGCGTCGATGGCGATGTACGTCGGATCAACCTCCCCGCGATGGACCATAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGTAAGGATTTCGAGGAATTTCTCGAGACCGACTTCTCGTTCGAAGTTCCGGGTGTCGCGCGT
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCTGGTGCCGTGTTCCGGACCATTCCGTCGAAGGTCCTGACCATGGA
AGACCTCGGGATGGGGGAGGTCTTCCGCAAGATCACCGATGTGCCGCGCGGACTGGTGCTGGTGACCGGTCCAACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTCAACAGCACCAAGTATCACCACATTCTCACCATCGAAGAC
CCAATCGAATTCGTCCATGAATCGAAAAAGTGTCTGGTCAACCAACGCGAAGTTCACCGTGACACGCTCGGCTTCTCTGA
GGCCTTGCGCTCGGCTCTGCGGGAAGACCCGGACATCATCCTCGTCGGTGAAATGCGTGACCTGGAGACCATCCGTTTGG
CGCTGACTGCCGCAGAAACAGGCCACCTGGTGTTTGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTG
GTTGACGTATTCCCGGCGGAAGAAAAATCCATGGTCCGCTCGATGCTCTCCGAGTCGCTGCAGGCGGTGATTTCCCAGAC
GTTGCTCAAGAAGGTCGGCGGTGGCCGCGTTGCGGCACACGAGATCATGATTGGCACGCCGGCGATCCGGAACCTGATTC
GTGAAGACAAAGTCGCGCAGATGTACTCGTCGATCCAGACCGGTGGTTCATTGGGCATGCAAACGCTCGATTCCTGCCTG
AAAGGCCTGCTTGCCAAGGGCCTGATTTCACGCGATAGCGCAAAAGAAAAAGCCAAACAGCCAGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.709

100

0.997
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392