Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LG289_RS07700 Genome accession   NZ_CP085014
Coordinates   1476970..1478010 (+) Length   346 a.a.
NCBI ID   WP_404337435.1    Uniprot ID   -
Organism   Planococcus rifietoensis strain SLM-623     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1471970..1483010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG289_RS07675 (LG289_07670) - 1472090..1472626 (-) 537 WP_404337425.1 type II secretion system protein J -
  LG289_RS07680 (LG289_07675) - 1472607..1473110 (-) 504 WP_404337427.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LG289_RS07685 (LG289_07680) - 1473495..1473854 (+) 360 WP_404337429.1 hypothetical protein -
  LG289_RS07690 (LG289_07685) - 1473856..1475283 (+) 1428 WP_404337431.1 hypothetical protein -
  LG289_RS07695 (LG289_07690) - 1475304..1476965 (+) 1662 WP_404337433.1 GspE/PulE family protein -
  LG289_RS07700 (LG289_07695) pilT 1476970..1478010 (+) 1041 WP_404337435.1 type IV pilus twitching motility protein PilT Machinery gene
  LG289_RS07705 (LG289_07700) - 1478011..1479222 (+) 1212 WP_404337437.1 type II secretion system F family protein -
  LG289_RS07710 (LG289_07705) - 1479351..1479821 (+) 471 WP_404337439.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LG289_RS07715 (LG289_07710) pilM 1479854..1480792 (+) 939 WP_404337441.1 type IV pilus biogenesis protein PilM -
  LG289_RS07720 (LG289_07715) - 1480796..1481533 (+) 738 WP_404337443.1 fimbrial assembly protein -
  LG289_RS07725 (LG289_07720) - 1481530..1482285 (+) 756 WP_404337445.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37718.38 Da        Isoelectric Point: 7.3356

>NTDB_id=614529 LG289_RS07700 WP_404337435.1 1476970..1478010(+) (pilT) [Planococcus rifietoensis strain SLM-623]
MPTTYIDKVLTQARNLNVSDIHLTTGIPPVFRMNGTLKRYGDENLTPDMTKEIAKALMPEILWQQFLDKGEMDFSYSLSG
VARFRVNSFHQRGSISHAFRTIPTEIPTIADLQMPKTLANLAETQQGLILVTGPTGSGKSTTLAAMIRHINENLTKHIIT
LEDPIEYLHRHGSSIIDQREVGFDTGSFAAGLRAALRQDPDVVLVGEMRDLETISTAITAAETGHLVMATLHTSSAASTI
ERIIDVFPAGQQAQVRTQLAGILKAVVSQRLLPTADGTGRIAATEILINNPAVANLIRTEKVHQIPNVILTNRSFGMHMM
VSSVQELLAAGKISRPTAQPFLEGGK

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=614529 LG289_RS07700 WP_404337435.1 1476970..1478010(+) (pilT) [Planococcus rifietoensis strain SLM-623]
ATGCCAACCACTTATATAGACAAAGTCTTGACGCAAGCACGCAACTTAAATGTCTCAGATATTCATTTGACGACTGGCAT
TCCGCCCGTTTTCCGTATGAACGGCACGTTGAAACGCTATGGCGATGAGAACTTGACACCTGACATGACCAAGGAAATCG
CTAAAGCATTGATGCCGGAAATTCTATGGCAGCAATTTCTCGATAAAGGCGAAATGGACTTTTCCTACTCGCTTTCCGGG
GTTGCCCGCTTCCGCGTCAATTCTTTCCACCAGCGCGGTTCGATTTCGCATGCCTTCCGGACGATCCCAACAGAAATCCC
GACAATCGCTGATTTGCAGATGCCGAAAACCTTGGCGAATTTGGCAGAAACACAGCAAGGCCTCATTTTGGTGACAGGGC
CGACTGGCTCAGGGAAGTCGACTACCTTGGCGGCAATGATTCGCCACATCAACGAAAATTTGACCAAACACATCATCACT
TTAGAAGACCCGATTGAGTATTTGCATCGCCACGGCTCGTCAATCATCGACCAGCGTGAAGTCGGTTTTGATACAGGTTC
TTTCGCCGCCGGTTTACGCGCAGCTTTGCGCCAAGACCCGGATGTTGTTTTGGTCGGGGAAATGCGCGATTTGGAAACGA
TTTCGACCGCCATCACCGCCGCTGAAACCGGTCATTTGGTGATGGCAACATTGCATACGTCGAGTGCAGCTTCGACGATT
GAACGAATCATTGACGTCTTTCCCGCAGGACAGCAGGCGCAAGTACGCACACAACTTGCCGGTATTCTAAAAGCGGTGGT
GTCCCAGCGCTTGCTGCCTACTGCCGACGGCACAGGACGAATCGCGGCTACCGAGATCCTCATCAACAACCCAGCCGTTG
CGAACCTGATTCGAACTGAAAAAGTTCATCAAATTCCGAATGTCATTTTGACAAACCGCTCATTCGGCATGCATATGATG
GTGTCGTCCGTCCAGGAATTATTGGCAGCTGGCAAAATCAGCCGTCCGACCGCCCAACCATTCCTTGAGGGGGGCAAGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.968

91.04

0.491
  pilT Neisseria meningitidis 8013

50.61

94.798

0.48
  pilT Neisseria gonorrhoeae MS11

50.305

94.798

0.477
  pilT Pseudomonas stutzeri DSM 10701

48.387

98.555

0.477
  pilT Pseudomonas aeruginosa PAK

47.214

98.555

0.465
  pilT Vibrio cholerae strain A1552

48.93

94.509

0.462
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

94.509

0.462
  pilT Acinetobacter baylyi ADP1

49.221

92.775

0.457
  pilT Acinetobacter baumannii strain A118

48.91

92.775

0.454
  pilT Acinetobacter baumannii D1279779

48.91

92.775

0.454
  pilT Acinetobacter nosocomialis M2

48.598

92.775

0.451
  pilT Legionella pneumophila strain Lp02

47.84

93.642

0.448
  pilT Legionella pneumophila strain ERS1305867

47.84

93.642

0.448
  pilU Pseudomonas stutzeri DSM 10701

44.375

92.486

0.41
  pilU Vibrio cholerae strain A1552

40.12

96.532

0.387
  pilU Acinetobacter baylyi ADP1

40.81

92.775

0.379