Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6742_RS10080 Genome accession   NZ_CP084692
Coordinates   2150800..2151780 (+) Length   326 a.a.
NCBI ID   WP_214594614.1    Uniprot ID   -
Organism   Enterobacter mori strain BC01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2145800..2156780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6742_RS10040 (K6742_10040) - 2145883..2146209 (+) 327 WP_010435485.1 YggL family protein -
  K6742_RS10045 (K6742_10045) - 2146267..2146983 (+) 717 WP_126815894.1 DUF2884 domain-containing protein -
  K6742_RS10050 (K6742_10050) - 2147066..2147404 (-) 339 WP_108416266.1 endonuclease domain-containing protein -
  K6742_RS10055 (K6742_10055) hemW 2147468..2148610 (-) 1143 WP_212474514.1 radical SAM family heme chaperone HemW -
  K6742_RS10060 (K6742_10060) - 2148603..2149196 (-) 594 WP_010435473.1 XTP/dITP diphosphatase -
  K6742_RS10065 (K6742_10065) yggU 2149200..2149496 (-) 297 WP_010435470.1 DUF167 family protein YggU -
  K6742_RS10070 (K6742_10070) - 2149493..2150059 (-) 567 WP_010435467.1 YggT family protein -
  K6742_RS10075 (K6742_10075) - 2150081..2150782 (-) 702 WP_214594615.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6742_RS10080 (K6742_10080) pilT 2150800..2151780 (+) 981 WP_214594614.1 type IV pilus twitching motility protein PilT Machinery gene
  K6742_RS10085 (K6742_10085) ruvX 2151791..2152207 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  K6742_RS10090 (K6742_10090) - 2152207..2152767 (-) 561 WP_126815898.1 YqgE/AlgH family protein -
  K6742_RS10095 (K6742_10095) gshB 2152842..2153789 (-) 948 WP_010435454.1 glutathione synthase -
  K6742_RS10100 (K6742_10100) rsmE 2153809..2154540 (-) 732 WP_148400660.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6742_RS10105 (K6742_10105) endA 2154592..2155299 (-) 708 WP_214594613.1 deoxyribonuclease I -
  K6742_RS10110 (K6742_10110) - 2155394..2155891 (-) 498 WP_010435446.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35639.67 Da        Isoelectric Point: 6.1733

>NTDB_id=612409 K6742_RS10080 WP_214594614.1 2150800..2151780(+) (pilT) [Enterobacter mori strain BC01]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRG
SAFTHMKGVSIALRLLPLACPQLSALGVPRAIPELLSSDSGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSLSFAEALRSALREDPDIILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRHQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=612409 K6742_RS10080 WP_214594614.1 2150800..2151780(+) (pilT) [Enterobacter mori strain BC01]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCGGCCCCGTTTCCAGCCCCCGACGTTGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGACTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCATATGAAGGGAGTGTCGATCGCCTTGCGCCTGTTACCGCTCGCGTGTCCGCAGCTTTCTGCTCTGGG
TGTGCCGAGGGCTATCCCGGAACTGCTCTCCAGTGACAGCGGGTTGATTCTGGTGACGGGTGCGACCGGTAGCGGAAAAT
CCACGACGCTCGCGGCGATGGTGGATTTTCTCAACCATCACACCGACGGCCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGCTGCCTGATACAGCAGCGGGAGATTGGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
CAGTGCGCTGCGCGAAGATCCGGACATTATTCTGTTGGGAGAGCTGCGTGACAGCGAAACCATCCGCCTTGCGTTGACGG
CGGCCGAAACCGGGCACCTGGTGCTGGCGACGCTGCATACACGCGGCGCTTCGCAGGCGATTGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTCACCAGCTGGCAGGCAGCCTGCGCGCTGTACTGGCGCAAAAACTGCTTCA
GGATGTGCAGGGCGGGCGCGTGGCGCTTTATGAACTGCTGGTGAACACCTCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AGACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAAAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilB Haemophilus influenzae 86-028NP

33.428

100

0.362