Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LGL95_RS04005 Genome accession   NZ_CP084654
Coordinates   898354..899370 (+) Length   338 a.a.
NCBI ID   WP_103861772.1    Uniprot ID   -
Organism   Pectobacterium versatile strain SR12     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 893354..904370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGL95_RS03975 (LGL95_03975) hemW 894062..895201 (-) 1140 WP_211006917.1 radical SAM family heme chaperone HemW -
  LGL95_RS03980 (LGL95_03980) - 895194..895787 (-) 594 WP_226306170.1 XTP/dITP diphosphatase -
  LGL95_RS03985 (LGL95_03985) yggU 895819..896109 (-) 291 WP_010295005.1 DUF167 family protein YggU -
  LGL95_RS03990 (LGL95_03990) - 896106..896660 (-) 555 WP_010295003.1 YggT family protein -
  LGL95_RS03995 (LGL95_03995) proC 896710..897531 (-) 822 WP_180744160.1 pyrroline-5-carboxylate reductase -
  LGL95_RS04000 (LGL95_04000) - 897619..898332 (-) 714 WP_010294998.1 YggS family pyridoxal phosphate-dependent enzyme -
  LGL95_RS04005 (LGL95_04005) pilT 898354..899370 (+) 1017 WP_103861772.1 type IV pilus twitching motility protein PilT Machinery gene
  LGL95_RS04010 (LGL95_04010) - 899339..901171 (-) 1833 WP_180783487.1 diguanylate cyclase -
  LGL95_RS04015 (LGL95_04015) - 901824..903395 (-) 1572 WP_103861769.1 methyl-accepting chemotaxis protein -
  LGL95_RS04020 (LGL95_04020) nsrR 903739..904164 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36818.10 Da        Isoelectric Point: 6.7126

>NTDB_id=612298 LGL95_RS04005 WP_103861772.1 898354..899370(+) (pilT) [Pectobacterium versatile strain SR12]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPPVFSTLLEKPNGLLLITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=612298 LGL95_RS04005 WP_103861772.1 898354..899370(+) (pilT) [Pectobacterium versatile strain SR12]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCAGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGATGGAGCATTAATGCTGCCTGATGGGCAA
CGCCTGCGGGTGAACGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGCGTTATTCCATCCGTTCAACCTTCATT
GGACGCGCTACACGCGCCACCTGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGCTCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACAACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATGCTAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTGC
GCAGGCATTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AAAGCTGTTGCCTGCTGCGCAAGGGGGGCGAATCGCGCTTTATGAAGTACTGACCGCCACCGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.121

97.633

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

97.633

0.509
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.136

99.704

0.42
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364