Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LDO52_RS10985 Genome accession   NZ_CP084300
Coordinates   2269805..2270926 (+) Length   373 a.a.
NCBI ID   WP_225553022.1    Uniprot ID   -
Organism   Acinetobacter pseudolwoffii strain XMC5X702     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2264805..2275926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO52_RS10970 (LDO52_10970) - 2266790..2267893 (+) 1104 WP_225554245.1 acyltransferase family protein -
  LDO52_RS10975 (LDO52_10975) - 2267927..2268613 (-) 687 WP_225554247.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDO52_RS10980 (LDO52_10980) pilT 2268737..2269774 (+) 1038 WP_005104787.1 type IV pilus twitching motility protein PilT Machinery gene
  LDO52_RS10985 (LDO52_10985) pilU 2269805..2270926 (+) 1122 WP_225553022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDO52_RS10990 (LDO52_10990) fur 2270993..2271430 (-) 438 WP_005096051.1 ferric iron uptake transcriptional regulator -
  LDO52_RS10995 (LDO52_10995) - 2271543..2271941 (+) 399 WP_004279275.1 outer membrane protein assembly factor BamE -
  LDO52_RS11000 (LDO52_11000) - 2272042..2272374 (-) 333 WP_225553024.1 RnfH family protein -
  LDO52_RS11005 (LDO52_11005) - 2272374..2273453 (-) 1080 WP_225553026.1 hypothetical protein -
  LDO52_RS11010 (LDO52_11010) - 2273597..2274052 (-) 456 WP_005096048.1 bacteriohemerythrin -
  LDO52_RS11015 (LDO52_11015) - 2274334..2275182 (-) 849 WP_225553028.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41660.92 Da        Isoelectric Point: 6.7815

>NTDB_id=610523 LDO52_RS10985 WP_225553022.1 2269805..2270926(+) (pilU) [Acinetobacter pseudolwoffii strain XMC5X702]
MDFNGLLEYMVEKKASDLFITADVEPSIKVNGQILPIGSVKLPGAAVGQLLHSIMTEKQRKEFADSRECNFAISTLDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIILFVGGTGTGKSTSLASIIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFETERHNQLFMDLSLNMKAIVAQQLIPTVDGTGRRAAIEILINSPLVADLIRKGHVDEIKELMTRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLAEEGGSHLLRAHSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=610523 LDO52_RS10985 WP_225553022.1 2269805..2270926(+) (pilU) [Acinetobacter pseudolwoffii strain XMC5X702]
ATGGATTTTAACGGCTTACTCGAATATATGGTGGAGAAAAAAGCATCGGATCTGTTCATTACAGCCGATGTCGAACCCTC
GATCAAAGTCAATGGGCAGATTTTACCGATTGGTTCAGTAAAATTACCAGGTGCGGCAGTAGGACAACTACTCCATTCCA
TCATGACCGAAAAGCAGCGTAAAGAATTTGCTGACAGTCGTGAATGTAACTTTGCCATAAGCACGCTGGATAAAAGTGCC
CGCTTCCGTGTCAGTGCCTTTCAGCAACGCGATCAACCGGGTATGGTGTTGCGTCGGATTGAAACCGTCATTCCAACCAT
GGATGAGTTGAAATTACCGCCAATTTTAAAAGAACTGGCGATGACCAAACGGGGCATTATTCTGTTTGTAGGGGGAACGG
GTACCGGTAAATCTACCTCTTTGGCCTCGATTATTGGCTATCGTAACGAAAACTCCAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTGCATCAGCATAAGGGCTGTATCATTACCCAGCGTGAAGTCGGCATTGATACGGATTCATTTGA
AATTGCCCTGAAAAACACCTTGCGTCAGGCACCGGATGTGATTCTGATTGGTGAGATCCGTTCGCGTGAAACCATGGATT
ATGCGATTGCCTTTGCCGAAACCGGGCATCTGGTCTTTGCTACCCTGCACGCCAACAACGCCAACCAGGCCATTGACCGG
ATCATCCATTTCTTTGAAACTGAACGGCACAACCAGTTGTTTATGGACCTGTCGTTAAACATGAAAGCGATTGTGGCGCA
GCAACTGATTCCAACCGTCGATGGCACTGGCCGTCGTGCAGCCATTGAGATTCTGATTAACTCACCACTGGTGGCCGACT
TGATCCGTAAAGGTCATGTGGATGAGATCAAGGAACTGATGACGCGTTCCCGCGAACTGGGGATGCAGACCTTCGATCAG
GCGCTGTATGATTTGTATAAAGCCAAACAGATCAGCTATAAAGATGCGCTGAAACATGCGGATTCACCCAATGACTTACG
TCTGCAAATCAAACTGGCAGAAGAAGGCGGCAGTCATTTATTACGTGCACATTCCAATATCACCTTTGATGGCCAGTCCT
GA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

71.164

100

0.721
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

57.55

94.102

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.642

92.761

0.405
  pilT Legionella pneumophila strain Lp02

43.155

90.08

0.389
  pilT Legionella pneumophila strain ERS1305867

43.155

90.08

0.389
  pilT Acinetobacter nosocomialis M2

41.983

91.957

0.386
  pilT Acinetobacter baumannii D1279779

41.983

91.957

0.386
  pilT Acinetobacter baumannii strain A118

41.983

91.957

0.386
  pilT Neisseria gonorrhoeae MS11

40.936

91.689

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.886

94.102

0.375
  pilT Vibrio cholerae strain A1552

39.886

94.102

0.375
  pilT Pseudomonas aeruginosa PAK

40.462

92.761

0.375
  pilT Neisseria meningitidis 8013

40.462

92.761

0.375
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.761

0.367