Detailed information NTG0609552

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: NML21_RS03985
• Genome accession: NZ_CP101251
• Coordinates: 819070..820050 (+)
• Length: 326 a.a.
• NCBI ID: WP_001324408.1
• Uniprot ID: -
• Organism: Escherichia coli strain EC21Z-083
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 814070..825050

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NML21_RS03955 (NML21_03965)	yggM	814585..815592 (+)	1008	WP_000745217.1	DUF1202 family protein	-
NML21_RS03960 (NML21_03970)	hemW	815747..816883 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
NML21_RS03965 (NML21_03975)	rdgB	816876..817469 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
NML21_RS03970 (NML21_03980)	yggU	817477..817767 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
NML21_RS03975 (NML21_03985)	yggT	817764..818330 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
NML21_RS03980 (NML21_03990)	yggS	818348..819052 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
NML21_RS03985 (NML21_03995)	pilT	819070..820050 (+)	981	WP_001324408.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NML21_RS03990 (NML21_04000)	ruvX	820213..820629 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
NML21_RS03995 (NML21_04005)	yqgE	820629..821192 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
NML21_RS04000 (NML21_04010)	gshB	821301..822251 (-)	951	WP_000593273.1	glutathione synthase	-
NML21_RS04005 (NML21_04015)	rsmE	822264..822995 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
NML21_RS04010 (NML21_04020)	endA	823075..823782 (-)	708	WP_000286500.1	deoxyribonuclease I	-
NML21_RS04015 (NML21_04025)	yggI	823877..824374 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35873.07 Da
• Isoelectric Point: 5.5857

>NTDB_id=609552 NML21_RS03985 WP_001324408.1 819070..820050(+) (pilT) [Escherichia coli strain EC21Z-083]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=609552 NML21_RS03985 WP_001324408.1 819070..820050(+) (pilT) [Escherichia coli strain EC21Z-083]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.177	100	0.463
pilT	Acinetobacter nosocomialis M2	46.177	100	0.463
pilT	Acinetobacter baumannii strain A118	46.177	100	0.463
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.21	100	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362