Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LCH15_RS23010 Genome accession   NZ_CP083938
Coordinates   4995669..4996712 (+) Length   347 a.a.
NCBI ID   WP_003068464.1    Uniprot ID   A0A5S4T2J0
Organism   Comamonas thiooxydans strain CSUSB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4990669..5001712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH15_RS22990 (LCH15_22990) - 4990940..4992538 (-) 1599 WP_063663604.1 AMP-binding protein -
  LCH15_RS22995 (LCH15_22995) - 4992703..4993473 (+) 771 WP_052057603.1 TetR/AcrR family transcriptional regulator -
  LCH15_RS23000 (LCH15_23000) - 4993520..4994767 (+) 1248 WP_224649101.1 branched-chain amino acid ABC transporter substrate-binding protein -
  LCH15_RS23005 (LCH15_23005) - 4994900..4995631 (-) 732 WP_224649103.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCH15_RS23010 (LCH15_23010) pilT 4995669..4996712 (+) 1044 WP_003068464.1 type IV pilus twitching motility protein PilT Machinery gene
  LCH15_RS23015 (LCH15_23015) pilU 4996837..4997973 (+) 1137 WP_003068462.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCH15_RS23020 (LCH15_23020) - 4998118..4999029 (+) 912 WP_253232624.1 NAD(P)-dependent oxidoreductase -
  LCH15_RS23025 (LCH15_23025) - 4999091..4999744 (-) 654 WP_003068458.1 BON domain-containing protein -
  LCH15_RS23030 (LCH15_23030) - 4999741..5000349 (-) 609 WP_003068456.1 SIS domain-containing protein -
  LCH15_RS23035 (LCH15_23035) - 5000439..5000840 (-) 402 WP_034354994.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38354.25 Da        Isoelectric Point: 6.7743

>NTDB_id=608631 LCH15_RS23010 WP_003068464.1 4995669..4996712(+) (pilT) [Comamonas thiooxydans strain CSUSB4]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHAMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNMGMQTLDQN
LTDLVRRNLISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=608631 LCH15_RS23010 WP_003068464.1 4995669..4996712(+) (pilT) [Comamonas thiooxydans strain CSUSB4]
GTGGATATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCGGCAGGCCTGCCCCCCAT
GATTCGCGTCCACGGCGATGTGCGCCGCATCAACATCGATCCGCTGGACCACAAGACCGTGCACGCCATGGTCTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTACGAAGAGTTTCTGGAAGTGGACTTTTCGTTCGAGATTGAAGGCCTGGCCCGG
TTTCGTGTCAATGCCTTCAACCAGAACCGCGGCGCGGCGGCCGTGTTCCGTACCATTCCCAGCAAGATCCTGACACTGGA
GCAACTCAACGCCCCCAAGATCTTCGCCGACCTGGCCCTGAAGCCACGTGGTCTGGTGCTGGTGACCGGTCCCACGGGTT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTATCTCAACGAGACCGAGTACGGCCACATCCTGACGGTGGAAGAC
CCCATCGAGTTCGTGCACGAGTCCAAGAAATGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTTGCGGC
AGCGCTGAAGTCCGCGCTGCGTGAAGACCCGGACGCGATCCTGGTCGGCGAAATGCGGGACCTGGAAACCATCCGTCTGG
CCATGACGGCGGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCGGCCGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAGTCGTTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGTGTGGCCGCACACGAGATCATGCTGGGCACACCCGCCATCCGAAACCTGA
TCCGAGAAGCCAAGGTGGCACAGATGTACTCCACCATTCAGACCAGCCAGAACATGGGCATGCAGACTCTGGACCAGAAC
CTGACCGATCTGGTGCGCCGCAACCTCATCAGTCCTGCCGAAGCGCGCAGCAAGGCGAAGATTCCCGAGAATTTTCCGGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4T2J0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409