Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAI70_RS24695 Genome accession   NZ_CP083719
Coordinates   2869593..2870636 (-) Length   347 a.a.
NCBI ID   WP_224445302.1    Uniprot ID   -
Organism   Cupriavidus metallidurans strain ZM02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2864593..2875636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAI70_RS24665 (LAI70_24665) - 2865573..2865959 (+) 387 WP_008641946.1 CidA/LrgA family protein -
  LAI70_RS24670 (LAI70_24670) - 2865956..2866681 (+) 726 WP_008641945.1 LrgB family protein -
  LAI70_RS24675 (LAI70_24675) - 2866767..2867147 (+) 381 WP_017514351.1 VOC family protein -
  LAI70_RS24680 (LAI70_24680) - 2867167..2867859 (+) 693 WP_008641943.1 helix-turn-helix transcriptional regulator -
  LAI70_RS24685 (LAI70_24685) - 2867886..2868380 (-) 495 WP_008641942.1 glutathione peroxidase -
  LAI70_RS24690 (LAI70_24690) pilU 2868436..2869575 (-) 1140 WP_224446158.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LAI70_RS24695 (LAI70_24695) pilT 2869593..2870636 (-) 1044 WP_224445302.1 type IV pilus twitching motility protein PilT Machinery gene
  LAI70_RS24700 (LAI70_24700) - 2870700..2871389 (+) 690 WP_224445303.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAI70_RS24705 (LAI70_24705) proC 2871471..2872292 (+) 822 WP_224446159.1 pyrroline-5-carboxylate reductase -
  LAI70_RS24710 (LAI70_24710) ubiA 2872344..2873228 (-) 885 WP_008641934.1 4-hydroxybenzoate octaprenyltransferase -
  LAI70_RS24715 (LAI70_24715) - 2873343..2873828 (-) 486 WP_008641932.1 Dps family protein -
  LAI70_RS24720 (LAI70_24720) - 2873914..2874105 (-) 192 WP_029307173.1 hypothetical protein -
  LAI70_RS24725 (LAI70_24725) - 2874273..2875229 (-) 957 WP_060784830.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38414.24 Da        Isoelectric Point: 6.9771

>NTDB_id=607451 LAI70_RS24695 WP_224445302.1 2869593..2870636(-) (pilT) [Cupriavidus metallidurans strain ZM02]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMSDVQRKSYEERLEVDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGMGMQTLDQC
LSDLIKRGMINYSDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=607451 LAI70_RS24695 WP_224445302.1 2869593..2870636(-) (pilT) [Cupriavidus metallidurans strain ZM02]
ATGGACATCGCGCAGCTATTGGCTTTTGCGGTCAAGAACAAGGCATCCGATCTCCACCTCTCGGCTGACATGCCGCCGAT
GGTGCGTATTCACGGGGATATGCGCCGCATCAACGTCGCGGCAATGACGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTTCAGCGCAAGTCTTACGAAGAGCGCCTTGAGGTCGATTTCTCCTTCGAGATCTCTGGTCTGTCCCGT
TTCCGGGTCAACGCCTATACCACCCAGCGCGGCGCGGCCGCGGTGTTCCGGACGATTCCGTCGAAGGTCCTGACGCTCGA
GGACCTGCGCGCGCCCGCCGTGTTCGCCGATCTGTGCATGAAGCCGCGTGGCCTGGTGCTGGTCACCGGGCCCACGGGCT
CGGGCAAGTCGACCACGCTCGCCGCGATGGTCGACCATCGCAACGACAACGACATGGGTCACATCCTCACGGTGGAGGAT
CCAATCGAATTCGTGCATCAGTCCAAAAAGAGTCTGATCAACCAGCGCGAGCTTGGCCCTCACACCCATTCGTTCGCCAA
CGCGCTGCGCTCGGCACTGCGCGAGGACCCGGACGTGGTTCTGGTGGGTGAATTGCGTGACCTGGAAACGATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTTTTCGCCACGCTGCACACGAGTTCGGCCGCCAAGACGATTGACCGTGTG
GTCGACGTGTTCCCGCCGGAAGAAAAGGACATGGTGCGAACCATGCTGTCGGAGTCGCTCGAGGCCGTGATCTCGCAGAC
GCTGCTCAAGACACGCGACGGCAACGGTCGGACGGCGGCACATGAAATCATGATCGCCACGCCTGCGATTCGCCACCTGA
TCCGCGAAAACAAGATCGCGCAGATGTACTCGATGATGCAGACGTCGAGCGGCATGGGCATGCAGACGCTGGACCAGTGC
CTGAGCGACCTGATCAAGCGGGGCATGATCAACTACAGCGACGCGCGGGCGATTGCGAAGAATCCCGACGCCTTCATGGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.387

99.712

0.712
  pilT Acinetobacter baumannii D1279779

71.387

99.712

0.712
  pilT Acinetobacter baumannii strain A118

71.387

99.712

0.712
  pilT Acinetobacter baylyi ADP1

69.275

99.424

0.689
  pilT Pseudomonas stutzeri DSM 10701

69.275

99.424

0.689
  pilT Pseudomonas aeruginosa PAK

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

66.86

99.135

0.663
  pilT Neisseria meningitidis 8013

66.86

99.135

0.663
  pilT Legionella pneumophila strain Lp02

66.087

99.424

0.657
  pilT Legionella pneumophila strain ERS1305867

66.087

99.424

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

99.424

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395
  pilU Vibrio cholerae strain A1552

40

96.542

0.386