Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GF111_RS16655 Genome accession   NZ_CP083690
Coordinates   3472611..3473633 (+) Length   340 a.a.
NCBI ID   WP_147190710.1    Uniprot ID   -
Organism   Serratia sp. HRI     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3467611..3478633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GF111_RS16620 (GF111_16620) - 3468302..3468385 (+) 84 Protein_3230 DUF2442 domain-containing protein -
  GF111_RS16625 (GF111_16625) hemW 3468423..3469565 (-) 1143 WP_060442859.1 radical SAM family heme chaperone HemW -
  GF111_RS16630 (GF111_16630) - 3469558..3470151 (-) 594 WP_047729848.1 XTP/dITP diphosphatase -
  GF111_RS16635 (GF111_16635) yggU 3470169..3470459 (-) 291 WP_047729849.1 DUF167 family protein YggU -
  GF111_RS16640 (GF111_16640) - 3470456..3471010 (-) 555 WP_047729850.1 YggT family protein -
  GF111_RS16645 (GF111_16645) proC 3471042..3471863 (-) 822 WP_048234669.1 pyrroline-5-carboxylate reductase -
  GF111_RS16650 (GF111_16650) - 3471883..3472590 (-) 708 WP_048234668.1 YggS family pyridoxal phosphate-dependent enzyme -
  GF111_RS16655 (GF111_16655) pilT 3472611..3473633 (+) 1023 WP_147190710.1 type IV pilus twitching motility protein PilT Machinery gene
  GF111_RS16660 (GF111_16660) ruvX 3473630..3474052 (-) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  GF111_RS16665 (GF111_16665) - 3474052..3474615 (-) 564 WP_033635940.1 YqgE/AlgH family protein -
  GF111_RS16670 (GF111_16670) gshB 3474761..3475711 (-) 951 WP_047729854.1 glutathione synthase -
  GF111_RS16675 (GF111_16675) rsmE 3475722..3476453 (-) 732 WP_033635938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GF111_RS16680 (GF111_16680) endA 3476523..3477218 (-) 696 WP_147190712.1 deoxyribonuclease I -
  GF111_RS16685 (GF111_16685) - 3477320..3477832 (-) 513 WP_016930082.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36393.69 Da        Isoelectric Point: 7.0670

>NTDB_id=607267 GF111_RS16655 WP_147190710.1 3472611..3473633(+) (pilT) [Serratia sp. HRI]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAASTLTAQGVQALCDGLLSAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAPPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=607267 GF111_RS16655 WP_147190710.1 3472611..3473633(+) (pilT) [Serratia sp. HRI]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGTCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGCCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTTCTGGTCACCGGCGCCACCG
GCAGCGGCAAATCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGTAGCCGACGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCTGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACTGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGATGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.636

97.059

0.521
  pilT Vibrio cholerae strain A1552

53.636

97.059

0.521
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.107

98.824

0.485
  pilT Neisseria gonorrhoeae MS11

49.096

97.647

0.479
  pilT Pseudomonas aeruginosa PAK

49.091

97.059

0.476
  pilT Legionella pneumophila strain ERS1305867

48.64

97.353

0.474
  pilT Legionella pneumophila strain Lp02

48.64

97.353

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.38

100

0.453
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.272

95.294

0.365