Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA332_RS23690 Genome accession   NZ_CP083630
Coordinates   4967671..4968648 (+) Length   325 a.a.
NCBI ID   WP_032613932.1    Uniprot ID   -
Organism   Leclercia adecarboxylata strain FDAARGOS_1505     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4962671..4973648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA332_RS23655 (LA332_23655) - 4962842..4963561 (+) 720 WP_032613965.1 DUF2884 domain-containing protein -
  LA332_RS23660 (LA332_23660) - 4963916..4964278 (-) 363 WP_032613945.1 endonuclease domain-containing protein -
  LA332_RS23665 (LA332_23665) hemW 4964339..4965481 (-) 1143 WP_032613943.1 radical SAM family heme chaperone HemW -
  LA332_RS23670 (LA332_23670) - 4965474..4966067 (-) 594 WP_032613941.1 XTP/dITP diphosphatase -
  LA332_RS23675 (LA332_23675) yggU 4966071..4966367 (-) 297 WP_032613939.1 DUF167 family protein YggU -
  LA332_RS23680 (LA332_23680) - 4966364..4966930 (-) 567 WP_032613937.1 YggT family protein -
  LA332_RS23685 (LA332_23685) - 4966952..4967653 (-) 702 WP_032613935.1 YggS family pyridoxal phosphate-dependent enzyme -
  LA332_RS23690 (LA332_23690) pilT 4967671..4968648 (+) 978 WP_032613932.1 type IV pilus twitching motility protein PilT Machinery gene
  LA332_RS23695 (LA332_23695) - 4968748..4969437 (+) 690 WP_032613957.1 hypothetical protein -
  LA332_RS23700 (LA332_23700) ruvX 4969476..4969892 (-) 417 WP_032613930.1 Holliday junction resolvase RuvX -
  LA332_RS23705 (LA332_23705) - 4969892..4970452 (-) 561 WP_032613928.1 YqgE/AlgH family protein -
  LA332_RS23710 (LA332_23710) gshB 4970544..4971491 (-) 948 WP_032613926.1 glutathione synthase -
  LA332_RS23715 (LA332_23715) rsmE 4971511..4972242 (-) 732 WP_032613955.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LA332_RS23720 (LA332_23720) endA 4972354..4973061 (-) 708 WP_032613924.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35677.89 Da        Isoelectric Point: 6.8724

>NTDB_id=606911 LA332_RS23690 WP_032613932.1 4967671..4968648(+) (pilT) [Leclercia adecarboxylata strain FDAARGOS_1505]
MNMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMELAPFPGPDVVMLLKSWLSDEQQGAWCAQGQVDFAVTLESHRLRAS
AFAHTQGCSLALRLLPEQCPQLRTLGTPQAIPELLTRDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCNAFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLQAVLAQKLERDNQQGRVALFELLINTPAAANLIREGKTYQLPGVIQTGLQAGMQNFEQSRAERK
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=606911 LA332_RS23690 WP_032613932.1 4967671..4968648(+) (pilT) [Leclercia adecarboxylata strain FDAARGOS_1505]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGACGACGAGGAAAGATGGAATTGGCGCCTTTTCCGGGACCGGATGTGGTGATGCTGCTGAAAAGCTGGCTCAGCG
ATGAACAACAGGGGGCATGGTGTGCTCAGGGGCAGGTCGATTTTGCCGTCACTCTGGAGAGCCACCGGTTGCGGGCCAGC
GCCTTCGCCCATACGCAAGGGTGTTCGCTGGCGCTCCGGCTGCTGCCGGAACAGTGTCCGCAATTGCGTACCCTAGGTAC
GCCGCAGGCGATCCCCGAACTGCTGACCAGGGATAACGGCCTGATTCTGGTGACCGGTGCCACCGGCAGCGGCAAGTCCA
CCACGCTTGCGGCAATGGTCGACTTTCTTAATCACCACAGCGACGGACATATCCTGACCCTGGAAGATCCGGTGGAGTTT
ATCCATCAGAGCAAACGTTGCCTGGTGCAGCAGCGGGAGATTGGCCTGCACTGCAACGCCTTTGCCGATGCGCTGCGGGC
GGCATTGCGGGAAGATCCCGATGTGATCCTGTTAGGCGAACTGCGCGATAGCGAAACGATCCGCCTTGCGCTTACTGCCG
CAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACCCGTGGCGCAGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCGGAGGAAAAAGAGCCGGTACGCAAGCAACTGGCCGGCAGTTTGCAGGCGGTGCTGGCACAAAAACTCGAAAGGGA
CAACCAGCAGGGGCGCGTGGCGCTGTTTGAGTTACTCATTAACACCCCAGCTGCGGCGAATTTGATTCGTGAAGGCAAAA
CCTATCAACTGCCTGGCGTGATCCAGACCGGGCTGCAGGCAGGGATGCAGAACTTCGAGCAGAGCCGTGCTGAACGCAAG
GCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Legionella pneumophila strain Lp02

47.826

99.077

0.474
  pilT Legionella pneumophila strain ERS1305867

47.826

99.077

0.474
  pilT Neisseria meningitidis 8013

46.951

100

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.538

0.471
  pilT Acinetobacter baylyi ADP1

46.789

100

0.471
  pilT Acinetobacter baumannii strain A118

47.649

98.154

0.468
  pilT Acinetobacter baumannii D1279779

47.649

98.154

0.468
  pilT Acinetobacter nosocomialis M2

47.649

98.154

0.468
  pilT Neisseria gonorrhoeae MS11

47.188

98.462

0.465
  pilT Pseudomonas aeruginosa PAK

47.634

97.538

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.631

96.615

0.422
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.385
  pilU Acinetobacter baylyi ADP1

36.42

99.692

0.363