Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K9N54_RS14265 Genome accession   NZ_CP083361
Coordinates   2966255..2967295 (-) Length   346 a.a.
NCBI ID   WP_005461712.1    Uniprot ID   Q87LJ7
Organism   Vibrio parahaemolyticus strain 20-082E4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2961255..2972295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9N54_RS14235 (K9N54_14120) endA 2961389..2962084 (+) 696 WP_005493878.1 deoxyribonuclease I -
  K9N54_RS14240 (K9N54_14125) rsmE 2962209..2962940 (+) 732 WP_005461719.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K9N54_RS14245 (K9N54_14130) gshB 2962955..2963905 (+) 951 WP_005425823.1 glutathione synthase -
  K9N54_RS14250 (K9N54_14135) - 2964019..2964582 (+) 564 WP_025792786.1 YqgE/AlgH family protein -
  K9N54_RS14255 (K9N54_14140) ruvX 2964631..2965056 (+) 426 WP_005461715.1 Holliday junction resolvase RuvX -
  K9N54_RS14260 (K9N54_14145) pilU 2965118..2966224 (-) 1107 WP_005461714.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K9N54_RS14265 (K9N54_14150) pilT 2966255..2967295 (-) 1041 WP_005461712.1 type IV pilus twitching motility protein PilT Machinery gene
  K9N54_RS14270 (K9N54_14155) - 2967324..2968037 (+) 714 WP_021450047.1 YggS family pyridoxal phosphate-dependent enzyme -
  K9N54_RS14275 (K9N54_14160) proC 2968157..2968975 (+) 819 WP_025792783.1 pyrroline-5-carboxylate reductase -
  K9N54_RS14280 (K9N54_14165) - 2969028..2969585 (+) 558 WP_005482440.1 YggT family protein -
  K9N54_RS14285 (K9N54_14170) yggU 2969585..2969875 (+) 291 WP_005482461.1 DUF167 family protein YggU -
  K9N54_RS14290 (K9N54_14175) - 2970003..2970434 (+) 432 WP_025792781.1 DUF4426 domain-containing protein -
  K9N54_RS14295 (K9N54_14180) - 2970552..2971154 (+) 603 WP_025792780.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38562.05 Da        Isoelectric Point: 6.5039

>NTDB_id=605383 K9N54_RS14265 WP_005461712.1 2966255..2967295(-) (pilT) [Vibrio parahaemolyticus strain 20-082E4]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=605383 K9N54_RS14265 WP_005461712.1 2966255..2967295(-) (pilT) [Vibrio parahaemolyticus strain 20-082E4]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAACTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCCCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCAGTGTTCCGTACCATCCCAAGTAGCATCCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATCGCGAATGCTGAAAAAGGTTTGGTTTTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACAACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCATATCCTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAGTGTTTGATCAACCAACGTGAGGTTCACCGTGATACCCATAGCTTCCAAAA
CGCGCTACGCAGCGCATTGCGTGAAGACCCAGATGTGATCCTTGTCGGTGAGATGCGTGACAAAGAAACCATCAGCCTAG
CGTTAACGGCGGCGGAAACGGGTCACCTAGTTTTCGGTACGTTGCACACCAGTTCAGCAGCAAAAACCATCGACCGTATC
ATCGATGTGTTCCCTGGTAGTGACAAAGACATGGTGCGTTCCATGTTGTCTGAATCCCTTCGTTCAGTTATCGCGCAAAA
ACTGTTGAAACGTAATGGCGGTGGTCGTATCGCTTGTCACGAAATCATGATGGCAACGCCCGCGATTCGTAACTTGATTC
GCGAAGACAAAGTCGCGCAGATGTACTCAATCATTCAAACGGGTGCCGCGCATGGAATGCAAACCATGGAACAAAATGCC
CGTCAGTTGATGGCGCAAGGTATGGTTTCGCGCGAAGAAGTCGACAGCAAGATTGGGCTTGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87LJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

87.246

99.711

0.87
  pilT Vibrio cholerae strain A1552

87.246

99.711

0.87
  pilT Acinetobacter baumannii D1279779

72.727

95.376

0.694
  pilT Acinetobacter baumannii strain A118

72.727

95.376

0.694
  pilT Acinetobacter nosocomialis M2

72.424

95.376

0.691
  pilT Acinetobacter baylyi ADP1

71.515

95.376

0.682
  pilT Pseudomonas aeruginosa PAK

69.733

97.399

0.679
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.399

0.676
  pilT Legionella pneumophila strain Lp02

68.278

95.665

0.653
  pilT Legionella pneumophila strain ERS1305867

68.278

95.665

0.653
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.145

91.908

0.488
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.018

96.532

0.396
  pilU Acinetobacter baylyi ADP1

39.375

92.486

0.364