Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6991_RS03730 Genome accession   NZ_CP082929
Coordinates   777866..778846 (+) Length   326 a.a.
NCBI ID   WP_023255921.1    Uniprot ID   A0A5I3ED36
Organism   Salmonella enterica subsp. enterica serovar London isolate N18-04674     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772866..783846
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6991_RS03700 (K6991_03705) - 773443..774450 (+) 1008 WP_000252197.1 DUF1202 domain-containing protein -
  K6991_RS03705 (K6991_03710) hemW 774542..775678 (-) 1137 WP_001096532.1 radical SAM family heme chaperone HemW -
  K6991_RS03710 (K6991_03715) - 775671..776264 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  K6991_RS03715 (K6991_03720) yggU 776272..776562 (-) 291 WP_023255920.1 DUF167 family protein YggU -
  K6991_RS03720 (K6991_03725) - 776559..777125 (-) 567 WP_001094848.1 YggT family protein -
  K6991_RS03725 (K6991_03730) - 777144..777848 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6991_RS03730 (K6991_03735) pilT 777866..778846 (+) 981 WP_023255921.1 type IV pilus twitching motility protein PilT Machinery gene
  K6991_RS03735 (K6991_03740) - 778978..779664 (+) 687 WP_000098322.1 IclR family transcriptional regulator -
  K6991_RS03740 (K6991_03745) ruvX 779711..780127 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  K6991_RS03745 (K6991_03750) - 780127..780690 (-) 564 WP_020437849.1 YqgE/AlgH family protein -
  K6991_RS03750 (K6991_03755) gshB 780906..781853 (-) 948 WP_000593248.1 glutathione synthase -
  K6991_RS03755 (K6991_03760) rsmE 781873..782604 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6991_RS03760 (K6991_03765) endA 782681..783388 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36123.35 Da        Isoelectric Point: 8.0973

>NTDB_id=603679 K6991_RS03730 WP_023255921.1 777866..778846(+) (pilT) [Salmonella enterica subsp. enterica serovar London isolate N18-04674]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAKIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYSSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=603679 K6991_RS03730 WP_023255921.1 777866..778846(+) (pilT) [Salmonella enterica subsp. enterica serovar London isolate N18-04674]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCA
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGCTGTCCTGATCTGGCGAAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACTCCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5I3ED36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

35.952

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362