Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8O89_RS15035 Genome accession   NZ_CP082852
Coordinates   3367686..3368720 (+) Length   344 a.a.
NCBI ID   WP_019233672.1    Uniprot ID   A0AAX0WV13
Organism   Legionella anisa strain FDAARGOS_1480     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3362686..3373720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O89_RS15000 (K8O89_14970) - 3362751..3362978 (-) 228 WP_127908245.1 hypothetical protein -
  K8O89_RS15005 (K8O89_14975) - 3363127..3364650 (-) 1524 WP_019233677.1 hypothetical protein -
  K8O89_RS15010 (K8O89_14980) - 3364979..3365260 (-) 282 WP_019233676.1 hypothetical protein -
  K8O89_RS15015 (K8O89_14985) - 3365486..3365623 (+) 138 WP_083028154.1 hypothetical protein -
  K8O89_RS15020 (K8O89_14990) - 3365595..3366161 (-) 567 WP_019233675.1 DUF2845 domain-containing protein -
  K8O89_RS15025 (K8O89_14995) - 3366208..3366783 (-) 576 WP_019233674.1 YggT family protein -
  K8O89_RS15030 (K8O89_15000) - 3366841..3367527 (-) 687 WP_019233673.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O89_RS15035 (K8O89_15005) pilT 3367686..3368720 (+) 1035 WP_019233672.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O89_RS15040 (K8O89_15010) rph 3368865..3369572 (-) 708 WP_019233671.1 ribonuclease PH -
  K8O89_RS15045 (K8O89_15015) - 3369998..3370864 (+) 867 WP_019233670.1 YicC/YloC family endoribonuclease -
  K8O89_RS15050 (K8O89_15020) gmk 3370870..3371499 (+) 630 WP_085956448.1 guanylate kinase -
  K8O89_RS15055 (K8O89_15025) rpoZ 3371531..3371734 (+) 204 WP_010653170.1 DNA-directed RNA polymerase subunit omega -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38380.35 Da        Isoelectric Point: 6.4195

>NTDB_id=603061 K8O89_RS15035 WP_019233672.1 3367686..3368720(+) (pilT) [Legionella anisa strain FDAARGOS_1480]
MDIAELLAFSVKNKSSDLHLSAGLPPMIRVDGDLRKINLPALEHKEVIKIIYDVMNDRQRKEFEEFLETDFSFEIPNLAR
FRVNVFNQLRGAAAVFRTIPSEILSMDDLGLPAIFEEMASYPKGLVLITGPTGSGKSTTLAAMIDYINSNRYDHILTVED
PIEFVHQNKKCLLNQREVHRDTLSFNAALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTNSATKTINRI
IDVFPAEEKAMVRSMLSESLQAVVAQTLLKKSGGGRIAALEIMICTGAIRNLIREDKVAQMYSSIQTGQAKGMQTLDQHL
TELVKKNMISTHTARDVAIDKTIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=603061 K8O89_RS15035 WP_019233672.1 3367686..3368720(+) (pilT) [Legionella anisa strain FDAARGOS_1480]
ATGGATATAGCAGAACTATTAGCGTTTTCAGTAAAAAATAAATCATCAGATTTGCATCTTTCTGCAGGATTACCCCCAAT
GATTCGTGTGGATGGGGATTTGCGCAAAATCAATTTACCTGCCCTGGAGCATAAAGAGGTGATAAAAATCATCTATGATG
TGATGAATGACCGGCAAAGGAAAGAATTTGAAGAATTTTTGGAAACCGATTTTTCTTTTGAAATACCCAATCTGGCTCGT
TTTAGGGTGAACGTATTTAATCAATTACGTGGGGCAGCGGCTGTTTTTCGTACTATTCCTTCTGAAATTTTAAGTATGGA
TGATTTAGGATTACCGGCTATTTTTGAAGAAATGGCTAGTTATCCTAAAGGATTGGTACTCATCACTGGTCCAACAGGAT
CAGGAAAAAGCACGACTTTGGCTGCTATGATCGATTACATCAACTCAAATCGTTATGATCACATTTTAACTGTGGAAGAT
CCTATTGAGTTTGTACATCAAAATAAAAAATGTTTGTTGAATCAACGCGAGGTTCATCGAGATACGCTTAGTTTTAATGC
TGCTTTGCGTTCCGCATTACGTGAGGATCCTGATGTGATTTTGGTGGGGGAGCTACGCGATCTGGAAACAATCCGTTTAG
CAATGACCGCTGCGGAAACCGGGCATTTAGTATTTGGCACCTTACATACTAATTCTGCAACCAAAACCATCAATAGGATT
ATTGATGTGTTCCCAGCTGAGGAAAAAGCCATGGTGCGTTCGATGTTATCTGAATCACTGCAAGCCGTAGTAGCGCAAAC
GTTATTGAAAAAAAGTGGTGGTGGGCGAATTGCTGCTTTGGAAATTATGATTTGTACCGGTGCGATACGAAATTTAATTC
GTGAAGATAAAGTGGCACAAATGTATTCTTCCATCCAAACTGGTCAGGCAAAGGGTATGCAAACTTTGGATCAGCATTTA
ACTGAGCTGGTGAAAAAAAATATGATTTCTACTCATACAGCACGTGATGTGGCTATTGATAAAACCATATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

88.081

100

0.881
  pilT Legionella pneumophila strain ERS1305867

88.081

100

0.881
  pilT Acinetobacter baumannii D1279779

76.923

98.256

0.756
  pilT Acinetobacter baylyi ADP1

76.923

98.256

0.756
  pilT Acinetobacter baumannii strain A118

76.923

98.256

0.756
  pilT Acinetobacter nosocomialis M2

76.923

98.256

0.756
  pilT Pseudomonas aeruginosa PAK

75.148

98.256

0.738
  pilT Pseudomonas stutzeri DSM 10701

74.852

98.256

0.735
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.997

95.64

0.66
  pilT Vibrio cholerae strain A1552

68.997

95.64

0.66
  pilT Neisseria meningitidis 8013

65.774

97.674

0.642
  pilT Neisseria gonorrhoeae MS11

65.476

97.674

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.759

96.512

0.384