Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6745_RS13010 Genome accession   NZ_CP082303
Coordinates   2812240..2813346 (-) Length   368 a.a.
NCBI ID   WP_025768829.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain FJV2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2807240..2818346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6745_RS12980 (K6745_12895) - 2807873..2808370 (+) 498 WP_005381025.1 SprT family zinc-dependent metalloprotease -
  K6745_RS12985 (K6745_12900) endA 2808521..2809216 (+) 696 WP_025768831.1 deoxyribonuclease I -
  K6745_RS12990 (K6745_12905) rsmE 2809330..2810061 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6745_RS12995 (K6745_12910) gshB 2810075..2811025 (+) 951 WP_005381021.1 glutathione synthase -
  K6745_RS13000 (K6745_12915) - 2811154..2811717 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  K6745_RS13005 (K6745_12920) ruvX 2811753..2812178 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  K6745_RS13010 (K6745_12925) pilU 2812240..2813346 (-) 1107 WP_025768829.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6745_RS13015 (K6745_12930) pilT 2813377..2814417 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  K6745_RS13020 (K6745_12935) - 2814446..2815147 (+) 702 WP_042521292.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6745_RS13025 (K6745_12940) proC 2815307..2816125 (+) 819 WP_042521294.1 pyrroline-5-carboxylate reductase -
  K6745_RS13030 (K6745_12945) - 2816178..2816735 (+) 558 WP_005381012.1 YggT family protein -
  K6745_RS13035 (K6745_12950) yggU 2816735..2817025 (+) 291 WP_005381011.1 DUF167 family protein YggU -
  K6745_RS13040 (K6745_12955) - 2817154..2817585 (+) 432 WP_042521296.1 DUF4426 domain-containing protein -
  K6745_RS13045 (K6745_12960) - 2817704..2818306 (+) 603 WP_213916736.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41284.45 Da        Isoelectric Point: 6.5943

>NTDB_id=601149 K6745_RS13010 WP_025768829.1 2812240..2813346(-) (pilU) [Vibrio alginolyticus strain FJV2]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKMQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=601149 K6745_RS13010 WP_025768829.1 2812240..2813346(-) (pilU) [Vibrio alginolyticus strain FJV2]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGCGATGTGGCTGCATTACTCGACAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGTTCGGGTA
AATCGACCACTATGGCGGCGATGACAGGTTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAATAATGCCAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTCTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCTTGATGTT
GAAAATGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

58.405

95.38

0.557
  pilU Acinetobacter baylyi ADP1

54.545

95.652

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361