Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5L94_RS02435 Genome accession   NZ_CP082270
Coordinates   526724..527851 (-) Length   375 a.a.
NCBI ID   WP_004154068.1    Uniprot ID   -
Organism   Stenotrophomonas sp. DFS-20110405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 521724..532851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5L94_RS02415 (K5L94_02410) - 522154..523143 (+) 990 WP_049458252.1 fumarylacetoacetate hydrolase family protein -
  K5L94_RS02420 (K5L94_02415) maiA 523158..523826 (+) 669 WP_053448696.1 maleylacetoacetate isomerase -
  K5L94_RS02425 (K5L94_02420) - 523876..525336 (-) 1461 WP_053448697.1 UdgX family uracil-DNA binding protein -
  K5L94_RS02430 (K5L94_02425) - 525317..526600 (-) 1284 WP_053448698.1 putative DNA modification/repair radical SAM protein -
  K5L94_RS02435 (K5L94_02430) pilU 526724..527851 (-) 1128 WP_004154068.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5L94_RS02440 (K5L94_02435) - 527973..528338 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  K5L94_RS02445 (K5L94_02440) - 528335..529219 (+) 885 WP_274511938.1 hypothetical protein -
  K5L94_RS02450 (K5L94_02445) - 529543..529779 (+) 237 WP_004154075.1 YdcH family protein -
  K5L94_RS02455 (K5L94_02450) - 530081..531451 (+) 1371 WP_053448700.1 pyridoxal-phosphate dependent enzyme -
  K5L94_RS02460 (K5L94_02455) - 531585..532778 (+) 1194 WP_274511939.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41368.67 Da        Isoelectric Point: 6.3738

>NTDB_id=600660 K5L94_RS02435 WP_004154068.1 526724..527851(-) (pilU) [Stenotrophomonas sp. DFS-20110405]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGVIDQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=600660 K5L94_RS02435 WP_004154068.1 526724..527851(-) (pilU) [Stenotrophomonas sp. DFS-20110405]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAATACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGATGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGGGGCGAGGTCGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCATATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCTTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCCCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGTCGCCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAAGAAGGTATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCGTCATCGATCAGGAGGAGGCGCTGCGCGCGGCCGACTCACGCGATGGCCTTGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAACACGACCCGTACGCGGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baumannii strain A118

42.012

90.133

0.379
  pilT Acinetobacter nosocomialis M2

42.012

90.133

0.379
  pilT Acinetobacter baumannii D1279779

42.012

90.133

0.379
  pilT Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Vibrio cholerae strain A1552

41.441

88.8

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.8

0.368
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363