Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K4042_RS17345 Genome accession   NZ_CP082269
Coordinates   3577305..3578297 (-) Length   330 a.a.
NCBI ID   WP_222888820.1    Uniprot ID   -
Organism   Enterobacter sp. C2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3572305..3583297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4042_RS17315 - 3573106..3573615 (+) 510 WP_222888816.1 SprT family zinc-dependent metalloprotease -
  K4042_RS17320 endA 3573702..3574400 (+) 699 WP_144816155.1 deoxyribonuclease I -
  K4042_RS17325 rsmE 3574479..3575210 (+) 732 WP_222888817.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K4042_RS17330 gshB 3575242..3576189 (+) 948 WP_222888818.1 glutathione synthase -
  K4042_RS17335 - 3576323..3576886 (+) 564 WP_222888819.1 YqgE/AlgH family protein -
  K4042_RS17340 ruvX 3576886..3577302 (+) 417 WP_144816164.1 Holliday junction resolvase RuvX -
  K4042_RS17345 pilT 3577305..3578297 (-) 993 WP_222888820.1 type IV pilus twitching motility protein PilT Machinery gene
  K4042_RS17350 - 3578317..3579021 (+) 705 WP_222888821.1 YggS family pyridoxal phosphate-dependent enzyme -
  K4042_RS17355 - 3579040..3579606 (+) 567 WP_144816171.1 YggT family protein -
  K4042_RS17360 - 3579646..3580239 (+) 594 WP_222888822.1 XTP/dITP diphosphatase -
  K4042_RS17365 hemW 3580232..3581368 (+) 1137 WP_222888823.1 radical SAM family heme chaperone HemW -
  K4042_RS17370 djlA 3581418..3582236 (-) 819 WP_144816177.1 co-chaperone DjlA -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 36010.09 Da        Isoelectric Point: 6.0775

>NTDB_id=600653 K4042_RS17345 WP_222888820.1 3577305..3578297(-) (pilT) [Enterobacter sp. C2]
MDMEDIVALSVKHNVSDLHLCSAWPPRWRRQGKLEDAPFPPPDIEAILRDTLTDEQQGEWWANRQVDFALALGSQQRLRA
SAFMHHHGISLALRLLPTTCPKLYALDTPPALEQILACDSGLVLVTGATGSGKSTTLAAMVDYLNHRLAGHILTLEDPVE
FVHQGAECLIEQREIGQHCPSFADGLRAALREDPDVILIGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLIDT
FPAEEKAWVRSQLAGSLQAVLAQKLEADKQGGRVALFELLVTTPAVANLIRDGKTHQLSGLMQTGQQAGMQTFSQSRQQR
VAAGRLAEKG

Nucleotide


Download         Length: 993 bp        

>NTDB_id=600653 K4042_RS17345 WP_222888820.1 3577305..3578297(-) (pilT) [Enterobacter sp. C2]
ATGGATATGGAAGACATAGTGGCCCTTAGTGTAAAGCATAACGTTTCGGATCTACACCTGTGCAGCGCCTGGCCGCCGCG
CTGGCGCAGGCAGGGTAAGCTTGAAGATGCCCCGTTTCCGCCGCCGGATATCGAGGCGATCCTCCGCGACACCCTGACGG
ACGAGCAGCAGGGGGAGTGGTGGGCCAACCGGCAGGTAGACTTTGCCCTTGCGCTGGGGAGCCAGCAGCGGCTGCGGGCC
AGCGCCTTTATGCACCATCACGGTATTTCCCTGGCGCTGCGCCTGCTACCCACGACGTGTCCGAAGCTCTACGCTTTGGA
TACGCCGCCGGCGCTGGAGCAGATCCTGGCCTGCGACAGCGGGCTGGTGCTGGTCACCGGCGCAACCGGCAGCGGTAAAT
CCACCACCCTTGCGGCGATGGTCGACTATCTCAACCACCGCCTGGCAGGGCACATTCTCACCCTGGAGGATCCGGTGGAG
TTTGTTCATCAGGGGGCGGAGTGCCTGATCGAGCAGCGGGAGATAGGCCAGCACTGCCCGTCGTTTGCTGACGGCCTGCG
CGCCGCGCTGCGTGAAGATCCAGACGTCATTTTAATCGGCGAGCTGCGGGACAGCGAGACCATTCGTCTGGCGCTGACCG
CTGCCGAGACCGGGCATCTGGTTTTGGCTACGCTGCACACTCGGGGGGCGGCCCAGGCGGTAGAGCGGCTGATCGATACC
TTTCCGGCGGAGGAGAAAGCCTGGGTGCGTAGCCAGCTGGCGGGCAGCCTGCAGGCGGTGCTGGCGCAAAAGCTGGAGGC
CGATAAGCAGGGCGGACGCGTGGCGCTGTTTGAGCTGCTGGTTACCACCCCGGCGGTAGCGAACCTGATCCGCGATGGCA
AAACCCACCAGCTTTCGGGCCTGATGCAGACCGGACAGCAGGCGGGGATGCAGACTTTCAGCCAGAGCCGCCAGCAGCGG
GTTGCCGCCGGGCGGCTGGCAGAGAAGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(69-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

99.091

0.5
  pilT Vibrio cholerae strain A1552

50.459

99.091

0.5
  pilT Neisseria meningitidis 8013

48.943

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.64

100

0.488
  pilT Legionella pneumophila strain Lp02

48.742

96.364

0.47
  pilT Legionella pneumophila strain ERS1305867

48.742

96.364

0.47
  pilT Acinetobacter baumannii strain A118

46.061

100

0.461
  pilT Acinetobacter baumannii D1279779

46.061

100

0.461
  pilT Pseudomonas stutzeri DSM 10701

46.061

100

0.461
  pilT Acinetobacter baylyi ADP1

46.483

99.091

0.461
  pilT Acinetobacter nosocomialis M2

45.758

100

0.458
  pilT Pseudomonas aeruginosa PAK

45.345

100

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.969

96.364

0.433
  pilU Vibrio cholerae strain A1552

41.25

96.97

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.397
  pilU Acinetobacter baylyi ADP1

38.006

97.273

0.37