Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5R88_RS19015 Genome accession   NZ_CP081943
Coordinates   4173153..4174187 (-) Length   344 a.a.
NCBI ID   WP_226298191.1    Uniprot ID   -
Organism   Pseudomonas sp. MM213     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4168153..4179187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5R88_RS18985 (K5R88_18880) ruvX 4168300..4168737 (+) 438 WP_007945492.1 Holliday junction resolvase RuvX -
  K5R88_RS18990 (K5R88_18885) pyrR 4168820..4169323 (+) 504 WP_008026190.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  K5R88_RS18995 (K5R88_18890) - 4169351..4170355 (+) 1005 WP_008026189.1 aspartate carbamoyltransferase catalytic subunit -
  K5R88_RS19000 (K5R88_18895) - 4170352..4171623 (+) 1272 WP_008026186.1 dihydroorotase -
  K5R88_RS19005 (K5R88_18900) - 4171718..4172149 (-) 432 WP_008026184.1 TM2 domain-containing protein -
  K5R88_RS19010 (K5R88_18905) - 4172400..4173020 (+) 621 WP_008036675.1 C40 family peptidase -
  K5R88_RS19015 (K5R88_18910) pilT 4173153..4174187 (-) 1035 WP_226298191.1 type IV pilus twitching motility protein PilT Machinery gene
  K5R88_RS19020 (K5R88_18915) - 4174245..4174931 (+) 687 WP_008036679.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5R88_RS19025 (K5R88_18920) proC 4174968..4175786 (+) 819 WP_207284875.1 pyrroline-5-carboxylate reductase -
  K5R88_RS19030 (K5R88_18925) - 4175811..4176398 (+) 588 WP_192227547.1 YggT family protein -
  K5R88_RS19035 (K5R88_18930) - 4176601..4177740 (+) 1140 WP_008026171.1 homoserine O-acetyltransferase -
  K5R88_RS19040 (K5R88_18935) metW 4177748..4178368 (+) 621 WP_008026169.1 methionine biosynthesis protein MetW -
  K5R88_RS19045 (K5R88_18940) - 4178395..4178829 (+) 435 WP_226298192.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38094.68 Da        Isoelectric Point: 6.8287

>NTDB_id=598593 K5R88_RS19015 WP_226298191.1 4173153..4174187(-) (pilT) [Pseudomonas sp. MM213]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVQELVYDIMNDTQRVDFEKHLETDFSFDVPGVAR
FRVNAFNQSRGAGAVFRTIPSKVLSMEDLGMGEVFRKITDSPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGNLGMQTLDMCL
KELVTKGLISREHARDKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=598593 K5R88_RS19015 WP_226298191.1 4173153..4174187(-) (pilT) [Pseudomonas sp. MM213]
ATGGATATCACTGAGCTGCTGGCGTTCAGCGCCAAACAAGGAGCGTCCGACTTGCACCTGTCGGCAGGCCTGCCACCGAT
GATTCGCGTCGACGGCGATGTGCGGCGCATCAATCTGCCAGCCCTGGATCACAAGGAAGTTCAGGAGCTGGTCTACGACA
TCATGAACGACACCCAGCGGGTGGATTTCGAGAAACACCTGGAAACCGACTTTTCCTTTGATGTGCCCGGCGTTGCGCGT
TTCCGGGTCAATGCGTTCAACCAGAGCCGTGGCGCCGGCGCGGTGTTCCGAACGATTCCGTCGAAGGTCCTGAGCATGGA
AGACCTCGGGATGGGGGAAGTGTTTCGCAAGATTACCGACTCCCCCCGTGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGACTACCTGAACAACCATCGTCATCACCACATCCTGACCATTGAAGAC
CCGATCGAATTCGTCCACGAATCACGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGCGATACCCGTAGCTTCGCCAC
CGCCTTGCGCTCGGCGTTGCGGGAGGATCCGGATGTGATTCTGGTGGGCGAGATGCGTGATCTGGAGACTATTCGCCTGG
CGCTGACGGCTGCCGAGACTGGCCATCTGGTGTTTGGCACGTTGCACACCACGTCGGCGGCGAAAACCATCGACCGGATC
GTGGATGTGTTTCCGGGGGAAGAGAAGTCGATGGTGCGTTCGATGTTGTCCGAGTCGCTGCTGGCGGTGGTATCGCAGAC
GCTGGTGAAGAAGATCGGCGGCGGGCGGATCGCGGCGCACGAAATCATGCTGGGAACATCGGCGATCCGTAACCTGATCC
GTGAGGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGCGGGAATCTGGGGATGCAGACGCTGGATATGTGCTTG
AAGGAGTTGGTGACCAAGGGCTTGATCAGCCGCGAGCATGCGCGGGATAAGGCGCGGTCGCCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

86.628

100

0.866
  pilT Pseudomonas aeruginosa PAK

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.093

100

0.721
  pilT Legionella pneumophila strain ERS1305867

72.093

100

0.721
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404