Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M495_RS20565 Genome accession   NC_021741
Coordinates   4405513..4406535 (-) Length   340 a.a.
NCBI ID   WP_020828600.1    Uniprot ID   -
Organism   Serratia liquefaciens ATCC 27592     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4400513..4411535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M495_RS20535 (M495_20960) - 4401137..4401649 (+) 513 WP_041414952.1 SprT family zinc-dependent metalloprotease -
  M495_RS20540 (M495_20965) endA 4401801..4402496 (+) 696 WP_020828595.1 deoxyribonuclease I -
  M495_RS20545 (M495_20970) rsmE 4402683..4403414 (+) 732 WP_020828596.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M495_RS20550 (M495_20975) gshB 4403425..4404375 (+) 951 WP_020828597.1 glutathione synthase -
  M495_RS20555 (M495_20980) - 4404531..4405094 (+) 564 WP_041414955.1 YqgE/AlgH family protein -
  M495_RS20560 (M495_20985) ruvX 4405094..4405516 (+) 423 WP_020828599.1 Holliday junction resolvase RuvX -
  M495_RS20565 (M495_20990) pilT 4405513..4406535 (-) 1023 WP_020828600.1 type IV pilus twitching motility protein PilT Machinery gene
  M495_RS20570 (M495_20995) - 4406556..4407263 (+) 708 WP_020828601.1 YggS family pyridoxal phosphate-dependent enzyme -
  M495_RS20575 (M495_21000) proC 4407284..4408105 (+) 822 WP_020828602.1 pyrroline-5-carboxylate reductase -
  M495_RS20580 (M495_21005) - 4408137..4408691 (+) 555 WP_020828603.1 YggT family protein -
  M495_RS20585 (M495_21010) yggU 4408688..4408978 (+) 291 WP_020828604.1 DUF167 family protein YggU -
  M495_RS20590 (M495_21015) - 4408996..4409589 (+) 594 WP_020828605.1 XTP/dITP diphosphatase -
  M495_RS20595 (M495_21020) hemW 4409582..4410721 (+) 1140 WP_020828606.1 radical SAM family heme chaperone HemW -
  M495_RS20600 (M495_21025) - 4410758..4411006 (-) 249 WP_020828607.1 DUF2442 domain-containing protein -
  M495_RS20605 (M495_21030) - 4410990..4411232 (-) 243 WP_020828608.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36764.19 Da        Isoelectric Point: 6.7738

>NTDB_id=59853 M495_RS20565 WP_020828600.1 4405513..4406535(-) (pilT) [Serratia liquefaciens ATCC 27592]
MDIGEFVALSVKHNASDLHLCTGHQPMLRIDGELQPLAGAEKLTETKMHRLCNALLQPQQRQQLQQQGQLDLALTLAGDI
RLRANFFLQSLGVSLALRLIAGECPTLAALAAPDIIPALVRREDGLILVTGATGSGKSTTLAAMIDDINQHQRRHILTLE
DPIEFIHRSRHSLIQQRELGRDSHSFDDALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSATQAVDR
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLLSRPGGGRVAIFEVLTATAAVSSMIREGKTHQLASVLQTGGQSGMQTFEQG
LQQRICAGLLGGAEGTLPRD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=59853 M495_RS20565 WP_020828600.1 4405513..4406535(-) (pilT) [Serratia liquefaciens ATCC 27592]
ATGGATATCGGTGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTACGGGGCATCAGCCGAT
GTTGCGCATCGATGGCGAATTACAGCCGCTGGCAGGTGCGGAAAAACTGACCGAGACCAAGATGCATCGCTTGTGCAACG
CGCTGCTGCAACCCCAGCAACGGCAGCAATTGCAACAGCAGGGGCAGCTTGATCTGGCGCTGACGCTTGCGGGCGATATT
CGGCTGCGCGCTAATTTCTTTCTGCAAAGCCTCGGGGTTTCGCTTGCCTTGAGGCTGATTGCCGGCGAGTGCCCGACGCT
GGCGGCGTTGGCAGCACCAGACATCATTCCGGCCCTGGTGCGCAGGGAAGATGGCCTGATTTTGGTGACCGGCGCGACCG
GCAGCGGTAAATCCACCACCTTGGCGGCAATGATTGACGACATCAATCAGCACCAGCGGCGGCATATCCTTACGCTGGAG
GATCCGATCGAATTTATTCACCGCAGTCGACACTCTTTAATTCAGCAGCGCGAATTGGGCCGCGACAGCCACAGCTTTGA
CGACGCATTACGTGCTGCCCTGCGTGAAGATCCAGACGTCATCTTGCTGGGGGAGCTGCGTGACACCACAACCATCAGGC
TGGCGCTTACCGCTGCGGAAACCGGCCATCTGGTACTGGCGACGCTGCATACCCGTAGCGCGACGCAGGCGGTCGATCGG
CTGGTGGATGTTTTCCCGGCGGAAGAAAAGCCCTACGTGCGTGCACAGTTGGCCGGTAGCCTGCAGGCGGTGATTGCACA
GAAACTGTTGAGTCGGCCCGGGGGCGGCAGGGTGGCGATTTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAGCATGA
TCCGCGAGGGCAAAACCCATCAGCTTGCCAGCGTGCTGCAAACCGGTGGGCAGTCCGGCATGCAGACCTTTGAGCAAGGT
CTGCAACAGCGGATCTGTGCGGGATTACTGGGCGGCGCGGAGGGGACTTTGCCAAGGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.593

98.235

0.526
  pilT Vibrio cholerae strain A1552

53.593

98.235

0.526
  pilT Neisseria meningitidis 8013

49.699

97.647

0.485
  pilT Neisseria gonorrhoeae MS11

49.398

97.647

0.482
  pilT Acinetobacter baumannii strain A118

49.394

97.059

0.479
  pilT Acinetobacter nosocomialis M2

49.394

97.059

0.479
  pilT Acinetobacter baumannii D1279779

49.394

97.059

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.353

0.476
  pilT Pseudomonas stutzeri DSM 10701

48.485

97.059

0.471
  pilT Pseudomonas aeruginosa PAK

48.182

97.059

0.468
  pilT Legionella pneumophila strain ERS1305867

48.036

97.353

0.468
  pilT Legionella pneumophila strain Lp02

48.036

97.353

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.894

94.706

0.444
  pilU Acinetobacter baylyi ADP1

39.319

95

0.374
  pilU Pseudomonas stutzeri DSM 10701

38.253

97.647

0.374
  pilU Vibrio cholerae strain A1552

38.226

96.176

0.368

Multiple sequence alignment