Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6T23_RS15400 Genome accession   NZ_CP081870
Coordinates   2930670..2931707 (-) Length   345 a.a.
NCBI ID   WP_238281635.1    Uniprot ID   -
Organism   Rossellomorea marisflavi strain UTDF19-31A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2925670..2936707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6T23_RS15370 (K6T23_15395) - 2925907..2926584 (-) 678 WP_238281623.1 hypothetical protein -
  K6T23_RS15375 (K6T23_15400) - 2926581..2927180 (-) 600 WP_238281625.1 PilN domain-containing protein -
  K6T23_RS15380 (K6T23_15405) pilM 2927177..2928112 (-) 936 WP_238281627.1 type IV pilus biogenesis protein PilM -
  K6T23_RS15385 (K6T23_15410) - 2928127..2928870 (-) 744 WP_238281629.1 prepilin peptidase -
  K6T23_RS15390 (K6T23_15415) - 2928877..2929350 (-) 474 WP_238281631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K6T23_RS15395 (K6T23_15420) - 2929458..2930669 (-) 1212 WP_238281633.1 type II secretion system F family protein -
  K6T23_RS15400 (K6T23_15425) pilT 2930670..2931707 (-) 1038 WP_238281635.1 type IV pilus twitching motility protein PilT Machinery gene
  K6T23_RS15405 (K6T23_15430) - 2931716..2933371 (-) 1656 WP_238281637.1 GspE/PulE family protein -
  K6T23_RS15410 (K6T23_15435) - 2933385..2934782 (-) 1398 WP_238281639.1 VanW family protein -
  K6T23_RS15415 (K6T23_15440) - 2934796..2935569 (-) 774 WP_238281641.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37740.40 Da        Isoelectric Point: 7.6733

>NTDB_id=597927 K6T23_RS15400 WP_238281635.1 2930670..2931707(-) (pilT) [Rossellomorea marisflavi strain UTDF19-31A]
MKDKIDALLRSAYELGASDIHLTVGAPPVFRIHGDLKRYGKDALSPETTEGMAKALIAPPMYTSFEEAGELDFSYGIPGV
SRFRINAFRQRSCVSLAIRVIPTSIPTMEDLFLPHALKGIAGRPQGLFLVTGPTGSGKSTTLASIIDHMNRTMRKHIITL
EDPIEYLHRHGSSIIDQREVGFDTGSFSRGLRSALRQDPDIILVGEMRDLETIHTAITAAETGHLVLATLHTSSAPATIE
RIVDVFPPEQQAQIRVQLASVLTGILSQRLFPTVDRKGRRAATELLLNNAAVANLIRSEKVHQIQNVMQTSRASGMHLMS
DSVQGLYEAGIVSKEMALPYIREGA

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=597927 K6T23_RS15400 WP_238281635.1 2930670..2931707(-) (pilT) [Rossellomorea marisflavi strain UTDF19-31A]
ATGAAAGATAAAATAGATGCGCTCCTCAGAAGCGCGTATGAACTCGGGGCCTCGGACATCCACCTGACCGTCGGGGCGCC
GCCGGTATTCCGCATCCACGGGGACCTGAAACGGTACGGGAAGGATGCCCTCTCCCCGGAAACGACCGAGGGGATGGCCA
AAGCCTTGATCGCCCCGCCCATGTATACGAGCTTTGAAGAAGCAGGCGAACTTGATTTCTCATATGGCATCCCAGGTGTA
TCCCGGTTCAGGATCAATGCTTTCCGGCAGCGCTCCTGCGTGTCCCTGGCCATCCGGGTGATTCCGACATCGATTCCGAC
AATGGAGGACCTGTTCCTTCCCCATGCGCTGAAAGGGATCGCCGGAAGGCCCCAGGGCCTCTTCCTCGTGACGGGACCGA
CGGGCAGCGGGAAATCGACGACCCTAGCTTCCATCATCGATCATATGAACAGGACGATGAGGAAGCATATCATCACCCTG
GAGGACCCCATCGAGTATCTTCACCGCCACGGCAGTTCCATCATCGATCAGCGGGAGGTCGGATTTGATACGGGTTCTTT
TTCCAGAGGATTGAGGAGCGCTCTGAGGCAGGACCCCGACATCATCCTTGTGGGGGAAATGAGGGACCTTGAAACGATCC
ATACGGCCATCACGGCTGCGGAGACGGGTCACCTCGTGCTGGCGACCCTTCATACCTCCAGTGCGCCGGCAACCATCGAG
CGCATCGTGGACGTGTTCCCGCCTGAGCAACAGGCACAGATCCGCGTCCAGCTTGCGTCCGTCCTGACGGGCATCCTTTC
GCAGCGCCTGTTCCCGACCGTGGACCGGAAAGGCCGCCGAGCTGCGACGGAGCTTCTGTTGAATAATGCGGCAGTGGCCA
ACCTCATCCGTTCGGAGAAAGTGCATCAAATCCAAAACGTCATGCAGACGTCGCGGGCTTCGGGCATGCATCTTATGAGC
GACAGCGTCCAGGGACTGTACGAGGCGGGGATCGTCTCCAAGGAAATGGCGCTGCCCTATATCCGGGAGGGGGCGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.395

96.812

0.507
  pilT Pseudomonas stutzeri DSM 10701

47.147

96.522

0.455
  pilT Pseudomonas aeruginosa PAK

47.147

96.522

0.455
  pilT Neisseria meningitidis 8013

46.707

96.812

0.452
  pilT Neisseria gonorrhoeae MS11

46.407

96.812

0.449
  pilT Acinetobacter nosocomialis M2

46.246

96.522

0.446
  pilT Acinetobacter baumannii D1279779

46.246

96.522

0.446
  pilT Acinetobacter baumannii strain A118

46.246

96.522

0.446
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.224

95.942

0.443
  pilT Vibrio cholerae strain A1552

46.224

95.942

0.443
  pilT Legionella pneumophila strain Lp02

45.045

96.522

0.435
  pilT Legionella pneumophila strain ERS1305867

45.045

96.522

0.435
  pilT Acinetobacter baylyi ADP1

44.681

95.362

0.426
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.101

0.409
  pilU Vibrio cholerae strain A1552

40.683

93.333

0.38
  pilU Acinetobacter baylyi ADP1

39.692

94.203

0.374