Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5K93_RS10065 Genome accession   NZ_CP081826
Coordinates   2176911..2178038 (+) Length   375 a.a.
NCBI ID   WP_025878450.1    Uniprot ID   A0A1W1H1T2
Organism   Stenotrophomonas sp. DR822     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171911..2183038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K93_RS10040 (K5K93_10040) - 2171953..2173137 (-) 1185 WP_222390851.1 cystathionine gamma-synthase -
  K5K93_RS10045 (K5K93_10045) - 2173261..2174631 (-) 1371 WP_025878454.1 pyridoxal-phosphate dependent enzyme -
  K5K93_RS10050 (K5K93_10050) - 2174983..2175219 (-) 237 WP_093818802.1 YdcH family protein -
  K5K93_RS10055 (K5K93_10055) - 2175543..2176427 (-) 885 WP_222390853.1 hypothetical protein -
  K5K93_RS10060 (K5K93_10060) - 2176424..2176789 (-) 366 WP_025878451.1 DUF4398 domain-containing protein -
  K5K93_RS10065 (K5K93_10065) pilU 2176911..2178038 (+) 1128 WP_025878450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5K93_RS10070 (K5K93_10070) - 2178169..2179452 (+) 1284 WP_093818801.1 putative DNA modification/repair radical SAM protein -
  K5K93_RS10075 (K5K93_10075) - 2179433..2180905 (+) 1473 WP_222390855.1 UdgX family uracil-DNA binding protein -
  K5K93_RS10080 (K5K93_10080) maiA 2180939..2181607 (-) 669 WP_222390857.1 maleylacetoacetate isomerase -
  K5K93_RS10085 (K5K93_10085) - 2181622..2182611 (-) 990 WP_222390858.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41371.63 Da        Isoelectric Point: 6.3738

>NTDB_id=597593 K5K93_RS10065 WP_025878450.1 2176911..2178038(+) (pilU) [Stenotrophomonas sp. DR822]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGTIDQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=597593 K5K93_RS10065 WP_025878450.1 2176911..2178038(+) (pilU) [Stenotrophomonas sp. DR822]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTATCCGCTCGGCAATACCGGCCTGCCGCCGGGAATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGTGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTCGGGCTCGATACCCATGCCTTCCACAA
TGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCAGAAACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCCCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGGCGCCGCCTGCCGGCCACCGAGGTCCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGTGGCCAGGTGCACGAGATCAAGGCGGCAATGGAGGCTTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGTTGGCCAAGAACGGCACCATCGATCAGGAAGAAGCCCTGCGCGCGGCCGACTCACGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCACGATCCGTATGCGGACTTCTCGGCCGGCGCGCCGAGCATTACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W1H1T2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Vibrio cholerae strain A1552

49.167

96

0.472
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.772

99.467

0.445
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36