Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5F93_RS02095 Genome accession   NZ_CP081490
Coordinates   437009..438043 (+) Length   344 a.a.
NCBI ID   WP_221810408.1    Uniprot ID   -
Organism   Pseudomonas protegens strain PS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432009..443043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5F93_RS02065 (K5F93_02065) metW 432438..433058 (-) 621 WP_011064017.1 methionine biosynthesis protein MetW -
  K5F93_RS02070 (K5F93_02070) - 433066..434205 (-) 1140 WP_221810405.1 homoserine O-acetyltransferase -
  K5F93_RS02075 (K5F93_02075) - 434369..434662 (-) 294 WP_102862081.1 DUF167 domain-containing protein -
  K5F93_RS02080 (K5F93_02080) - 434662..435249 (-) 588 WP_102862080.1 YggT family protein -
  K5F93_RS02085 (K5F93_02085) proC 435274..436092 (-) 819 WP_221810406.1 pyrroline-5-carboxylate reductase -
  K5F93_RS02090 (K5F93_02090) - 436127..436825 (-) 699 WP_221810407.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5F93_RS02095 (K5F93_02095) pilT 437009..438043 (+) 1035 WP_221810408.1 type IV pilus twitching motility protein PilT Machinery gene
  K5F93_RS02100 (K5F93_02100) - 438130..438753 (-) 624 WP_102862076.1 C40 family peptidase -
  K5F93_RS02105 (K5F93_02105) - 439005..439424 (+) 420 WP_102862075.1 NINE protein -
  K5F93_RS02110 (K5F93_02110) - 439552..440823 (-) 1272 WP_221810409.1 dihydroorotase -
  K5F93_RS02115 (K5F93_02115) - 440820..441824 (-) 1005 WP_221810410.1 aspartate carbamoyltransferase catalytic subunit -
  K5F93_RS02120 (K5F93_02120) pyrR 441850..442356 (-) 507 WP_221810411.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  K5F93_RS02125 (K5F93_02125) ruvX 442473..442910 (-) 438 WP_016964942.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37464.29 Da        Isoelectric Point: 7.0816

>NTDB_id=596949 K5F93_RS02095 WP_221810408.1 437009..438043(+) (pilT) [Pseudomonas protegens strain PS1]
MDITELLTLSISRGASDLHLSAGLAPMLRIDGDIRPLDSPALDARQVLSLMHGLMTEPQRQVFASQQDLDFAYELPGVAR
FRVNAFQHVRGAAAVLRLIPSRVQSLEALGLGQVFQQIAEAPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRQQHILTLED
PIEFIHPQKSCLIHQREVPRDTRSFATALRAALREDPDVILLGELRDLESIRLALTAAETGHLVFATLHTASAAKSIDRL
VDVFPGEEKALVRSMLAESLQAVIAQVLLKKVGGGRVAAHEIMLGNAAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISREQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=596949 K5F93_RS02095 WP_221810408.1 437009..438043(+) (pilT) [Pseudomonas protegens strain PS1]
ATGGACATCACCGAACTCCTGACTTTGAGCATCTCCCGGGGCGCTTCCGACCTGCATCTGTCTGCCGGGCTGGCGCCGAT
GTTGCGCATTGATGGCGATATTCGTCCGCTGGACAGCCCGGCCCTGGATGCACGGCAGGTGCTGTCCTTGATGCATGGGC
TGATGACTGAGCCACAGCGGCAGGTGTTCGCCAGCCAGCAGGATCTGGACTTTGCCTACGAACTGCCGGGCGTGGCGCGG
TTCCGGGTCAATGCCTTCCAGCATGTCCGGGGAGCGGCTGCAGTGCTGCGCCTGATCCCGTCACGGGTACAGAGCCTGGA
GGCGTTGGGACTGGGGCAAGTGTTTCAGCAGATTGCCGAAGCGCCCCGGGGGCTGGTGCTGGTCACCGGACCCACCGGTA
GCGGCAAGTCCACCACCCTGGCGGCGATGATCGATCACCTGAACCAGCATCGGCAGCAGCACATCCTCACCCTCGAAGAC
CCCATCGAGTTCATTCATCCGCAGAAAAGTTGCCTGATCCACCAGCGCGAGGTGCCGCGTGATACCCGCAGTTTCGCCAC
CGCCTTGCGTGCGGCGCTGCGGGAGGACCCGGACGTGATCCTGCTGGGAGAGTTGCGCGATCTGGAAAGCATTCGCCTGG
CCCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGCCACCCTGCACACTGCATCCGCGGCCAAGAGCATCGACCGGCTG
GTGGACGTGTTTCCGGGGGAGGAAAAGGCCCTGGTGCGCTCGATGCTGGCCGAGTCGTTGCAGGCGGTCATTGCCCAGGT
GTTGCTGAAGAAAGTGGGCGGTGGGCGGGTGGCGGCTCACGAAATCATGCTGGGCAATGCGGCGATCCGGAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGTGGGGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTGGGGCAAGGGCTGATCAGCCGTGAACAGGCGCGGGAGCAGGCCCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

68.605

100

0.686
  pilT Pseudomonas stutzeri DSM 10701

68.605

100

0.686
  pilT Acinetobacter nosocomialis M2

66.279

100

0.663
  pilT Acinetobacter baumannii D1279779

66.279

100

0.663
  pilT Acinetobacter baumannii strain A118

66.279

100

0.663
  pilT Acinetobacter baylyi ADP1

65.698

100

0.657
  pilT Legionella pneumophila strain Lp02

61.538

98.256

0.605
  pilT Legionella pneumophila strain ERS1305867

61.538

98.256

0.605
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

60.882

98.837

0.602
  pilT Vibrio cholerae strain A1552

60.882

98.837

0.602
  pilT Neisseria meningitidis 8013

59.42

100

0.596
  pilT Neisseria gonorrhoeae MS11

59.13

100

0.593
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

39.13

100

0.392
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387