Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Cf24236_RS03835 Genome accession   NZ_CP081314
Coordinates   789178..790158 (+) Length   326 a.a.
NCBI ID   WP_216526409.1    Uniprot ID   -
Organism   Citrobacter farmeri strain CCRI-24236     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 784178..795158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Cf24236_RS03805 (Cf24236_0761) ansB 784768..785814 (+) 1047 WP_042324112.1 L-asparaginase 2 -
  Cf24236_RS03810 (Cf24236_0762) hemW 785854..786990 (-) 1137 WP_216526411.1 radical SAM family heme chaperone HemW -
  Cf24236_RS03815 (Cf24236_0763) - 786983..787576 (-) 594 WP_216526410.1 XTP/dITP diphosphatase -
  Cf24236_RS03820 (Cf24236_0764) yggU 787584..787874 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  Cf24236_RS03825 (Cf24236_0765) - 787871..788437 (-) 567 WP_195295009.1 YggT family protein -
  Cf24236_RS03830 (Cf24236_0766) - 788456..789160 (-) 705 WP_042324102.1 YggS family pyridoxal phosphate-dependent enzyme -
  Cf24236_RS03835 (Cf24236_0767) pilT 789178..790158 (+) 981 WP_216526409.1 type IV pilus twitching motility protein PilT Machinery gene
  Cf24236_RS03840 (Cf24236_0768) ruvX 790172..790588 (-) 417 WP_061077975.1 Holliday junction resolvase RuvX -
  Cf24236_RS03845 (Cf24236_0769) - 790588..791151 (-) 564 WP_195505253.1 YqgE/AlgH family protein -
  Cf24236_RS03850 (Cf24236_0770) gshB 791342..792289 (-) 948 WP_195295021.1 glutathione synthase -
  Cf24236_RS03855 (Cf24236_0771) rsmE 792302..793033 (-) 732 WP_195505254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Cf24236_RS03860 (Cf24236_0772) endA 793113..793820 (-) 708 WP_216526413.1 deoxyribonuclease I -
  Cf24236_RS03865 (Cf24236_0773) - 793912..794412 (-) 501 WP_042324090.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35522.71 Da        Isoelectric Point: 7.0146

>NTDB_id=595965 Cf24236_RS03835 WP_216526409.1 789178..790158(+) (pilT) [Citrobacter farmeri strain CCRI-24236]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMECAPFTAADITGLLADWLDEGQQAALRQNGQLDFAVSLVGNLRLRA
SAFHQRQGTSLALRLLPTKCPTLETLGTPSVLPELLGSENGLILVTGATGSGKSTTLAAMVSWLNQHVDGHILTLEDPIE
YVYASQRCLIQQREVGLHCASFAAGLRAALREDPDVILLGELRDSETIRLALTAAEMGHLVLATLHTRGAAQAIERLIDS
FTAQEKDPVRSQLAGSLRAVLSQKLESDKQEGRVALFELLINTPAAGNLIREGKSHQLPHIIQTGQQVGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=595965 Cf24236_RS03835 WP_216526409.1 789178..790158(+) (pilT) [Citrobacter farmeri strain CCRI-24236]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGCCGTATGGAATGCGCCCCTTTCACCGCAGCCGACATTACCGGACTGCTCGCGGACTGGCTGGATG
AAGGGCAACAGGCGGCGTTACGGCAAAATGGACAACTGGACTTCGCCGTTTCGCTTGTGGGAAACCTGCGGTTGCGGGCC
AGCGCATTTCATCAGCGACAGGGAACGTCGTTGGCACTGCGACTGCTGCCGACGAAGTGCCCGACGCTGGAAACACTGGG
GACACCCTCCGTGCTGCCTGAACTGCTCGGCAGCGAGAACGGCTTAATTCTGGTGACGGGCGCAACGGGCAGTGGAAAAT
CGACCACCCTGGCGGCGATGGTGTCGTGGCTGAATCAGCATGTCGATGGGCATATTCTGACGCTCGAAGATCCCATTGAG
TATGTCTACGCCAGTCAGCGTTGTCTGATTCAACAGCGTGAGGTGGGATTACACTGCGCTTCGTTTGCCGCAGGGTTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGGGAGCTACGTGACAGCGAAACCATTCGTCTGGCGCTAACGG
CGGCGGAAATGGGGCATCTGGTGTTGGCGACGCTGCATACGCGCGGAGCCGCGCAGGCGATCGAGCGGTTGATCGATTCT
TTTACGGCGCAGGAAAAAGATCCGGTGCGCAGCCAACTGGCTGGCAGTCTGCGTGCCGTGCTGTCGCAAAAACTGGAATC
TGACAAGCAGGAGGGACGCGTTGCGCTATTTGAACTATTGATTAACACCCCGGCGGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCACCAGTTGCCTCATATCATTCAGACCGGGCAGCAGGTGGGGATGATGACGTTTGCCCAGAGCCTGCAACAGCGA
CAGGCGCAGGGAAGGCTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(60-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilU Vibrio cholerae strain A1552

43.226

95.092

0.411
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383