NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	K3169_RS05240	Genome accession	NZ_CP081201
Coordinates	1208602..1209033 (+)	Length	143 a.a.
NCBI ID	WP_231667862.1	Uniprot ID	-
Organism	Pseudomonas phytophila strain ICMP 23753
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1203602..1214033

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K3169_RS05215 (K3169_05175)	yacG	1203768..1203968 (-)	201	WP_027902143.1	DNA gyrase inhibitor YacG	-
K3169_RS05220 (K3169_05180)	coaE	1203965..1204588 (-)	624	WP_263270468.1	dephospho-CoA kinase	-
K3169_RS05225 (K3169_05185)	pilD	1204585..1205457 (-)	873	WP_263270470.1	prepilin peptidase	Machinery gene
K3169_RS05230 (K3169_05190)	pilC	1205460..1206680 (-)	1221	WP_231667864.1	type II secretion system F family protein	Machinery gene
K3169_RS05235 (K3169_05195)	pilB	1206683..1208377 (-)	1695	WP_231667863.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K3169_RS05240 (K3169_05200)	pilA2	1208602..1209033 (+)	432	WP_231667862.1	pilin	Machinery gene
K3169_RS05245 (K3169_05205)	nadC	1209449..1210297 (-)	849	WP_263270475.1	carboxylating nicotinate-nucleotide diphosphorylase	-
K3169_RS05250 (K3169_05210)	-	1210656..1212908 (+)	2253	WP_122708265.1	DUF1631 domain-containing protein	-
K3169_RS05255 (K3169_05215)	ampD	1213152..1213700 (+)	549	WP_122708262.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14547.88 Da Isoelectric Point: 9.0030

                                >NTDB_id=595629 K3169_RS05240 WP_231667862.1 1208602..1209033(+) (pilA2) [Pseudomonas phytophila strain ICMP 23753]

MKKVQKGFTLIELMIVVAIVGILAAVAIPAYQDYTIRAQVAELVALADGAKVAVAEAYQTNGVFPATNAAAGYAGASGTY

TASVAIGAAGIITATSLNAAPVNAAVRNMTFILTPGLGTNNSITWTCLAGTIPVQYRPANCRS

Nucleotide

Download Length: 432 bp

                                >NTDB_id=595629 K3169_RS05240 WP_231667862.1 1208602..1209033(+) (pilA2) [Pseudomonas phytophila strain ICMP 23753]

ATGAAAAAAGTACAAAAAGGTTTCACGCTGATTGAGCTGATGATCGTCGTGGCTATCGTTGGCATCCTTGCTGCGGTGGC

CATCCCCGCATATCAGGACTACACAATCCGCGCTCAGGTGGCAGAGTTGGTGGCGCTCGCGGATGGTGCTAAGGTCGCTG

TTGCTGAGGCTTACCAGACTAATGGTGTGTTTCCAGCCACAAACGCAGCTGCGGGCTACGCTGGCGCGTCCGGTACTTAC

ACTGCCTCTGTAGCGATTGGCGCCGCTGGTATCATTACCGCGACATCGCTCAATGCTGCGCCTGTGAATGCTGCCGTCCG

CAACATGACTTTTATTCTTACGCCTGGACTCGGCACTAATAACAGCATCACTTGGACATGCCTAGCTGGTACTATCCCCG

TTCAGTATCGCCCAGCCAACTGCCGCAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	58.273	97.203	0.566
pilA2	Legionella pneumophila strain ERS1305867	58.273	97.203	0.566
pilA	Ralstonia pseudosolanacearum GMI1000	41.04	100	0.496
pilE	Neisseria gonorrhoeae MS11	42.331	100	0.483
pilE	Neisseria gonorrhoeae strain FA1090	49.231	90.909	0.448
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	33.511	100	0.441
pilA/pilA1	Eikenella corrodens VA1	40.909	100	0.441
pilA	Vibrio campbellii strain DS40M4	39.61	100	0.427
comP	Acinetobacter baylyi ADP1	38.621	100	0.392
pilA/pilAII	Pseudomonas stutzeri DSM 10701	37.838	100	0.392
pilA	Acinetobacter baumannii strain A118	38.095	100	0.392
pilA/pilAI	Pseudomonas stutzeri DSM 10701	36.806	100	0.371
pilA	Haemophilus influenzae Rd KW20	36.806	100	0.371

Detailed information NTG0595629