Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K3169_RS05235 Genome accession   NZ_CP081201
Coordinates   1206683..1208377 (-) Length   564 a.a.
NCBI ID   WP_231667863.1    Uniprot ID   -
Organism   Pseudomonas phytophila strain ICMP 23753     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1201683..1213377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3169_RS05210 (K3169_05170) - 1203263..1203730 (+) 468 WP_027902142.1 hypothetical protein -
  K3169_RS05215 (K3169_05175) yacG 1203768..1203968 (-) 201 WP_027902143.1 DNA gyrase inhibitor YacG -
  K3169_RS05220 (K3169_05180) coaE 1203965..1204588 (-) 624 WP_263270468.1 dephospho-CoA kinase -
  K3169_RS05225 (K3169_05185) pilD 1204585..1205457 (-) 873 WP_263270470.1 prepilin peptidase Machinery gene
  K3169_RS05230 (K3169_05190) pilC 1205460..1206680 (-) 1221 WP_231667864.1 type II secretion system F family protein Machinery gene
  K3169_RS05235 (K3169_05195) pilB 1206683..1208377 (-) 1695 WP_231667863.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3169_RS05240 (K3169_05200) pilA2 1208602..1209033 (+) 432 WP_231667862.1 pilin Machinery gene
  K3169_RS05245 (K3169_05205) nadC 1209449..1210297 (-) 849 WP_263270475.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K3169_RS05250 (K3169_05210) - 1210656..1212908 (+) 2253 WP_122708265.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62172.65 Da        Isoelectric Point: 6.3042

>NTDB_id=595628 K3169_RS05235 WP_231667863.1 1206683..1208377(-) (pilB) [Pseudomonas phytophila strain ICMP 23753]
MSDVVLTGLAKQLVAAELLTEKATQQAFLQARRDKVSLISYLVQNRLVKALALAEMSSDQFGLPFLDLNCLNKEEQPRGV
VSEKLIRQHHALPLWKRGNKLYIGISDPANQQAITDLQFNTGLAIEAILVEDDKLTDAIEKFFDGNASLGEMGDVDLSLE
VVSGGEVKETSLGQDDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKTFRVRLRTDGILHEVAKPPIHLASRIAARLKV
MASLDISERRKPQDGRVKLRISKTKAIDFRMNTLPTLWGEKIVMRLLDPTTAKMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPHCKKEADIPHDTLISEGFPADK
IGTFKIYTPVGCDQCNGGYKGRVGIYEVVKKTPALERIIMEEGNSLDISVQMRKDGFNDLRTSGLIKAMQGVTSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=595628 K3169_RS05235 WP_231667863.1 1206683..1208377(-) (pilB) [Pseudomonas phytophila strain ICMP 23753]
ATGAGTGACGTTGTCCTGACCGGTTTGGCCAAGCAATTGGTAGCTGCCGAACTTTTGACTGAAAAAGCGACGCAGCAGGC
GTTTCTGCAAGCCCGGCGCGACAAGGTTTCGCTGATCAGTTATCTGGTGCAAAACCGTCTGGTCAAAGCCCTAGCTCTGG
CGGAAATGTCGTCGGACCAATTCGGTCTGCCGTTTCTCGACCTTAATTGCCTTAATAAAGAAGAGCAGCCGAGAGGTGTG
GTGAGTGAAAAGCTCATCCGTCAGCACCATGCCCTGCCGCTGTGGAAGCGCGGCAATAAGCTTTATATCGGTATCTCCGA
CCCGGCCAACCAGCAAGCCATTACCGATCTGCAGTTCAACACCGGGCTCGCCATCGAAGCGATTCTGGTAGAAGACGACA
AGCTCACTGACGCCATCGAAAAGTTTTTCGATGGCAACGCTTCTCTTGGCGAAATGGGTGACGTCGATCTCAGCCTGGAG
GTGGTGTCAGGCGGGGAAGTCAAAGAGACGTCACTGGGTCAGGATGACGCCGACGATGCGCCGGTGGTGCGCTTCGTCAA
CAAGATGCTGATGGACGCCATCCGCCTCGGCTCGTCCGACCTGCACTTTGAGCCCTACGAAAAGACCTTTCGGGTTCGCT
TGCGCACGGACGGCATTCTGCATGAAGTCGCCAAGCCCCCTATCCACCTTGCAAGCCGCATTGCAGCGCGTTTGAAGGTC
ATGGCCAGCCTGGATATTTCCGAGCGCCGCAAACCGCAGGATGGTCGGGTCAAGCTGCGCATATCGAAAACCAAGGCGAT
TGATTTCCGGATGAACACCCTGCCGACGCTGTGGGGGGAAAAGATCGTGATGCGTCTGCTGGATCCGACCACGGCAAAAA
TGGGCATTGATGCATTGGGCTACGAGCCTGAGCAGAAAGCCCTGTACCTGGAAGCACTTAAACAACCGCAAGGCATGATT
CTGGTCACAGGCCCTACCGGCTCGGGTAAAACCGTGTCGCTGTACACCGGCCTGAATATTCTCAACACCGTGGATATCAA
TATCTCCACCGCCGAAGATCCAGTTGAAATCAACCTGGAAGGCGTCAACCAGGTCAACGTCAATCCCCGTCAAGGAATGG
ACTTCTCCCAGGCACTTCGGGCTTTTTTGCGTCAGGATCCTGACGTGATCATGGTCGGTGAGATTCGCGATCTGGAAACG
GCAGAAATCGCCATCAAGGCTTCGCAAACCGGGCACATGGTCTTGTCCACACTGCACACCAACAGCGCCGCCGAAACCCT
GACCCGCTTGCACCACATGGGTGTGGCCGCGTTCAACATTGCTACTGCCATCAACCTGATCATCGCTCAGCGTCTGGCGC
GCAAGCTGTGCCCGCACTGCAAGAAAGAAGCGGACATTCCTCACGACACCCTGATAAGCGAAGGCTTCCCGGCAGACAAG
ATCGGTACGTTCAAGATCTATACCCCGGTGGGCTGCGACCAGTGCAATGGCGGCTATAAAGGCCGCGTGGGGATTTACGA
AGTGGTCAAGAAGACCCCGGCGCTGGAGCGCATCATCATGGAAGAAGGCAACTCACTGGATATTTCTGTGCAGATGCGCA
AGGACGGCTTTAATGACCTGCGCACTTCCGGCCTGATCAAAGCCATGCAGGGCGTCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.584

99.645

0.564
  pilB Acinetobacter baylyi ADP1

56.05

99.645

0.559
  pilB Legionella pneumophila strain ERS1305867

53.71

100

0.539
  pilB Vibrio cholerae strain A1552

50.887

100

0.509
  pilB Vibrio campbellii strain DS40M4

47.957

99.823

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

49.088

97.163

0.477
  pilF Neisseria gonorrhoeae MS11

47.237

99.468

0.47
  pilF Thermus thermophilus HB27

37.611

99.468

0.374