Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K3819_RS27340 Genome accession   NZ_CP081200
Coordinates   6011867..6012901 (-) Length   344 a.a.
NCBI ID   WP_221398268.1    Uniprot ID   -
Organism   Pseudomonas sp. 3-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6006867..6017901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3819_RS27310 (K3819_27310) ruvX 6007075..6007512 (+) 438 WP_007953334.1 Holliday junction resolvase RuvX -
  K3819_RS27315 (K3819_27315) pyrR 6007594..6008097 (+) 504 WP_085646969.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  K3819_RS27320 (K3819_27320) - 6008125..6009129 (+) 1005 WP_007913665.1 aspartate carbamoyltransferase catalytic subunit -
  K3819_RS27325 (K3819_27325) - 6009126..6010397 (+) 1272 WP_085611974.1 dihydroorotase -
  K3819_RS27330 (K3819_27330) - 6010434..6010868 (-) 435 WP_064600078.1 TM2 domain-containing protein -
  K3819_RS27335 (K3819_27335) - 6011118..6011735 (+) 618 WP_039767935.1 C40 family peptidase -
  K3819_RS27340 (K3819_27340) pilT 6011867..6012901 (-) 1035 WP_221398268.1 type IV pilus twitching motility protein PilT Machinery gene
  K3819_RS27345 (K3819_27345) - 6013096..6013782 (+) 687 WP_221398269.1 YggS family pyridoxal phosphate-dependent enzyme -
  K3819_RS27350 (K3819_27350) proC 6013806..6014624 (+) 819 WP_064600085.1 pyrroline-5-carboxylate reductase -
  K3819_RS27355 (K3819_27355) - 6014635..6015225 (+) 591 WP_011336374.1 YggT family protein -
  K3819_RS27360 (K3819_27360) - 6015222..6015512 (+) 291 WP_221398270.1 DUF167 domain-containing protein -
  K3819_RS27365 (K3819_27365) - 6015578..6016717 (+) 1140 WP_039767930.1 homoserine O-acetyltransferase -
  K3819_RS27370 (K3819_27370) metW 6016725..6017345 (+) 621 WP_011336377.1 methionine biosynthesis protein MetW -
  K3819_RS27375 (K3819_27375) - 6017372..6017806 (+) 435 WP_221398271.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38099.79 Da        Isoelectric Point: 7.0157

>NTDB_id=595614 K3819_RS27340 WP_221398268.1 6011867..6012901(-) (pilT) [Pseudomonas sp. 3-2]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHQQVHELIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDKF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=595614 K3819_RS27340 WP_221398268.1 6011867..6012901(-) (pilT) [Pseudomonas sp. 3-2]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGTGCTTCCGACCTGCACCTGTCGGCCGGTCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGGCGGATCAATCTGCCGGCGCTGGATCACCAGCAAGTGCACGAGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGACTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCCGGCGTGGCGCGC
TTTCGGGTCAATGCCTTCAACCAGAACCGGGGCGCCGGGGCGGTGTTCCGGACCATCCCGTCAAAAGTGCTGACCATGGA
AGACCTGGCCATGGGCGACGTCTTTCGCAAGATCACCGATGCGCCGCGCGGGCTGGTGCTGGTGACCGGACCGACCGGCT
CCGGCAAGTCCACGACACTGGCGGCGATGATCGATTACCTCAACACCCATCGTCATCACCACATCCTGACCATCGAAGAT
CCGATCGAATTTGTCCACGAATCGCGCAAATGCCTGATCAATCAGCGCGAAGTCCATCGTGATACCCGCAGTTTCGCCAC
TGCGTTGCGCTCAGCGCTGCGCGAAGACCCGGATGTGATCCTGGTGGGCGAGATGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGGCACCTGGTGTTCGGTACCTTGCACACCACGTCGGCAGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGGGGACGAAAAGTCCATGGTGCGCTCGATGCTGTCGGAGTCGTTGCTGGCGGTAGTCTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGTCGGGTGGCGGCGCACGAGATCATGCTGGGTACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATACAGACCGGTGGCTCGCTGGGAATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAGGGCTTGATCAGCCGTGAACATGCGCGGGAGAAAGCGCGCACGCCAGATAAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.651

100

0.797
  pilT Acinetobacter baumannii strain A118

79.651

100

0.797
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.087

100

0.663
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

40.233

99.709

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401