Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M621_RS46060 Genome accession   NC_021659
Coordinates   4511712..4512716 (-) Length   334 a.a.
NCBI ID   WP_006327902.1    Uniprot ID   S4YKS9
Organism   Serratia plymuthica S13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4506712..4517716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M621_RS46030 (M621_21325) - 4507111..4507623 (+) 513 WP_004947987.1 SprT family zinc-dependent metalloprotease -
  M621_RS46035 (M621_21330) endA 4507725..4508420 (+) 696 WP_020439738.1 deoxyribonuclease I -
  M621_RS46040 (M621_21335) rsmE 4508605..4509336 (+) 732 WP_006327901.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M621_RS46045 (M621_21340) gshB 4509347..4510297 (+) 951 WP_004947997.1 glutathione synthase -
  M621_RS46050 (M621_21345) - 4510569..4511132 (+) 564 WP_004948000.1 YqgE/AlgH family protein -
  M621_RS46055 (M621_21350) ruvX 4511132..4511554 (+) 423 WP_004948002.1 Holliday junction resolvase RuvX -
  M621_RS46060 (M621_21355) pilT 4511712..4512716 (-) 1005 WP_006327902.1 type IV pilus twitching motility protein PilT Machinery gene
  M621_RS46065 (M621_21360) - 4512737..4513444 (+) 708 WP_004948006.1 YggS family pyridoxal phosphate-dependent enzyme -
  M621_RS46070 (M621_21365) proC 4513464..4514282 (+) 819 WP_006327903.1 pyrroline-5-carboxylate reductase -
  M621_RS46075 (M621_21370) - 4514314..4514868 (+) 555 WP_004948012.1 YggT family protein -
  M621_RS46080 (M621_21375) yggU 4514865..4515155 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  M621_RS46085 (M621_21380) - 4515173..4515766 (+) 594 WP_006327904.1 XTP/dITP diphosphatase -
  M621_RS46090 (M621_21385) hemW 4515759..4516898 (+) 1140 WP_006327905.1 radical SAM family heme chaperone HemW -
  M621_RS46095 (M621_21390) - 4516950..4517198 (-) 249 WP_020439741.1 DUF2442 domain-containing protein -
  M621_RS46100 (M621_21395) - 4517182..4517424 (-) 243 WP_006327907.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36206.62 Da        Isoelectric Point: 6.4998

>NTDB_id=59421 M621_RS46060 WP_006327902.1 4511712..4512716(-) (pilT) [Serratia plymuthica S13]
MDIDEFVALSVKHNASDLHLCAGHQPMLRIDGELQPLEGAEILTQERMHQLCGTLLQPPQRQLLQQHGQLDLALTLAGDI
RLRANFFLQSLGISIALRLIAGHCPTLAALAAPAIVPALLRREDGLILVTGATGSGKSTTLAAMIDDINQHQRRHVITLE
DPIEFMHHSRKSLIQQRELGRDSHSFDEALRAALREDPDVILLGELRDTTTIRLALTAAETGHLVLATLHTRSAPQAVDR
LVDVFPAEEKPYVRAQLAASLQAVIAQKLLSRPGGGRVAIFEVLTATAAVSSMIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGLLGDSV

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=59421 M621_RS46060 WP_006327902.1 4511712..4512716(-) (pilT) [Serratia plymuthica S13]
ATGGATATCGACGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCGGGCCATCAACCCAT
GTTGCGCATTGATGGTGAACTGCAACCGCTGGAAGGGGCGGAAATACTGACTCAGGAGCGAATGCACCAGCTGTGCGGCA
CGCTGCTGCAACCGCCGCAGCGACAGCTTTTGCAGCAGCATGGGCAGCTCGATCTGGCGTTGACGCTGGCGGGCGATATC
CGGCTGCGCGCCAATTTCTTTTTGCAAAGCCTGGGGATATCCATCGCGCTGCGGTTGATCGCCGGCCACTGCCCGACGCT
GGCGGCGTTAGCGGCACCGGCCATTGTCCCCGCGCTGCTGCGGCGTGAGGATGGTCTGATATTGGTCACCGGCGCGACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATTGACGACATCAATCAGCATCAACGCCGGCATGTCATCACGCTGGAA
GACCCGATCGAATTTATGCACCATAGCCGGAAGTCGTTGATTCAACAGCGCGAACTGGGCCGCGATAGCCACAGTTTTGA
CGAAGCGCTACGCGCCGCGCTGCGTGAAGATCCGGATGTGATCCTGTTGGGAGAACTGCGTGACACCACCACCATCCGTC
TGGCGCTCACCGCGGCGGAAACCGGCCATTTGGTGCTGGCGACGCTGCATACCCGCAGCGCGCCGCAGGCGGTGGATCGT
TTGGTAGACGTTTTCCCGGCAGAAGAAAAGCCTTATGTGCGCGCACAGCTGGCTGCCAGCCTGCAGGCGGTGATTGCGCA
AAAGCTGCTAAGCAGGCCTGGTGGCGGGCGGGTGGCAATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAGCATGA
TCCGCGAAGGCAAAACCCATCAGCTCGCCAGCGTGCTGCAAACCGGCGCGCAGTCCGGCATGCAGACTTTTGAACAGGGG
TTGCAGCAGCGGATCGACGCCGGTTTGCTGGGAGATAGCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S4YKS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

98.802

0.533
  pilT Vibrio cholerae strain A1552

53.939

98.802

0.533
  pilT Neisseria meningitidis 8013

49.552

100

0.497
  pilT Acinetobacter baylyi ADP1

49.547

99.102

0.491
  pilT Neisseria gonorrhoeae MS11

49.547

99.102

0.491
  pilT Acinetobacter baumannii D1279779

49.697

98.802

0.491
  pilT Acinetobacter baumannii strain A118

49.697

98.802

0.491
  pilT Acinetobacter nosocomialis M2

49.697

98.802

0.491
  pilT Pseudomonas aeruginosa PAK

48.358

100

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.182

98.802

0.476
  pilT Legionella pneumophila strain ERS1305867

47.321

100

0.476
  pilT Legionella pneumophila strain Lp02

47.321

100

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.464
  pilU Acinetobacter baylyi ADP1

39.009

96.707

0.377
  pilU Pseudomonas stutzeri DSM 10701

38.462

97.305

0.374
  pilU Vibrio cholerae strain A1552

37.346

97.006

0.362

Multiple sequence alignment