Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K1516_RS02470 Genome accession   NZ_CP080573
Coordinates   543225..544352 (-) Length   375 a.a.
NCBI ID   WP_032127971.1    Uniprot ID   A0AA43AM75
Organism   Stenotrophomonas maltophilia strain FZD2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 538225..549352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1516_RS02455 (K1516_02465) - 539082..540497 (-) 1416 WP_049461652.1 DEAD/DEAH box helicase -
  K1516_RS02460 (K1516_02470) - 541475..542464 (+) 990 WP_153856227.1 fumarylacetoacetate hydrolase family protein -
  K1516_RS02465 (K1516_02475) maiA 542479..543147 (+) 669 WP_248859111.1 maleylacetoacetate isomerase -
  K1516_RS02470 (K1516_02480) pilU 543225..544352 (-) 1128 WP_032127971.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1516_RS02475 (K1516_02485) - 544474..544839 (+) 366 WP_005415160.1 DUF4398 domain-containing protein -
  K1516_RS02480 (K1516_02490) - 544836..545720 (+) 885 WP_164162109.1 hypothetical protein -
  K1516_RS02485 (K1516_02495) - 546045..546281 (+) 237 WP_005407964.1 YdcH family protein -
  K1516_RS02490 (K1516_02500) - 546582..547952 (+) 1371 WP_110710963.1 pyridoxal-phosphate dependent enzyme -
  K1516_RS02495 (K1516_02505) - 548076..549260 (+) 1185 WP_153856230.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41453.78 Da        Isoelectric Point: 6.3741

>NTDB_id=594146 K1516_RS02470 WP_032127971.1 543225..544352(-) (pilU) [Stenotrophomonas maltophilia strain FZD2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEGSLEEGMESFDQCL
FRLAKAGIIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGQPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=594146 K1516_RS02470 WP_032127971.1 543225..544352(-) (pilU) [Stenotrophomonas maltophilia strain FZD2]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGCATCCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGTGGGCTGGTGCTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGACACCCATGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAGGGCCGTCGCCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGGCTCGCTGGAGGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCGGGCATCATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCACGACCCATACGCCGATTTCTCGGCCGGGCAGCCAAGCATCACTCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.733

0.491
  pilU Acinetobacter baylyi ADP1

49.435

94.4

0.467
  pilU Vibrio cholerae strain A1552

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.81

95.467

0.437
  pilT Pseudomonas aeruginosa PAK

42.899

90.133

0.387
  pilT Acinetobacter baylyi ADP1

41.765

90.667

0.379
  pilT Acinetobacter baumannii strain A118

41.716

90.133

0.376
  pilT Acinetobacter nosocomialis M2

41.716

90.133

0.376
  pilT Acinetobacter baumannii D1279779

41.716

90.133

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.42

90.133

0.373
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368
  pilT Vibrio cholerae strain A1552

40.841

88.8

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.8

0.363
  pilT Neisseria meningitidis 8013

39.193

92.533

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.533

0.363