Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SCE1572_RS28260 Genome accession   NC_021658
Coordinates   7802864..7804003 (+) Length   379 a.a.
NCBI ID   WP_020737370.1    Uniprot ID   S4XPM2
Organism   Sorangium cellulosum So0157-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7797864..7809003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCE1572_RS28240 (SCE1572_27255) - 7798170..7798466 (+) 297 WP_020737366.1 hypothetical protein -
  SCE1572_RS28245 (SCE1572_27260) - 7798481..7799830 (-) 1350 WP_020737367.1 serine/threonine-protein kinase -
  SCE1572_RS28250 (SCE1572_27265) - 7799827..7801053 (-) 1227 WP_020737368.1 FHA domain-containing protein -
  SCE1572_RS28255 (SCE1572_27270) pilB 7801159..7802859 (+) 1701 WP_020737369.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SCE1572_RS28260 (SCE1572_27275) pilT 7802864..7804003 (+) 1140 WP_020737370.1 type IV pilus twitching motility protein PilT Machinery gene
  SCE1572_RS28265 (SCE1572_27280) lexA 7804207..7804899 (+) 693 WP_020737371.1 transcriptional repressor LexA -
  SCE1572_RS28270 (SCE1572_27285) - 7805078..7805353 (+) 276 WP_236643994.1 DUF4398 domain-containing protein -
  SCE1572_RS28275 (SCE1572_27290) - 7805350..7806513 (+) 1164 WP_020737373.1 OmpA family protein -
  SCE1572_RS28280 (SCE1572_27295) - 7806647..7808392 (+) 1746 WP_020737374.1 EB domain-containing protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42012.71 Da        Isoelectric Point: 6.9232

>NTDB_id=59401 SCE1572_RS28260 WP_020737370.1 7802864..7804003(+) (pilT) [Sorangium cellulosum So0157-2]
MTTELVGQQEGLRYTLQQLLRAMVEKGASDMHITSGTPPLLRIDGTVVPLKLPPLMPGDTRQLCYSVLSEEQVAAFEQRN
ELDLSFGMKGLARFRANIYMQRGAVAAAFRQIPFRIMNFDELGLPPVVLDIAAKPRGLVLVTGPTGSGKTTTLASIIDKI
NAEQRLHILTIEDPIEYLHPHKLSLVNQREIGSDTASFKDALRYALRQDPDVVLVGEMRDLETVESALTIAETGHLVFAT
LHTNSAISTINRIIDVFPPHQQSQVRQQLSFTLVAVMTQLLLPRANGPGRVMAMEVMIPNAAIRNLIREDKLHQIYSQMQ
VGQAGSGMQTMAQSLVSLYQRRMITLEEMFAAAADPDEIRAMLEPRPAMSSRNNLPKQP

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=59401 SCE1572_RS28260 WP_020737370.1 7802864..7804003(+) (pilT) [Sorangium cellulosum So0157-2]
ATGACCACGGAGCTCGTCGGACAGCAGGAGGGCCTCAGGTACACGCTCCAGCAGCTCCTCCGCGCGATGGTGGAGAAGGG
CGCGAGCGACATGCACATCACGAGCGGCACGCCGCCGCTCCTGCGCATCGACGGCACGGTGGTGCCGCTCAAGCTCCCGC
CGCTCATGCCGGGCGACACGAGGCAGCTCTGCTACTCGGTGCTGAGCGAGGAGCAGGTGGCGGCGTTCGAGCAGCGGAAC
GAGCTCGATCTGTCGTTCGGCATGAAGGGGCTCGCCCGCTTCCGCGCGAACATCTACATGCAGCGCGGCGCCGTGGCGGC
CGCCTTCCGGCAGATCCCCTTCCGGATCATGAACTTCGACGAGCTGGGCCTGCCGCCGGTCGTGCTCGACATCGCCGCCA
AGCCGCGCGGCCTCGTGCTCGTGACGGGCCCGACCGGCTCGGGCAAGACGACCACGCTCGCGAGCATCATCGACAAGATC
AACGCCGAGCAGCGCCTCCACATCCTCACGATCGAGGATCCGATCGAGTACCTGCACCCGCACAAGCTCTCGCTCGTGAA
CCAGCGCGAGATCGGCTCCGACACCGCGTCGTTCAAGGACGCCCTGCGCTACGCCCTGCGCCAGGATCCGGACGTCGTGC
TCGTCGGCGAGATGCGCGACCTGGAGACCGTCGAGTCGGCGCTGACGATCGCCGAGACGGGCCACCTCGTGTTCGCCACG
CTCCACACGAACTCGGCGATCTCGACGATCAACCGCATCATCGACGTCTTCCCCCCGCACCAGCAGTCGCAGGTGCGGCA
GCAGCTGTCGTTCACGCTCGTCGCCGTGATGACCCAGCTGCTCCTGCCGCGCGCGAACGGCCCGGGGCGCGTCATGGCGA
TGGAGGTCATGATCCCGAACGCCGCGATCCGGAACCTCATCCGCGAGGACAAGCTCCACCAGATCTACTCGCAGATGCAG
GTGGGCCAGGCCGGCAGCGGGATGCAGACGATGGCGCAGTCGCTCGTCTCGCTCTACCAGCGCCGGATGATCACGCTGGA
GGAGATGTTCGCCGCCGCCGCCGACCCCGACGAGATCCGGGCGATGCTCGAGCCCCGCCCGGCCATGAGCTCGCGGAACA
ACCTCCCGAAGCAGCCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(23-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S4XPM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56.105

90.765

0.509
  pilT Legionella pneumophila strain Lp02

52.663

89.182

0.47
  pilT Legionella pneumophila strain ERS1305867

52.663

89.182

0.47
  pilT Acinetobacter nosocomialis M2

50.585

90.237

0.456
  pilT Acinetobacter baumannii D1279779

50.585

90.237

0.456
  pilT Acinetobacter baumannii strain A118

50.585

90.237

0.456
  pilT Acinetobacter baylyi ADP1

50.585

90.237

0.456
  pilT Pseudomonas stutzeri DSM 10701

49.855

91.029

0.454
  pilT Pseudomonas aeruginosa PAK

49.854

90.501

0.451
  pilT Neisseria meningitidis 8013

50.301

87.599

0.441
  pilT Vibrio cholerae strain A1552

49.701

88.127

0.438
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.701

88.127

0.438
  pilT Neisseria gonorrhoeae MS11

50

87.599

0.438
  pilU Vibrio cholerae strain A1552

40.171

92.612

0.372
  pilU Pseudomonas stutzeri DSM 10701

40.401

92.084

0.372

Multiple sequence alignment