Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0H63_RS13770 Genome accession   NZ_CP080414
Coordinates   3164426..3165463 (-) Length   345 a.a.
NCBI ID   WP_220065175.1    Uniprot ID   -
Organism   Shewanella zhangzhouensis strain FJAT-52072     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3159426..3170463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H63_RS13735 (K0H63_13735) - 3159978..3160538 (-) 561 WP_220065170.1 DUF4136 domain-containing protein -
  K0H63_RS13740 (K0H63_13740) - 3160729..3161058 (+) 330 WP_220065171.1 hypothetical protein -
  K0H63_RS13745 (K0H63_13745) yciH 3161172..3161501 (+) 330 WP_220065172.1 stress response translation initiation inhibitor YciH -
  K0H63_RS13750 (K0H63_13750) - 3161510..3162073 (+) 564 WP_220067906.1 YqgE/AlgH family protein -
  K0H63_RS13755 (K0H63_13755) ruvX 3162073..3162492 (+) 420 WP_220065173.1 Holliday junction resolvase RuvX -
  K0H63_RS13760 (K0H63_13760) - 3162560..3163102 (-) 543 WP_220067907.1 glutathione peroxidase -
  K0H63_RS13765 (K0H63_13765) pilU 3163304..3164416 (-) 1113 WP_220065174.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H63_RS13770 (K0H63_13770) pilT 3164426..3165463 (-) 1038 WP_220065175.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H63_RS13775 (K0H63_13775) - 3165499..3166206 (+) 708 WP_220065176.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H63_RS13780 (K0H63_13780) proC 3166298..3167113 (+) 816 WP_220065177.1 pyrroline-5-carboxylate reductase -
  K0H63_RS13785 (K0H63_13785) - 3167159..3167707 (+) 549 WP_220065178.1 YggT family protein -
  K0H63_RS13790 (K0H63_13790) yggU 3167707..3167994 (+) 288 WP_220065179.1 DUF167 family protein YggU -
  K0H63_RS13795 (K0H63_13795) - 3168077..3168511 (+) 435 WP_220065180.1 DUF4426 domain-containing protein -
  K0H63_RS13800 (K0H63_13800) rdgB 3168643..3169239 (+) 597 WP_220065181.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H63_RS13805 (K0H63_13805) hemW 3169249..3170388 (+) 1140 WP_220065182.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38263.93 Da        Isoelectric Point: 6.9095

>NTDB_id=592673 K0H63_RS13770 WP_220065175.1 3164426..3165463(-) (pilT) [Shewanella zhangzhouensis strain FJAT-52072]
MEITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHAGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMAKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592673 K0H63_RS13770 WP_220065175.1 3164426..3165463(-) (pilT) [Shewanella zhangzhouensis strain FJAT-52072]
ATGGAAATCACAGAGTTACTGGCGTTTAGTGTAAAGCACAAGGCCTCGGATCTACACCTCTCTGCCGGTGTTCCCCCCAT
GATCCGGGTGGACGGTGAAGTGCGTAAAATCAATCTGCCTGCACTCGATCATGCCGGCGTACACTCCTTGGTGTACGACA
TTATGAATGACAAGCAGCGCAAAGACTATGAAGAGCACCTGGAAATCGACTTTTCCTTTGAAGTGCCCAACCTTGCCCGT
TTCCGTGTAAACGCCTTTAACCAATCCCGCGGCGCTGCTGCGGTGTTTCGTACCATTCCCAGCGAAATTCTGTCGCTGGA
GCAACTGGGCGCCCCGGACATCTTCAAAAAAATCGCCAGTTTCCCCCGTGGTCTGGTGCTGGTGACCGGCCCAACCGGTT
CCGGTAAGAGTACCACCCTGGCAGGCATGATTGACTACGTGAACGAAAACCGTCACGACCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCACCAAAATAAGCAGTGTCTTATCAACCAGCGGGAAGTGCATCGTCATACCCACAGCTTTAACGC
GGCCCTTCGCAGCGCACTGCGTGAAGACCCCGACGTTATTCTGGTGGGTGAGATGCGTGACCTTGAAACCATACGTCTGG
CGATGACAGCGGCAGAAACCGGTCACCTGGTTTTCGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTGTG
GTCGACGTATTCCCGGCCGGTGAAAAAGACATGGTGCGTACCATGTTGTCAGAATCCTTGCAGGCGGTAATTTCCCAGAC
GCTGATCAAGAAGATTGGCGGCGGCCGGGTAGCAGCCCACGAAATTATGATGGGTACCCCTGCGATTCGTAACCTTATCC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCCATTCAAACCGGTATGGCCCATGGCATGCAAACTCTCGAGCAGTGTCTG
CAGAATCTGGTGAACCGTGGCCTCATCACCCGTGAAGACGCCATGGCCAAGAGTTCCAACAAACAGGCAAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.409

99.13

0.797
  pilT Acinetobacter baumannii D1279779

80.409

99.13

0.797
  pilT Acinetobacter baumannii strain A118

80.409

99.13

0.797
  pilT Acinetobacter baylyi ADP1

77.551

99.42

0.771
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

76.812

100

0.768
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.857

99.42

0.426
  pilU Vibrio cholerae strain A1552

41.399

99.42

0.412
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394