Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H650_RS10125 Genome accession   NC_021500
Coordinates   2151969..2152949 (-) Length   326 a.a.
NCBI ID   WP_020455167.1    Uniprot ID   -
Organism   Enterobacter sp. R4-368     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2146969..2157949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H650_RS10105 (H650_10980) - 2147822..2148385 (+) 564 WP_017457580.1 YqgE/AlgH family protein -
  H650_RS10110 (H650_10985) ruvX 2148385..2148801 (+) 417 WP_017457579.1 Holliday junction resolvase RuvX -
  H650_RS10115 (H650_10990) - 2148955..2149647 (-) 693 WP_044489482.1 hypothetical protein -
  H650_RS10120 (H650_10995) - 2149714..2151972 (+) 2259 WP_020455166.1 FAD-dependent oxidoreductase -
  H650_RS10125 (H650_11000) pilT 2151969..2152949 (-) 981 WP_020455167.1 type IV pilus twitching motility protein PilT Machinery gene
  H650_RS10130 (H650_11005) - 2152967..2153671 (+) 705 WP_020455168.1 YggS family pyridoxal phosphate-dependent enzyme -
  H650_RS10135 (H650_11010) - 2153690..2154256 (+) 567 WP_017457574.1 YggT family protein -
  H650_RS10140 (H650_11015) yggU 2154253..2154543 (+) 291 WP_020455169.1 DUF167 family protein YggU -
  H650_RS10145 (H650_11020) - 2154552..2155145 (+) 594 WP_020455170.1 XTP/dITP diphosphatase -
  H650_RS10150 (H650_11025) hemW 2155138..2156274 (+) 1137 WP_020455171.1 radical SAM family heme chaperone HemW -
  H650_RS10155 (H650_11030) - 2156463..2157182 (-) 720 WP_020455172.1 DUF2884 domain-containing protein -
  H650_RS10160 (H650_11035) - 2157238..2157564 (-) 327 WP_020455173.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35405.46 Da        Isoelectric Point: 6.4354

>NTDB_id=59226 H650_RS10125 WP_020455167.1 2151969..2152949(-) (pilT) [Enterobacter sp. R4-368]
MDIEEIVALSVKHNVSDLHLCSGSVPRWRQQGRLEPAPFEIADLAGLIDLHLNTAQQRLWRERGQVDFALTTAAGHRLRA
GAFTRHGGVSLALRLLPQSCPLLSELDAPAPLSELLGRENGLILVTGATGSGKSTTLAAMVDYLNQHNDAHILTLEDPIE
FVHTSNCCLIQQREVGLHCMSFADGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDT
FPAQEKDPVRSQLAGNLQAVLAQKLEADKRGGRVALFELLINSPAVASLIREGKTHQLPGVMQTGQQAGMQTFAQSHQQR
VLQGWL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=59226 H650_RS10125 WP_020455167.1 2151969..2152949(-) (pilT) [Enterobacter sp. R4-368]
ATGGATATAGAAGAAATAGTGGCGCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGCAGTGGCTCTGTGCCGCG
CTGGCGGCAGCAAGGCCGTCTGGAACCTGCGCCGTTTGAAATAGCGGATCTCGCCGGGCTGATTGATTTGCACCTCAATA
CGGCTCAGCAGCGTCTGTGGCGAGAGCGGGGGCAGGTCGATTTTGCCCTGACGACAGCCGCGGGGCACCGGCTGCGGGCA
GGAGCCTTTACGCGTCACGGTGGTGTTTCGCTGGCGTTACGCTTGCTGCCGCAAAGCTGCCCACTGCTCAGCGAGCTGGA
CGCCCCTGCGCCGTTGAGCGAGTTACTTGGGCGGGAAAACGGCCTGATTCTGGTGACGGGCGCGACCGGCAGCGGCAAAT
CCACCACTCTGGCAGCCATGGTGGATTATCTGAATCAGCATAACGATGCGCATATTCTGACGCTGGAAGATCCGATCGAG
TTTGTTCACACCAGCAACTGCTGCCTGATTCAGCAGCGGGAAGTGGGTCTACATTGCATGTCATTTGCCGACGGCCTGCG
CGCGGCGCTGCGTGAGGATCCGGATGTGATCCTGCTCGGTGAGTTGCGCGACAGCGAGACCATCCGTCTGGCCCTGACCG
CCGCAGAAACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCGGCACAAGCGGTAGAGAGGTTGGTCGATACC
TTTCCGGCGCAAGAAAAAGATCCTGTGCGCAGCCAACTGGCGGGAAACCTGCAGGCGGTGCTGGCGCAAAAACTTGAGGC
GGACAAGCGGGGAGGGCGAGTGGCATTATTCGAGTTGCTGATCAACTCTCCGGCGGTGGCGAGCTTGATCCGCGAAGGCA
AAACCCACCAGTTGCCCGGCGTAATGCAAACCGGGCAACAGGCGGGGATGCAAACCTTTGCACAAAGCCACCAACAGCGA
GTGTTGCAAGGCTGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.515

100

0.521
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.515

100

0.521
  pilT Neisseria meningitidis 8013

49.091

100

0.497
  pilT Neisseria gonorrhoeae MS11

48.788

100

0.494
  pilT Acinetobacter baylyi ADP1

47.416

100

0.479
  pilT Acinetobacter baumannii strain A118

47.112

100

0.475
  pilT Acinetobacter baumannii D1279779

47.112

100

0.475
  pilT Acinetobacter nosocomialis M2

47.112

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.112

100

0.475
  pilT Legionella pneumophila strain ERS1305867

48.113

97.546

0.469
  pilT Legionella pneumophila strain Lp02

48.113

97.546

0.469
  pilT Pseudomonas stutzeri DSM 10701

48.101

96.933

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.426

97.239

0.442
  pilU Vibrio cholerae strain A1552

41.231

99.693

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

38.629

98.466

0.38

Multiple sequence alignment