Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KYK13_RS30480 Genome accession   NZ_CP079946
Coordinates   7486664..7487776 (-) Length   370 a.a.
NCBI ID   WP_223636833.1    Uniprot ID   -
Organism   Corallococcus sp. EGB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7481664..7492776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYK13_RS30465 - 7482316..7483713 (-) 1398 WP_370645452.1 sigma-54-dependent transcriptional regulator -
  KYK13_RS30470 - 7483752..7485290 (-) 1539 WP_223646845.1 nitrogen regulation protein NR(II) -
  KYK13_RS30475 - 7485352..7486608 (-) 1257 WP_223636830.1 type II secretion system F family protein -
  KYK13_RS30480 pilT 7486664..7487776 (-) 1113 WP_223636833.1 type IV pilus twitching motility protein PilT Machinery gene
  KYK13_RS30485 pilB 7487825..7489525 (-) 1701 WP_223636836.1 type IV-A pilus assembly ATPase PilB -
  KYK13_RS30490 - 7489735..7490661 (-) 927 WP_223636837.1 bifunctional riboflavin kinase/FAD synthetase -
  KYK13_RS30495 - 7490743..7491363 (+) 621 WP_223636838.1 tRNA (guanosine(46)-N(7))-methyltransferase TrmB -
  KYK13_RS30500 - 7491538..7492581 (+) 1044 WP_223636839.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40491.70 Da        Isoelectric Point: 8.2875

>NTDB_id=590496 KYK13_RS30480 WP_223636833.1 7486664..7487776(-) (pilT) [Corallococcus sp. EGB]
MANLHQLLKAMVEKGSSDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLS
RFRANIFMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALTIAETGHICYATLHTNSAVQTINR
ILDVFPPYQQPQVRAQLSFVLEGVMSQALVAKQGAPGRVLALEVMVPNPAIRNLIREDKVHQIYSSMQVGQAKYGMQTFN
QALAALLARRLISQDEAFGRSSDPEELRNILATGGAPGGAQRPAGGAGAR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=590496 KYK13_RS30480 WP_223636833.1 7486664..7487776(-) (pilT) [Corallococcus sp. EGB]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCTCTTCCGACCTCCACATCACCACCGGCTCGCCGCC
GCAGCTGCGCGTGGACGGTGAGCTCGTCCCGCTCAAGACGGCGCCGCTCACGCCCGTGGAGACCAAGCAGCTCTGCTACT
CCATCCTCACGGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTGTCGTTCGGCGTGAAGGGCCTGTCG
CGCTTCCGCGCGAACATCTTCATGCAGCGCGGCGCGGTGGCCGGCGCGTTCCGGACCATTCCCTTCAAGATTTTGACCTT
CCAGGAGCTGGGCCTGCCGCCGGTGGTGGCGGAGCTGGTGAAGAAGCCGCGCGGCCTCATCCTGGTGACGGGCCCCACGG
GCTCCGGCAAGTCCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACCATCGAG
GACCCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTCGGTGCGGACACACGCAACTTCAA
GACGGCCCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTCGTCGGCGAGTTGCGCGACCTGGAGACCATTGAAG
CGGCGCTCACCATCGCGGAGACGGGCCACATCTGCTACGCGACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
ATCCTGGACGTCTTCCCGCCGTACCAGCAGCCCCAGGTGCGCGCGCAGCTGTCCTTCGTGTTGGAGGGCGTGATGAGCCA
GGCGCTCGTCGCCAAGCAGGGCGCCCCGGGCCGCGTGCTGGCCCTGGAGGTCATGGTGCCCAACCCCGCCATCCGGAACC
TCATCCGCGAGGACAAGGTCCACCAGATCTACTCCTCCATGCAGGTGGGCCAGGCGAAGTACGGCATGCAGACGTTCAAC
CAGGCGCTCGCCGCGCTCCTGGCGCGCCGGCTCATCTCCCAGGACGAGGCCTTCGGCCGTTCCTCCGACCCGGAGGAACT
GCGCAACATCCTCGCCACGGGCGGTGCGCCAGGGGGAGCCCAGCGGCCGGCCGGGGGAGCGGGCGCCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.275

98.378

0.495
  pilT Legionella pneumophila strain ERS1305867

54.03

90.541

0.489
  pilT Legionella pneumophila strain Lp02

54.03

90.541

0.489
  pilT Pseudomonas aeruginosa PAK

51.304

93.243

0.478
  pilT Pseudomonas stutzeri DSM 10701

51.304

93.243

0.478
  pilT Acinetobacter baylyi ADP1

51.014

93.243

0.476
  pilT Acinetobacter baumannii strain A118

50.725

93.243

0.473
  pilT Acinetobacter baumannii D1279779

50.725

93.243

0.473
  pilT Acinetobacter nosocomialis M2

50.435

93.243

0.47
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

90.27

0.441
  pilT Vibrio cholerae strain A1552

48.802

90.27

0.441
  pilT Neisseria gonorrhoeae MS11

44.868

92.162

0.414
  pilT Neisseria meningitidis 8013

44.868

92.162

0.414
  pilU Vibrio cholerae strain A1552

43.143

94.595

0.408
  pilU Pseudomonas stutzeri DSM 10701

41.011

96.216

0.395
  pilU Acinetobacter baylyi ADP1

40.346

93.784

0.378