Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JMJ87_RS02225 Genome accession   NZ_CP079839
Coordinates   458159..459139 (-) Length   326 a.a.
NCBI ID   WP_079974074.1    Uniprot ID   -
Organism   Salmonella enterica strain LHICA_E3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 453159..464139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMJ87_RS02195 (JMJ87_02195) endA 453617..454324 (+) 708 WP_079974072.1 deoxyribonuclease I -
  JMJ87_RS02200 (JMJ87_02200) rsmE 454400..455131 (+) 732 WP_001222491.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JMJ87_RS02205 (JMJ87_02205) gshB 455151..456098 (+) 948 WP_000593294.1 glutathione synthase -
  JMJ87_RS02210 (JMJ87_02210) - 456311..456874 (+) 564 WP_001053170.1 YqgE/AlgH family protein -
  JMJ87_RS02215 (JMJ87_02215) ruvX 456874..457290 (+) 417 WP_000099434.1 Holliday junction resolvase RuvX -
  JMJ87_RS02220 (JMJ87_02220) - 457338..458027 (-) 690 WP_000098331.1 IclR family transcriptional regulator -
  JMJ87_RS02225 (JMJ87_02225) pilT 458159..459139 (-) 981 WP_079974074.1 type IV pilus twitching motility protein PilT Machinery gene
  JMJ87_RS02230 (JMJ87_02230) - 459157..459861 (+) 705 WP_046093010.1 YggS family pyridoxal phosphate-dependent enzyme -
  JMJ87_RS02235 (JMJ87_02235) - 459880..460446 (+) 567 WP_001094848.1 YggT family protein -
  JMJ87_RS02240 (JMJ87_02240) yggU 460443..460733 (+) 291 WP_001277206.1 DUF167 family protein YggU -
  JMJ87_RS02245 (JMJ87_02245) - 460741..461334 (+) 594 WP_001174766.1 XTP/dITP diphosphatase -
  JMJ87_RS02250 (JMJ87_02250) hemW 461327..462463 (+) 1137 WP_023176917.1 radical SAM family heme chaperone HemW -
  JMJ87_RS02255 (JMJ87_02255) - 462555..463562 (-) 1008 WP_079814914.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36088.26 Da        Isoelectric Point: 7.2608

>NTDB_id=589862 JMJ87_RS02225 WP_079974074.1 458159..459139(-) (pilT) [Salmonella enterica strain LHICA_E3]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRRQGRMEIAPFIAPDVDRLLLDWLNDVQQYQWRTHGQLDFAVSLDGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQAPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYASKRCLIQQREIGQHCATFAAGLRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERVVDS
FPAQEKEPVRSQLAGNLRAVLSQKLEADRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQSR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=589862 JMJ87_RS02225 WP_079974074.1 458159..459139(-) (pilT) [Salmonella enterica strain LHICA_E3]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAGACAAGGACGGATGGAGATCGCGCCTTTTATCGCGCCTGATGTCGACAGGCTTCTACTTGATTGGCTTAATG
ACGTGCAACAATATCAGTGGCGAACGCATGGCCAGCTTGATTTCGCCGTTTCACTGGACGGTACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACATCGTTGGCGCTACGGCTCTTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GGCGCCACCGATAGTACCAGCACTACTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCAGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATTCTGACGCTGGAAGATCCTATTGAG
TACCGTTACGCCAGCAAGCGTTGCCTGATACAGCAACGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
TTCGGCATTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAAACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGAGTAGTGGATAGT
TTCCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGCAACCTGCGCGCCGTACTATCACAAAAACTGGAAGC
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGCGAAGGTA
AACTCCATCAGTTGGCCCATGTGATACAAACCGGGCAGCAGCAAGGGATGATGACATTCGCCCAGAGCGCGCAATCTCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(55-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.185

97.853

0.383
  pilU Pseudomonas stutzeri DSM 10701

36.446

100

0.371
  pilU Acinetobacter baylyi ADP1

36.923

99.693

0.368