Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K734_RS10125 Genome accession   NC_021286
Coordinates   2105964..2106998 (-) Length   344 a.a.
NCBI ID   WP_011235201.1    Uniprot ID   A0A4R7DHU5
Organism   Idiomarina loihiensis GSL 199     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2100964..2111998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K734_RS10100 (K734_09910) folB 2101174..2101557 (-) 384 WP_011235196.1 dihydroneopterin aldolase -
  K734_RS10105 (K734_09915) plsY 2101655..2102245 (+) 591 WP_011235197.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  K734_RS10110 (K734_09920) tsaD 2102229..2103263 (-) 1035 WP_011235198.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  K734_RS10115 (K734_09925) - 2103390..2104946 (+) 1557 WP_011235199.1 amidase -
  K734_RS10120 (K734_09930) - 2104924..2105964 (-) 1041 WP_011235200.1 phosphodiesterase -
  K734_RS10125 (K734_09935) pilT 2105964..2106998 (-) 1035 WP_011235201.1 type IV pilus twitching motility protein PilT Machinery gene
  K734_RS10130 (K734_09940) - 2107035..2107745 (+) 711 WP_016341381.1 YggS family pyridoxal phosphate-dependent enzyme -
  K734_RS10135 (K734_09945) proC 2107745..2108569 (+) 825 WP_011235203.1 pyrroline-5-carboxylate reductase -
  K734_RS10140 (K734_09950) - 2108578..2109105 (+) 528 WP_011235204.1 YggT family protein -
  K734_RS10145 (K734_09955) - 2109128..2109550 (+) 423 WP_011235205.1 DUF4426 domain-containing protein -
  K734_RS10150 (K734_09960) - 2109553..2111277 (-) 1725 WP_011235206.1 potassium/proton antiporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38257.76 Da        Isoelectric Point: 5.4778

>NTDB_id=58844 K734_RS10125 WP_011235201.1 2105964..2106998(-) (pilT) [Idiomarina loihiensis GSL 199]
MNVNELLAFSVKQNASDLHLSSGSVPMIRVDGDIRPLNQPEHQPDELKDMLFEVMTKGQWQEFEEKFECDFSFEIEGVAR
FRANVFTQRKGIAAAFRMIPAEVTTLEQLDAPEIFKDIVSANRGLVLVTGPTGSGKSTTLAAMVNYLNQNRALHILTIED
PIEFVHDNQKSLVSQREVHRDTRSFSAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVLGTLHTTSAPKTIDRI
IDVFPGDDKPMIRSMLSESLRAVISQSLLKRVGGGRVAAHEIMLSSPAIKNLIREDKVAQMYSSIQTGAERGMQTLDQAL
KKLLARGDIEAEEARRCAVTKEDF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=58844 K734_RS10125 WP_011235201.1 2105964..2106998(-) (pilT) [Idiomarina loihiensis GSL 199]
ATGAATGTTAACGAATTACTGGCCTTCAGTGTGAAACAAAATGCCTCGGACTTACATTTGTCCTCCGGCAGTGTCCCTAT
GATTAGAGTGGATGGGGACATTCGTCCGCTAAATCAACCCGAGCATCAGCCGGATGAGCTGAAAGACATGTTGTTTGAGG
TGATGACCAAAGGCCAGTGGCAGGAGTTCGAGGAAAAATTTGAGTGTGATTTTTCCTTTGAAATAGAAGGCGTTGCGCGT
TTCCGTGCCAACGTGTTTACCCAGCGCAAAGGCATTGCCGCCGCTTTTCGTATGATACCTGCCGAAGTCACCACTTTAGA
GCAACTGGATGCTCCGGAGATATTTAAGGACATTGTCAGTGCTAACCGGGGTTTAGTGTTAGTAACCGGACCGACTGGCT
CGGGTAAATCAACCACATTGGCGGCCATGGTTAATTATTTGAATCAAAACCGGGCGTTGCATATTTTAACCATTGAGGAC
CCCATAGAATTCGTTCACGACAATCAAAAAAGCCTGGTCAGCCAGCGTGAAGTTCATCGGGACACTCGCAGTTTCAGTGC
GGCCCTGCGCTCAGCACTGCGTGAAGATCCTGATGTGATTCTGGTGGGTGAAATGCGCGATTTAGAAACCATCCGTTTAG
CTATGACGGCAGCCGAAACCGGGCATTTAGTTTTAGGTACCTTGCATACAACTTCTGCACCTAAAACTATCGACCGCATT
ATTGACGTATTTCCGGGCGATGATAAACCGATGATCCGCTCCATGCTGTCAGAATCTTTACGTGCGGTTATCTCGCAGAG
CTTGCTAAAACGAGTTGGCGGTGGTCGTGTTGCCGCACATGAAATTATGCTGTCATCGCCAGCTATTAAAAACTTGATTC
GAGAAGACAAAGTGGCGCAAATGTACTCCTCTATTCAAACTGGAGCAGAGCGGGGCATGCAAACATTAGATCAGGCACTC
AAGAAATTACTGGCTCGTGGCGATATAGAGGCAGAAGAGGCACGCCGCTGTGCGGTGACTAAAGAGGATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R7DHU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

67.442

100

0.674
  pilT Vibrio cholerae strain A1552

68.955

97.384

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.955

97.384

0.672
  pilT Acinetobacter baylyi ADP1

66.57

100

0.666
  pilT Pseudomonas aeruginosa PAK

66.279

100

0.663
  pilT Acinetobacter baumannii D1279779

65.988

100

0.66
  pilT Acinetobacter baumannii strain A118

65.988

100

0.66
  pilT Acinetobacter nosocomialis M2

65.698

100

0.657
  pilT Legionella pneumophila strain Lp02

63.953

100

0.64
  pilT Legionella pneumophila strain ERS1305867

63.953

100

0.64
  pilT Neisseria meningitidis 8013

61.159

100

0.613
  pilT Neisseria gonorrhoeae MS11

60.87

100

0.61
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.488
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

38.824

98.837

0.384

Multiple sequence alignment