Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L336_RS03340 Genome accession   NC_021219
Coordinates   653958..655028 (+) Length   356 a.a.
NCBI ID   WP_015641811.1    Uniprot ID   R4PYP9
Organism   Candidatus Saccharimonas aalborgensis     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 648958..660028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L336_RS03315 (L336_0653) pilM 649480..650532 (+) 1053 WP_015641806.1 type IV pilus assembly protein PilM -
  L336_RS03320 (L336_0654) - 650529..651254 (+) 726 WP_015641807.1 hypothetical protein -
  L336_RS03325 (L336_0655) - 651254..651937 (+) 684 WP_015641808.1 hypothetical protein -
  L336_RS03330 (L336_0656) - 651937..652170 (+) 234 WP_015641809.1 hypothetical protein -
  L336_RS03335 (L336_0657) - 652182..653945 (+) 1764 WP_015641810.1 GspE/PulE family protein -
  L336_RS03340 (L336_0658) pilT 653958..655028 (+) 1071 WP_015641811.1 type IV pilus twitching motility protein PilT Machinery gene
  L336_RS03345 (L336_0659) - 655035..656240 (+) 1206 WP_015641812.1 type II secretion system F family protein -
  L336_RS03350 (L336_0660) - 656320..656826 (+) 507 WP_015641813.1 type II secretion system protein -
  L336_RS03355 (L336_0661) - 656900..657772 (+) 873 WP_015641814.1 A24 family peptidase -
  L336_RS03360 (L336_0662) - 657810..658529 (+) 720 WP_015641815.1 Tfp pilus assembly protein FimT/FimU -
  L336_RS03365 (L336_0663) - 658529..659122 (+) 594 WP_015641816.1 type II secretion system protein J -
  L336_RS03370 (L336_0664) - 659112..659822 (+) 711 WP_015641817.1 PilW family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39343.16 Da        Isoelectric Point: 5.8025

>NTDB_id=58759 L336_RS03340 WP_015641811.1 653958..655028(+) (pilT) [Candidatus Saccharimonas aalborgensis]
MNNPELRIEVLLEEVVRKRASDLHIQVGLPPMLRVDGALIPIVGFNPLDEQQVESLVFAILDQDQQQILLKDKEFDFSFA
FGTLGRFRVNAFHERGNLAAALRLIPNEIKSVTELGMPQVVLSFADYPRGLVLVTGPTGSGKSTTLAALVDKINSERAHH
IITIEDPIEFTHKSKKSVIVQREVHYDTYSFSAALRSSLRQDPDVVLIGEMRDLETISAAITIAETGHLVFATLHTNSAS
QSIDRMIDVFPPHQQPQIRAQLANILMAICSQRLVPAIGGGRVVAAEILIANPAVRNVIREGKSHQLDAIIQTGADQGMQ
TMDRTLVNLVQSGTVTYDNAREFAVDLTEFERLMRG

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=58759 L336_RS03340 WP_015641811.1 653958..655028(+) (pilT) [Candidatus Saccharimonas aalborgensis]
ATGAATAATCCAGAACTACGCATCGAAGTATTACTAGAAGAAGTTGTTCGCAAGCGCGCTTCAGACCTTCATATTCAGGT
GGGGTTACCACCAATGCTTCGTGTTGATGGTGCGTTGATACCAATTGTCGGCTTCAATCCTCTTGATGAGCAGCAAGTTG
AGTCACTTGTGTTTGCCATCTTGGACCAGGATCAGCAACAAATTCTTCTTAAAGACAAGGAGTTTGACTTCAGCTTTGCT
TTCGGGACGTTAGGGCGGTTTCGCGTTAACGCCTTTCACGAACGTGGTAACCTGGCTGCTGCGCTACGTCTCATCCCAAA
TGAGATCAAGTCTGTTACTGAACTGGGTATGCCGCAAGTAGTGCTCTCATTTGCTGACTATCCGCGCGGTCTTGTACTTG
TAACAGGACCAACTGGGTCTGGAAAATCAACCACGCTTGCTGCTCTTGTAGACAAAATCAACAGTGAACGCGCTCACCAC
ATTATCACAATTGAAGATCCAATTGAATTTACTCACAAGTCAAAGAAATCTGTTATTGTTCAGCGAGAAGTACATTACGA
TACCTACTCGTTCTCTGCAGCGCTGCGTAGCTCACTTCGTCAAGATCCTGATGTTGTGTTGATTGGTGAGATGCGCGATC
TCGAAACGATTTCTGCGGCAATTACGATTGCCGAAACCGGTCACTTAGTCTTTGCGACACTCCACACTAACTCGGCCTCG
CAGTCCATCGACCGTATGATCGATGTGTTTCCCCCTCACCAGCAACCACAGATTCGCGCACAGCTTGCTAATATTCTGAT
GGCAATCTGTAGTCAGCGCCTTGTACCTGCTATTGGTGGGGGAAGGGTAGTTGCAGCTGAAATTCTTATTGCAAATCCTG
CGGTGCGCAATGTTATCCGTGAAGGCAAGAGCCATCAGCTTGATGCCATTATCCAGACTGGTGCAGATCAGGGAATGCAG
ACCATGGATCGTACGCTTGTGAATCTGGTTCAGAGTGGAACGGTGACGTATGACAATGCTCGTGAGTTTGCCGTTGACCT
GACAGAGTTTGAGCGATTGATGAGAGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R4PYP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.755

97.472

0.534
  pilT Pseudomonas aeruginosa PAK

52.819

94.663

0.5
  pilT Legionella pneumophila strain Lp02

51.014

96.91

0.494
  pilT Legionella pneumophila strain ERS1305867

51.014

96.91

0.494
  pilT Acinetobacter baumannii D1279779

51.622

95.225

0.492
  pilT Acinetobacter nosocomialis M2

51.622

95.225

0.492
  pilT Acinetobacter baumannii strain A118

51.622

95.225

0.492
  pilT Acinetobacter baylyi ADP1

51.632

94.663

0.489
  pilT Pseudomonas stutzeri DSM 10701

51.327

95.225

0.489
  pilT Neisseria meningitidis 8013

49.706

95.506

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

92.697

0.475
  pilT Vibrio cholerae strain A1552

51.212

92.697

0.475
  pilT Neisseria gonorrhoeae MS11

49.412

95.506

0.472
  pilU Pseudomonas stutzeri DSM 10701

41.964

94.382

0.396
  pilU Vibrio cholerae strain A1552

41.212

92.697

0.382
  pilU Acinetobacter baylyi ADP1

38.988

94.382

0.368

Multiple sequence alignment