Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EGT68_RS04250 Genome accession   NZ_CP078042
Coordinates   874889..875926 (-) Length   345 a.a.
NCBI ID   WP_004913899.1    Uniprot ID   S7YGV1
Organism   Acinetobacter junii strain AJ_068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 855669..922331 874889..875926 within 0


Gene organization within MGE regions


Location: 855669..922331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT68_RS04150 (EGT68_004150) rhtC 856942..857559 (+) 618 WP_125272220.1 threonine export protein RhtC -
  EGT68_RS04155 (EGT68_004155) - 857597..857884 (-) 288 WP_004952694.1 PA4642 family protein -
  EGT68_RS04160 (EGT68_004160) minE 858067..858339 (-) 273 WP_004663449.1 cell division topological specificity factor MinE -
  EGT68_RS04165 (EGT68_004165) minD 858342..859154 (-) 813 WP_004913932.1 septum site-determining protein MinD -
  EGT68_RS04170 (EGT68_004170) minC 859220..859939 (-) 720 WP_125272219.1 septum site-determining protein MinC -
  EGT68_RS04175 (EGT68_004175) - 860057..861115 (-) 1059 WP_125272218.1 preprotein translocase subunit SecA -
  EGT68_RS04180 (EGT68_004180) - 861140..862057 (-) 918 WP_125272217.1 acyltransferase -
  EGT68_RS04185 (EGT68_004185) - 862447..863112 (+) 666 WP_042891288.1 OmpA family protein -
  EGT68_RS04190 (EGT68_004190) rep 863296..865335 (+) 2040 WP_004960037.1 DNA helicase Rep -
  EGT68_RS04195 (EGT68_004195) dut 865358..865810 (+) 453 WP_042891289.1 dUTP diphosphatase -
  EGT68_RS04200 (EGT68_004200) - 866003..867424 (+) 1422 WP_042891290.1 phosphomannomutase/phosphoglucomutase -
  EGT68_RS04205 (EGT68_004205) argB 867437..868336 (+) 900 WP_004913920.1 acetylglutamate kinase -
  EGT68_RS04210 (EGT68_004210) - 868540..869316 (+) 777 WP_112986357.1 GNAT family N-acetyltransferase -
  EGT68_RS04215 (EGT68_004215) - 869418..870266 (+) 849 WP_005403076.1 class II glutamine amidotransferase -
  EGT68_RS04220 (EGT68_004220) - 870549..871004 (+) 456 WP_004913914.1 bacteriohemerythrin -
  EGT68_RS04225 (EGT68_004225) - 871243..872322 (+) 1080 WP_125272216.1 hypothetical protein -
  EGT68_RS04230 (EGT68_004230) - 872322..872645 (+) 324 WP_004913910.1 RnfH family protein -
  EGT68_RS04235 (EGT68_004235) bamE 872701..873099 (-) 399 WP_004913908.1 outer membrane protein assembly factor BamE -
  EGT68_RS04240 (EGT68_004240) fur 873211..873648 (+) 438 WP_004913905.1 ferric iron uptake transcriptional regulator -
  EGT68_RS04245 (EGT68_004245) pilU 873745..874863 (-) 1119 WP_004913902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EGT68_RS04250 (EGT68_004250) pilT 874889..875926 (-) 1038 WP_004913899.1 type IV pilus twitching motility protein PilT Machinery gene
  EGT68_RS04255 (EGT68_004255) - 876054..876740 (+) 687 WP_004913896.1 YggS family pyridoxal phosphate-dependent enzyme -
  EGT68_RS04260 (EGT68_004260) - 876737..880333 (-) 3597 WP_125272215.1 AAA family ATPase -
  EGT68_RS04265 (EGT68_004265) - 880343..881605 (-) 1263 WP_171133353.1 exonuclease SbcCD subunit D -
  EGT68_RS04270 (EGT68_004270) - 881805..883271 (+) 1467 WP_042891296.1 DUF3375 domain-containing protein -
  EGT68_RS04275 (EGT68_004275) - 883284..884069 (+) 786 WP_042891297.1 DUF4194 domain-containing protein -
  EGT68_RS04280 (EGT68_004280) - 884069..887446 (+) 3378 WP_125272214.1 ATP-binding protein -
  EGT68_RS04285 (EGT68_004285) - 887439..888653 (+) 1215 WP_004799659.1 Wadjet anti-phage system protein JetD domain-containing protein -
  EGT68_RS04290 (EGT68_004290) - 888666..889448 (+) 783 WP_004960001.1 crotonase/enoyl-CoA hydratase family protein -
  EGT68_RS04295 (EGT68_004295) - 889515..889910 (-) 396 WP_004959998.1 SMI1/KNR4 family protein -
  EGT68_RS04300 (EGT68_004300) - 889914..890570 (-) 657 WP_125272213.1 hypothetical protein -
  EGT68_RS04305 (EGT68_004305) - 890621..890998 (-) 378 WP_042891303.1 VOC family protein -
  EGT68_RS04310 (EGT68_004310) - 891306..891752 (+) 447 WP_005403068.1 ABZJ_00895 family protein -
  EGT68_RS04315 (EGT68_004315) - 891829..892737 (+) 909 WP_125272212.1 TIGR01777 family oxidoreductase -
  EGT68_RS04320 (EGT68_004320) - 892723..893844 (-) 1122 WP_042891306.1 FAD-dependent oxidoreductase -
  EGT68_RS04325 (EGT68_004325) hemB 893917..894930 (-) 1014 WP_004913864.1 porphobilinogen synthase -
  EGT68_RS04330 (EGT68_004330) - 895188..895685 (+) 498 WP_004913863.1 thioesterase family protein -
  EGT68_RS04335 (EGT68_004335) - 895949..897085 (+) 1137 WP_042891308.1 HlyD family efflux transporter periplasmic adaptor subunit -
  EGT68_RS04340 (EGT68_004340) - 897092..898618 (+) 1527 WP_004913858.1 DHA2 family efflux MFS transporter permease subunit -
  EGT68_RS04345 (EGT68_004345) - 898791..898979 (+) 189 WP_004913856.1 heavy-metal-associated domain-containing protein -
  EGT68_RS04350 (EGT68_004350) cueR 899032..899490 (-) 459 WP_042891310.1 Cu(I)-responsive transcriptional regulator -
  EGT68_RS04355 (EGT68_004355) - 899501..901972 (-) 2472 WP_125272211.1 heavy metal translocating P-type ATPase -
  EGT68_RS04360 (EGT68_004360) - 902780..903136 (+) 357 WP_125272210.1 hypothetical protein -
  EGT68_RS04365 (EGT68_004365) - 903183..903500 (-) 318 WP_125272209.1 helix-turn-helix transcriptional regulator -
  EGT68_RS04370 (EGT68_004370) - 903800..904249 (+) 450 WP_125272208.1 hypothetical protein -
  EGT68_RS04375 (EGT68_004375) - 904301..904816 (+) 516 WP_125272207.1 hypothetical protein -
  EGT68_RS04380 (EGT68_004380) - 906553..907536 (+) 984 WP_125272206.1 hypothetical protein -
  EGT68_RS04385 (EGT68_004385) - 907642..912660 (+) 5019 WP_125272205.1 type ISP restriction/modification enzyme -
  EGT68_RS04390 (EGT68_004390) - 912731..914533 (+) 1803 WP_125272204.1 DUF262 domain-containing protein -
  EGT68_RS04395 (EGT68_004395) - 914635..915519 (+) 885 WP_125272203.1 hypothetical protein -
  EGT68_RS04400 (EGT68_004400) - 915649..915843 (+) 195 WP_125272202.1 hypothetical protein -
  EGT68_RS04405 (EGT68_004405) - 915844..917196 (+) 1353 WP_125272201.1 DUF3987 domain-containing protein -
  EGT68_RS04410 (EGT68_004410) - 917384..918724 (-) 1341 WP_125272200.1 tyrosine-type recombinase/integrase -
  EGT68_RS04420 (EGT68_004420) - 919742..920590 (+) 849 WP_235690344.1 ATP-binding cassette domain-containing protein -
  EGT68_RS04425 (EGT68_004425) - 920603..921097 (+) 495 WP_218999223.1 dual specificity protein phosphatase family protein -
  EGT68_RS16600 - 921122..921343 (+) 222 WP_228274933.1 hypothetical protein -
  EGT68_RS16605 - 921294..921530 (+) 237 WP_228274932.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38409.98 Da        Isoelectric Point: 6.4330

>NTDB_id=586466 EGT68_RS04250 WP_004913899.1 874889..875926(-) (pilT) [Acinetobacter junii strain AJ_068]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGTIFKDICDYPRGIVLVTGPTGSGKSTTLAAMIDYINEHRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KSLVSRGIISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=586466 EGT68_RS04250 WP_004913899.1 874889..875926(-) (pilT) [Acinetobacter junii strain AJ_068]
ATGGATATTACAGAATTACTCGCGTTTTCAGTAAAAAACGGTGCTTCGGACTTACACCTTTCAGCGGGTATGCCACCAAT
GATTCGCGTGGATGGTGAAGTTCGCCGTATCAATTTACCAGCATTAGAACATAAAGATGTACACCGTTTAGTGTACGACA
TTATGAATGACAAACAACGTCGTGATTACGAAGAAGATCTAGAAACGGACTTTTCATTTGAAGTACCAAACATTGCCCGT
TTCCGTGTAAACGCATTTAACCAAAACCGTGGTGCAGGTGCTGTATTCCGTACCATTCCATCTAAAGTCTTAACCATGGA
AGACTTGGGTTTAGGAACAATTTTCAAAGATATTTGTGACTACCCGCGTGGTATTGTTCTGGTCACTGGTCCAACGGGTT
CAGGTAAATCAACGACATTGGCTGCAATGATTGATTATATTAATGAGCATCGCTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACATCAATCAAAGAAATGTTTGATTAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAACGA
AGCACTTCGTTCAGCCCTTCGTGAAGACCCTGATATTATCCTAGTCGGTGAGATGCGTGACTTAGAGACAATTCGTCTTG
CTCTAACCGCAGCAGAAACAGGTCACTTAGTATTTGGTACTCTGCATACCACCTCAGCAGCAAAAACCATTGACCGTGTG
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGCGCGATGTTGTCCGAATCATTACAAGCAGTTATTTCACAAAC
ATTGTTGAAAAAGAATGGCGGTGGTCGTGTTGCAGCGCATGAGATCATGATCGGTATTCCTGCGATTCGTAACCTTGTTC
GTGAAAACAAAGTTGCACAGATGTACTCTGCGATTCAGACAGGTGCTAACTACGGCATGACCACACTCGATCAAAGTCTT
AAAAGCTTAGTTTCGAGAGGCATCATTAGCCCTCAAACAGCTCGTACTGTTGCTAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7YGV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.238

97.391

0.684
  pilT Vibrio cholerae strain A1552

70.238

97.391

0.684
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.791

97.101

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391