Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EGK58_RS11575 Genome accession   NZ_CP078027
Coordinates   2431509..2432630 (+) Length   373 a.a.
NCBI ID   WP_004783234.1    Uniprot ID   N9NI25
Organism   Acinetobacter variabilis strain AV_175     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2426509..2437630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGK58_RS11560 (EGK58_011560) - 2428492..2429598 (+) 1107 WP_125279468.1 acyltransferase family protein -
  EGK58_RS11565 (EGK58_011565) - 2429630..2430319 (-) 690 WP_125279469.1 YggS family pyridoxal phosphate-dependent enzyme -
  EGK58_RS11570 (EGK58_011570) pilT 2430443..2431480 (+) 1038 WP_004783232.1 type IV pilus twitching motility protein PilT Machinery gene
  EGK58_RS11575 (EGK58_011575) pilU 2431509..2432630 (+) 1122 WP_004783234.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EGK58_RS11580 (EGK58_011580) fur 2432697..2433134 (-) 438 WP_004783237.1 ferric iron uptake transcriptional regulator -
  EGK58_RS11585 (EGK58_011585) - 2433247..2433645 (+) 399 WP_004783238.1 outer membrane protein assembly factor BamE -
  EGK58_RS11590 (EGK58_011590) - 2433749..2434078 (-) 330 WP_005221123.1 RnfH family protein -
  EGK58_RS11595 (EGK58_011595) - 2434078..2435157 (-) 1080 WP_125279470.1 hypothetical protein -
  EGK58_RS11600 (EGK58_011600) - 2435317..2435772 (-) 456 WP_004783243.1 bacteriohemerythrin -
  EGK58_RS11605 (EGK58_011605) - 2436069..2436917 (-) 849 WP_034434176.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41688.94 Da        Isoelectric Point: 6.8892

>NTDB_id=586424 EGK58_RS11575 WP_004783234.1 2431509..2432630(+) (pilU) [Acinetobacter variabilis strain AV_175]
MDFNGLLNYMVEKKASDLFITADVEPSIKINGQIVPIGSVKLPGAAVGQLLNSIMTEKQRKEFADTRECNFAISNPDKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDELKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNMKAIVAQQLIPTIDGNGRRAAIEILINSPLIADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGEHLLRAHSNITFDGQT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=586424 EGK58_RS11575 WP_004783234.1 2431509..2432630(+) (pilU) [Acinetobacter variabilis strain AV_175]
ATGGACTTTAACGGCTTACTCAACTACATGGTAGAGAAAAAGGCCTCGGATCTGTTCATTACAGCCGATGTTGAACCCTC
TATCAAGATTAACGGTCAGATTGTACCGATTGGCTCGGTGAAATTGCCGGGCGCGGCTGTGGGGCAGCTACTTAACTCAA
TCATGACCGAGAAGCAGCGCAAGGAATTTGCTGACACACGTGAATGTAACTTCGCCATCAGCAACCCCGACAAATCAGCA
CGTTTCCGTGTTAGTGCTTTTCAGCAACGTGATCAGCCAGGCATGGTGCTGCGCCGTATTGAAACCGTCATTCCGACCAT
GGATGAGCTGAAGTTACCGCCTATTCTAAAAGAACTGGCGATGACTAAACGCGGAATCATTATTTTTGTAGGTGCAACCG
GTACGGGTAAATCGACTTCCCTCGCCTCTTTGATTGGCTACCGTAATGAAAATTCCAAAGGTCATATCATTACTATTGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGTTGTATCATTACCCAACGTGAAGTCGGTATCGATACTGACTCCTTTGA
AGTCGCATTGAAAAATACCCTGCGTCAAGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCTCGCGAAACCATGGACT
ATGCGATTGCCTTTGCAGAAACTGGTCACCTGGTTTTTGCGACCCTGCACGCCAACAACGCCAACCAGGCCATCGACCGT
ATCATTCACTTCTTCGAAGCCGACCGTCACAGCCAGCTGTTTATGGATCTGTCCCTGAACATGAAAGCCATTGTGGCACA
GCAACTAATTCCAACTATCGATGGTAATGGCCGCCGCGCAGCGATTGAAATCCTGATCAATTCGCCATTAATTGCGGATC
TGATCCGTAAAGGCGATGTGCATGAGATCAAAGATTTAATGAAACGTTCACGTGAATTGGGCATGCAAACCTTCGACCAG
GCATTATATGACTTGTATAAGGCCAAGCAGATTAGTTATAAAGATGCACTAAAACACGCCGACTCACCAAACGACCTGCG
TCTGCAAATCAAACTATCAGAAGAAGGTGGCGAGCATTTATTACGTGCGCATTCCAATATTACTTTTGATGGTCAAACCT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9NI25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.016

100

0.74
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.464

0.684
  pilU Vibrio cholerae strain A1552

57.835

94.102

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.775

92.761

0.397
  pilT Legionella pneumophila strain Lp02

43.155

90.08

0.389
  pilT Legionella pneumophila strain ERS1305867

43.155

90.08

0.389
  pilT Acinetobacter nosocomialis M2

41.983

91.957

0.386
  pilT Acinetobacter baumannii D1279779

41.983

91.957

0.386
  pilT Acinetobacter baumannii strain A118

41.983

91.957

0.386
  pilT Pseudomonas aeruginosa PAK

40.751

92.761

0.378
  pilT Acinetobacter baylyi ADP1

40.525

91.957

0.373
  pilT Vibrio cholerae strain A1552

39.601

94.102

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.601

94.102

0.373
  pilT Neisseria meningitidis 8013

40.173

92.761

0.373
  pilT Neisseria gonorrhoeae MS11

40.173

92.761

0.373
  pilT Pseudomonas stutzeri DSM 10701

39.595

92.761

0.367