Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLOPA_RS11415 Genome accession   NC_021182
Coordinates   2455113..2456168 (+) Length   351 a.a.
NCBI ID   WP_015615586.1    Uniprot ID   R4K2F1
Organism   Clostridium pasteurianum BC1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2450113..2461168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOPA_RS11395 (Clopa_2415) - 2450489..2451148 (+) 660 WP_015615582.1 O-methyltransferase -
  CLOPA_RS11400 (Clopa_2416) - 2451141..2452370 (+) 1230 WP_015615583.1 U32 family peptidase -
  CLOPA_RS11405 (Clopa_2417) - 2452614..2454107 (+) 1494 WP_242834298.1 penicillin-binding transpeptidase domain-containing protein -
  CLOPA_RS11410 (Clopa_2418) sigK 2454231..2454932 (+) 702 WP_015615585.1 RNA polymerase sporulation sigma factor SigK -
  CLOPA_RS11415 (Clopa_2419) pilT 2455113..2456168 (+) 1056 WP_015615586.1 type IV pilus twitching motility protein PilT Machinery gene
  CLOPA_RS11420 (Clopa_2420) - 2456398..2457672 (+) 1275 WP_015615587.1 glycosyltransferase family 2 protein -
  CLOPA_RS11425 (Clopa_2421) ftsA 2457974..2459233 (+) 1260 WP_015615588.1 cell division protein FtsA -
  CLOPA_RS11430 (Clopa_2422) ftsZ 2459249..2460355 (+) 1107 WP_015615589.1 cell division protein FtsZ -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39211.36 Da        Isoelectric Point: 7.5099

>NTDB_id=58552 CLOPA_RS11415 WP_015615586.1 2455113..2456168(+) (pilT) [Clostridium pasteurianum BC1]
MKKLNELLKLTLDNKASDLHITVGVPPVIRVNGELNAIDEDKLLPTDTEKYVRELLQSKFNEYCEKGEIDLSYSVAGIGR
FRVNAYKQRGSDAIAIRNVGLKIPELKELNFPKVINDVIQAQRGLILVTGPTGSGKSTTLAAMINEINFTRKSHIITLED
PIEYMHKHNKSIINQREIGRDSYSYASALKAILREDPDVILIGEMRDLETISIAITAAETGHLVFSTLHTIGAAKTIDRI
TDVFPPFQQQQIKIQLAAVLKGIISQQLIPNEDNTGRVAALEIMINTPAIQNLIREGKTHQLQSLIQTGNKYGMKTMDMS
LTELYKRGIISQESALTYSVDRELISKLLMF

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=58552 CLOPA_RS11415 WP_015615586.1 2455113..2456168(+) (pilT) [Clostridium pasteurianum BC1]
TTGAAAAAACTGAATGAACTATTGAAATTAACATTAGATAATAAAGCATCAGATTTACATATAACTGTTGGAGTACCACC
GGTTATAAGAGTAAATGGTGAACTAAATGCCATAGATGAAGACAAGCTTCTTCCTACTGATACAGAAAAGTATGTGAGAG
AATTACTTCAATCTAAGTTTAATGAATACTGTGAAAAAGGAGAAATAGATTTGTCCTATTCTGTAGCTGGTATAGGGAGA
TTTAGAGTTAATGCGTATAAGCAGAGAGGTAGTGATGCTATTGCCATTAGAAATGTAGGTTTAAAAATACCAGAACTTAA
AGAATTGAATTTCCCAAAAGTTATAAATGATGTGATTCAAGCACAAAGAGGTTTGATTTTAGTTACAGGGCCAACGGGAA
GTGGCAAAAGTACTACTCTTGCTGCCATGATAAATGAAATTAATTTTACAAGAAAGTCACATATAATAACGCTAGAAGAT
CCAATAGAATACATGCATAAACATAATAAGTCAATTATAAATCAAAGAGAAATAGGTAGGGATAGCTATAGCTATGCATC
TGCTTTAAAGGCTATTTTAAGAGAGGATCCTGATGTTATTTTGATTGGAGAGATGAGAGACTTAGAGACAATATCTATAG
CTATTACAGCGGCTGAAACAGGTCATCTTGTGTTTTCTACTTTGCACACCATAGGTGCAGCTAAAACCATAGACAGAATA
ACAGATGTATTTCCACCTTTTCAGCAGCAGCAAATAAAAATTCAGCTTGCCGCAGTACTTAAGGGTATAATATCTCAACA
GTTAATACCTAATGAAGACAATACAGGTAGAGTAGCAGCACTTGAAATAATGATTAATACTCCTGCAATACAAAATCTTA
TAAGAGAAGGAAAAACTCATCAATTACAATCACTTATACAAACTGGTAATAAATACGGTATGAAAACAATGGATATGTCT
TTGACTGAATTATATAAAAGAGGTATTATATCACAGGAATCTGCTTTAACTTATAGTGTGGACAGAGAACTTATTTCAAA
ATTATTGATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R4K2F1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.592

96.296

0.487
  pilT Acinetobacter baylyi ADP1

50.898

95.157

0.484
  pilT Acinetobacter baumannii strain A118

50.595

95.726

0.484
  pilT Acinetobacter baumannii D1279779

50.595

95.726

0.484
  pilT Acinetobacter nosocomialis M2

50.595

95.726

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48

99.715

0.479
  pilT Pseudomonas stutzeri DSM 10701

49.408

96.296

0.476
  pilT Neisseria gonorrhoeae MS11

47.564

99.43

0.473
  pilT Neisseria meningitidis 8013

47.564

99.43

0.473
  pilT Legionella pneumophila strain Lp02

48.094

97.151

0.467
  pilT Legionella pneumophila strain ERS1305867

48.094

97.151

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.091

94.017

0.462
  pilT Vibrio cholerae strain A1552

49.091

94.017

0.462
  pilU Vibrio cholerae strain A1552

42.353

96.866

0.41
  pilU Pseudomonas stutzeri DSM 10701

42.012

96.296

0.405
  pilU Acinetobacter baylyi ADP1

41.716

96.296

0.402

Multiple sequence alignment