Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RORB6_RS22720 Genome accession   NC_021066
Coordinates   4948518..4949498 (+) Length   326 a.a.
NCBI ID   WP_015585749.1    Uniprot ID   -
Organism   Raoultella ornithinolytica B6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4943518..4954498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RORB6_RS22690 (RORB6_22540) - 4943829..4944875 (-) 1047 WP_004867511.1 sugar ABC transporter substrate-binding protein -
  RORB6_RS22695 (RORB6_22545) hemW 4945188..4946327 (-) 1140 WP_015585745.1 radical SAM family heme chaperone HemW -
  RORB6_RS22700 (RORB6_22550) - 4946320..4946913 (-) 594 WP_015585746.1 XTP/dITP diphosphatase -
  RORB6_RS22705 (RORB6_22555) yggU 4946926..4947216 (-) 291 WP_004867501.1 DUF167 family protein YggU -
  RORB6_RS22710 (RORB6_22560) - 4947213..4947779 (-) 567 WP_015585747.1 YggT family protein -
  RORB6_RS22715 (RORB6_22565) - 4947797..4948501 (-) 705 WP_015585748.1 YggS family pyridoxal phosphate-dependent enzyme -
  RORB6_RS22720 (RORB6_22570) pilT 4948518..4949498 (+) 981 WP_015585749.1 type IV pilus twitching motility protein PilT Machinery gene
  RORB6_RS22725 (RORB6_22575) - 4949834..4950487 (+) 654 WP_015585750.1 LuxR C-terminal-related transcriptional regulator -
  RORB6_RS22730 (RORB6_22580) ruvX 4950557..4950973 (-) 417 WP_004867490.1 Holliday junction resolvase RuvX -
  RORB6_RS22735 (RORB6_22585) - 4950973..4951536 (-) 564 WP_015585751.1 YqgE/AlgH family protein -
  RORB6_RS22745 (RORB6_22595) gshB 4952157..4953104 (-) 948 WP_004867487.1 glutathione synthase -
  RORB6_RS22750 (RORB6_22600) rsmE 4953125..4953856 (-) 732 WP_015585753.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35772.14 Da        Isoelectric Point: 7.2940

>NTDB_id=58274 RORB6_RS22720 WP_015585749.1 4948518..4949498(+) (pilT) [Raoultella ornithinolytica B6]
MELEEIVALSVKHNVSDLHLCNTSAARWRRQGKLEPAPFHTPDIVKWLDRWLDEKQRAHWRANGQVDFALAPVGVPRLRA
SAFSHARGISLALRLLPESCPRIEALRVPAALTELLNEESGLILVTGATGSGKSTTLAAMVNHLNQQREGHILTLEDPVE
FVHHSERCLIQQREIGRDCPSFSSALKGALRQDPDVILLGELRDNETIRLALTAAETGHLVMATLHTRGAAQAIERLVDV
FPAQEKEQIRSQLAGSLCAVLAQKLLPTAPEGRVALYELLVNTPAVANLIREGKTHQLPGIMQTGQQAGMLTFTQSFQQR
VAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=58274 RORB6_RS22720 WP_015585749.1 4948518..4949498(+) (pilT) [Raoultella ornithinolytica B6]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATACCTCGGCAGCGCG
CTGGCGTCGGCAGGGAAAGCTTGAGCCGGCGCCATTTCACACGCCGGATATTGTGAAATGGCTTGACAGGTGGCTCGATG
AGAAACAGCGCGCGCACTGGCGGGCCAACGGACAGGTGGATTTTGCTTTAGCCCCCGTCGGCGTCCCGCGCCTTCGCGCC
AGCGCATTTTCTCACGCCCGGGGTATTTCATTAGCGCTACGTCTGTTGCCGGAAAGCTGCCCCCGGATTGAGGCGCTGCG
TGTACCGGCGGCGCTGACGGAACTGCTGAATGAAGAAAGCGGTTTGATTCTGGTGACCGGCGCCACCGGCAGCGGTAAGT
CGACCACCCTCGCGGCGATGGTCAACCATCTCAACCAGCAGCGTGAAGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCCATCACAGCGAGCGATGCCTGATTCAACAACGTGAAATCGGACGTGACTGCCCCTCTTTTTCCTCGGCGCTGAA
GGGGGCGCTGCGCCAGGATCCCGATGTGATTCTGTTGGGTGAGCTGCGCGACAACGAAACGATCCGCCTGGCGCTGACCG
CCGCGGAGACCGGACATCTGGTGATGGCCACGCTGCATACCCGAGGTGCGGCACAGGCGATTGAGCGTCTGGTGGATGTC
TTCCCCGCGCAGGAAAAAGAACAAATACGCAGCCAGCTAGCGGGAAGTTTATGTGCGGTGCTGGCACAAAAATTATTGCC
GACAGCGCCAGAGGGAAGAGTTGCGCTATATGAGCTTTTGGTGAATACCCCAGCGGTAGCGAATTTGATCCGCGAGGGGA
AAACTCATCAATTGCCGGGGATTATGCAAACCGGTCAACAAGCCGGAATGCTGACTTTTACGCAAAGTTTTCAACAACGT
GTGGCAGCGGGCCTGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

46.364

100

0.469
  pilT Neisseria meningitidis 8013

46.364

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Acinetobacter baumannii D1279779

46.061

100

0.466
  pilT Acinetobacter baumannii strain A118

46.061

100

0.466
  pilT Acinetobacter nosocomialis M2

45.758

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter baylyi ADP1

45.152

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.289

100

0.457
  pilT Legionella pneumophila strain Lp02

45.289

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

40.483

100

0.411
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Acinetobacter baylyi ADP1

38.82

98.773

0.383

Multiple sequence alignment