Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAX72_RS20500 Genome accession   NZ_CP077696
Coordinates   4156308..4157288 (-) Length   326 a.a.
NCBI ID   WP_001055647.1    Uniprot ID   A0A3V9PQV4
Organism   Salmonella enterica subsp. enterica strain CFSAN058598     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4151308..4162288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAX72_RS20470 (DAX72_020470) endA 4151770..4152477 (+) 708 WP_023972565.1 deoxyribonuclease I -
  DAX72_RS20475 (DAX72_020475) rsmE 4152554..4153285 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DAX72_RS20480 (DAX72_020480) gshB 4153305..4154252 (+) 948 WP_000593248.1 glutathione synthase -
  DAX72_RS20485 (DAX72_020485) - 4154468..4155031 (+) 564 WP_001053171.1 YqgE/AlgH family protein -
  DAX72_RS20490 (DAX72_020490) ruvX 4155031..4155447 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  DAX72_RS20495 (DAX72_020495) - 4155494..4156180 (-) 687 WP_000098324.1 IclR family transcriptional regulator -
  DAX72_RS20500 (DAX72_020500) pilT 4156308..4157288 (-) 981 WP_001055647.1 type IV pilus twitching motility protein PilT Machinery gene
  DAX72_RS20505 (DAX72_020505) - 4157306..4158010 (+) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  DAX72_RS20510 (DAX72_020510) - 4158029..4158595 (+) 567 WP_001094848.1 YggT family protein -
  DAX72_RS20515 (DAX72_020515) yggU 4158592..4158882 (+) 291 WP_001277205.1 DUF167 family protein YggU -
  DAX72_RS20520 (DAX72_020520) - 4158890..4159483 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  DAX72_RS20525 (DAX72_020525) hemW 4159476..4160612 (+) 1137 WP_001096527.1 radical SAM family heme chaperone HemW -
  DAX72_RS20530 (DAX72_020530) - 4160703..4161710 (-) 1008 WP_000252190.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36151.32 Da        Isoelectric Point: 7.5116

>NTDB_id=582359 DAX72_RS20500 WP_001055647.1 4156308..4157288(-) (pilT) [Salmonella enterica subsp. enterica strain CFSAN058598]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=582359 DAX72_RS20500 WP_001055647.1 4156308..4157288(-) (pilT) [Salmonella enterica subsp. enterica strain CFSAN058598]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V9PQV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.371
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362