Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KQ937_RS02290 Genome accession   NZ_CP077686
Coordinates   451026..452060 (+) Length   344 a.a.
NCBI ID   WP_284669275.1    Uniprot ID   -
Organism   Photobacterium damselae strain Pdd0906     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446026..457060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KQ937_RS02255 (KQ937_02255) - 446269..446673 (+) 405 WP_284669272.1 DUF2884 family protein -
  KQ937_RS02260 (KQ937_02260) hemW 446748..447908 (-) 1161 WP_284669273.1 radical SAM family heme chaperone HemW -
  KQ937_RS02265 (KQ937_02265) - 447908..448501 (-) 594 WP_044174415.1 XTP/dITP diphosphatase -
  KQ937_RS02270 (KQ937_02270) yggU 448603..448902 (-) 300 WP_036763214.1 DUF167 family protein YggU -
  KQ937_RS02275 (KQ937_02275) - 448899..449456 (-) 558 WP_005297682.1 YggT family protein -
  KQ937_RS02280 (KQ937_02280) proC 449468..450286 (-) 819 WP_005297684.1 pyrroline-5-carboxylate reductase -
  KQ937_RS02285 (KQ937_02285) - 450302..451003 (-) 702 WP_284669274.1 YggS family pyridoxal phosphate-dependent enzyme -
  KQ937_RS02290 (KQ937_02290) pilT 451026..452060 (+) 1035 WP_284669275.1 type IV pilus twitching motility protein PilT Machinery gene
  KQ937_RS02295 (KQ937_02295) pilU 452072..453178 (+) 1107 WP_005297691.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KQ937_RS02300 (KQ937_02300) ruvX 453506..453931 (-) 426 WP_005297693.1 Holliday junction resolvase RuvX -
  KQ937_RS02305 (KQ937_02305) - 453928..454491 (-) 564 WP_106142332.1 YqgE/AlgH family protein -
  KQ937_RS02310 (KQ937_02310) gshB 454645..455592 (-) 948 WP_005297697.1 glutathione synthase -
  KQ937_RS02315 (KQ937_02315) rsmE 455613..456344 (-) 732 WP_005297699.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38210.76 Da        Isoelectric Point: 6.1433

>NTDB_id=582270 KQ937_RS02290 WP_284669275.1 451026..452060(+) (pilT) [Photobacterium damselae strain Pdd0906]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALEHSEVHRLIFDIMNDAQRRQFEEELEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPMTIPTLESLNVPDVFYKIAQCQRGLVLVTGATGSGKSTTIAALVDYINEHTNRHILTIED
PIEFVHHSKRCLINQREVHRDTHSFQAALRSALREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKSIDRI
IDVFPGSDKSMVRSMLSESLRAVVSQSLLKCISGGRVASHEIMMATPAIRNLIREDKIAQMYSMIQTGSTMGMQTMEQSV
KMLVAQGLVEAEEGRRIVDSAQQI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=582270 KQ937_RS02290 WP_284669275.1 451026..452060(+) (pilT) [Photobacterium damselae strain Pdd0906]
ATGGATATCACCGAATTATTAGACTTCAGTGTAAAACATAACGCATCGGATCTGCATCTTTCAGCAGGTGTTCCACCTAT
GATCCGTGTAGATGGTGATGTACGCAAGTTAAGTTTACCTGCATTAGAGCACTCAGAGGTTCATCGTCTGATTTTTGATA
TCATGAATGATGCCCAGCGTCGTCAATTTGAAGAAGAGTTAGAAGTCGACTTCTCATTTGAATTACCCGATGTTGGCCGT
TTTCGTGTTAACGCCTTTCACCAATCTCGAGGTTGTTCTGCGGTCTTTCGTACTATCCCTATGACCATTCCAACCTTGGA
ATCTCTGAATGTGCCCGATGTTTTCTACAAAATAGCACAATGTCAGCGTGGATTGGTATTAGTTACAGGGGCTACAGGTT
CTGGTAAATCCACCACCATTGCGGCATTGGTTGATTATATTAATGAGCACACCAATCGTCATATCCTCACGATTGAAGAT
CCAATTGAATTTGTGCATCACAGTAAACGTTGTTTGATTAACCAACGAGAAGTGCATCGTGATACCCACAGTTTTCAAGC
CGCTTTGCGCTCGGCATTACGTGAAGATCCCGATGTGATTGTTGTGGGGGAATTGCGAGATCAAGAGACCATTAGTTTAG
CGTTAACTGCTGCAGAAACCGGACACTTGGTTTTAGGTACGCTACACACAAGTTCAGCAGCCAAAAGTATTGACCGTATT
ATCGATGTATTTCCTGGTAGTGATAAATCCATGGTTCGCTCCATGTTATCTGAGTCATTACGTGCAGTGGTTTCCCAAAG
TTTGTTGAAATGCATTAGCGGTGGTCGTGTGGCTTCCCACGAGATCATGATGGCAACCCCTGCGATTCGAAATTTGATCC
GCGAAGATAAGATTGCTCAGATGTATTCCATGATCCAAACCGGATCGACAATGGGAATGCAGACAATGGAACAGAGTGTG
AAGATGCTGGTTGCCCAAGGACTAGTGGAAGCAGAAGAAGGTCGTCGTATTGTCGACAGTGCCCAACAGATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

79.58

96.802

0.77
  pilT Vibrio cholerae strain A1552

79.58

96.802

0.77
  pilT Acinetobacter baumannii D1279779

70.448

97.384

0.686
  pilT Acinetobacter baumannii strain A118

70.448

97.384

0.686
  pilT Acinetobacter nosocomialis M2

70.149

97.384

0.683
  pilT Pseudomonas aeruginosa PAK

70.149

97.384

0.683
  pilT Acinetobacter baylyi ADP1

68.713

99.419

0.683
  pilT Pseudomonas stutzeri DSM 10701

69.851

97.384

0.68
  pilT Legionella pneumophila strain Lp02

69.301

95.64

0.663
  pilT Legionella pneumophila strain ERS1305867

69.301

95.64

0.663
  pilT Neisseria meningitidis 8013

65.282

97.965

0.64
  pilT Neisseria gonorrhoeae MS11

64.985

97.965

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

100

0.5
  pilU Vibrio cholerae strain A1552

41.159

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.13

93.605

0.366