Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KSL88_RS04020 Genome accession   NZ_CP077421
Coordinates   914197..915213 (+) Length   338 a.a.
NCBI ID   WP_224073185.1    Uniprot ID   -
Organism   Pectobacterium polaris strain QK413-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 909197..920213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSL88_RS03985 (KSL88_03980) - 909440..909703 (-) 264 WP_224073183.1 type II toxin-antitoxin system ParD family antitoxin -
  KSL88_RS03990 (KSL88_03985) hemW 909929..911071 (-) 1143 WP_224073184.1 radical SAM family heme chaperone HemW -
  KSL88_RS03995 (KSL88_03990) - 911064..911657 (-) 594 WP_039470191.1 XTP/dITP diphosphatase -
  KSL88_RS04000 (KSL88_03995) yggU 911675..911965 (-) 291 WP_010279110.1 DUF167 family protein YggU -
  KSL88_RS04005 (KSL88_04000) - 911962..912516 (-) 555 WP_010279107.1 YggT family protein -
  KSL88_RS04010 (KSL88_04005) proC 912566..913387 (-) 822 WP_224073807.1 pyrroline-5-carboxylate reductase -
  KSL88_RS04015 (KSL88_04010) - 913463..914176 (-) 714 WP_205555667.1 YggS family pyridoxal phosphate-dependent enzyme -
  KSL88_RS04020 (KSL88_04015) pilT 914197..915213 (+) 1017 WP_224073185.1 type IV pilus twitching motility protein PilT Machinery gene
  KSL88_RS04025 (KSL88_04020) - 915182..917014 (-) 1833 WP_224073186.1 diguanylate cyclase -
  KSL88_RS04030 (KSL88_04025) - 917667..919238 (-) 1572 WP_095992814.1 methyl-accepting chemotaxis protein -
  KSL88_RS04035 (KSL88_04030) nsrR 919565..919990 (+) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36689.95 Da        Isoelectric Point: 6.6534

>NTDB_id=581437 KSL88_RS04020 WP_224073185.1 914197..915213(+) (pilT) [Pectobacterium polaris strain QK413-1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSAQPSLSALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAVQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=581437 KSL88_RS04020 WP_224073185.1 914197..915213(+) (pilT) [Pectobacterium polaris strain QK413-1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTCGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGCGCGTTAATGCTGCCCGATGGGCAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGCTGCGTATTATCCCGTCCGCTCAGCCTTCATT
GTCGGCGCTACAGGCGCCACCCGTTCTATCGACGCTGCTGGAGAAGCCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAA
GATCCGATCGAATTTATCCATGCCAGCCGACGTTGCCTGATTCAGCAGAGGGAGATTGGTGCGCACAGTGCGTCGTTTGC
GCAGGCGCTACGGGCTGCATTGCGGGAGGATCCCGATGTGATTCTACTGGGAGAATTGCGCGATACGGAGACGATTCGAT
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGCTATCCACGCTGCATACACGTAGCGCATCGCAGGCGGTCGACCGT
TTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AAAATTATTACCCGCCGTGCAAGGTGGGCGAATCGCGCTGTATGAAGTGCTGACTGCGACTGCGGCAGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAACGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

99.704

0.417
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

40.24

98.521

0.396
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364