Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L62_RS15530 Genome accession   NZ_CP077411
Coordinates   3267370..3268350 (-) Length   326 a.a.
NCBI ID   WP_217071808.1    Uniprot ID   -
Organism   Enterobacter asburiae strain FDAARGOS 1432     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3262370..3273350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L62_RS15500 (I6L62_15500) - 3263246..3263743 (+) 498 WP_217071806.1 SprT family zinc-dependent metalloprotease -
  I6L62_RS15505 (I6L62_15505) endA 3263838..3264545 (+) 708 WP_217071807.1 deoxyribonuclease I -
  I6L62_RS15510 (I6L62_15510) rsmE 3264597..3265328 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6L62_RS15515 (I6L62_15515) gshB 3265348..3266295 (+) 948 WP_029739222.1 glutathione synthase -
  I6L62_RS15520 (I6L62_15520) - 3266383..3266943 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  I6L62_RS15525 (I6L62_15525) ruvX 3266943..3267359 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  I6L62_RS15530 (I6L62_15530) pilT 3267370..3268350 (-) 981 WP_217071808.1 type IV pilus twitching motility protein PilT Machinery gene
  I6L62_RS15535 (I6L62_15535) - 3268368..3269069 (+) 702 WP_048979373.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L62_RS15540 (I6L62_15540) - 3269091..3269657 (+) 567 WP_045888331.1 YggT family protein -
  I6L62_RS15545 (I6L62_15545) yggU 3269654..3269950 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  I6L62_RS15550 (I6L62_15550) rdgB 3269954..3270547 (+) 594 WP_217071809.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  I6L62_RS15555 (I6L62_15555) hemW 3270540..3271676 (+) 1137 WP_217071810.1 radical SAM family heme chaperone HemW -
  I6L62_RS15560 (I6L62_15560) - 3271746..3272108 (+) 363 WP_075204767.1 endonuclease domain-containing protein -
  I6L62_RS15565 (I6L62_15565) - 3272170..3272886 (-) 717 WP_033146612.1 DUF2884 domain-containing protein -
  I6L62_RS15570 (I6L62_15570) - 3272943..3273269 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35538.56 Da        Isoelectric Point: 6.9108

>NTDB_id=581401 I6L62_RS15530 WP_217071808.1 3267370..3268350(-) (pilT) [Enterobacter asburiae strain FDAARGOS 1432]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLELAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRNCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=581401 I6L62_RS15530 WP_217071808.1 3267370..3268350(-) (pilT) [Enterobacter asburiae strain FDAARGOS 1432]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACTCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAACTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTATCCAATGACGCCGGGTTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGTCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGTAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACAGCGGCGGCGGCGAATCTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Acinetobacter baumannii D1279779

48.624

100

0.488
  pilT Acinetobacter baumannii strain A118

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

49.062

98.16

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368