Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L53_RS18675 Genome accession   NZ_CP077291
Coordinates   3985373..3986353 (-) Length   326 a.a.
NCBI ID   WP_042324101.1    Uniprot ID   A0A8H9TWU6
Organism   Citrobacter farmeri strain FDAARGOS 1423     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3980373..3991353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L53_RS18645 (I6L53_18645) - 3981119..3981619 (+) 501 WP_042324090.1 SprT family zinc-dependent metalloprotease -
  I6L53_RS18650 (I6L53_18650) endA 3981711..3982418 (+) 708 WP_042324092.1 deoxyribonuclease I -
  I6L53_RS18655 (I6L53_18655) rsmE 3982498..3983229 (+) 732 WP_042324094.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6L53_RS18660 (I6L53_18660) gshB 3983242..3984189 (+) 948 WP_042324095.1 glutathione synthase -
  I6L53_RS18665 (I6L53_18665) - 3984380..3984943 (+) 564 WP_042324097.1 YqgE/AlgH family protein -
  I6L53_RS18670 (I6L53_18670) ruvX 3984943..3985359 (+) 417 WP_042324100.1 Holliday junction resolvase RuvX -
  I6L53_RS18675 (I6L53_18675) pilT 3985373..3986353 (-) 981 WP_042324101.1 type IV pilus twitching motility protein PilT Machinery gene
  I6L53_RS18680 (I6L53_18680) - 3986371..3987075 (+) 705 WP_042324102.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L53_RS18685 (I6L53_18685) - 3987094..3987660 (+) 567 WP_042324104.1 YggT family protein -
  I6L53_RS18690 (I6L53_18690) yggU 3987657..3987947 (+) 291 WP_042324106.1 DUF167 family protein YggU -
  I6L53_RS18695 (I6L53_18695) - 3987955..3988548 (+) 594 WP_042324109.1 XTP/dITP diphosphatase -
  I6L53_RS18700 (I6L53_18700) hemW 3988541..3989677 (+) 1137 WP_042324111.1 radical SAM family heme chaperone HemW -
  I6L53_RS18705 (I6L53_18705) ansB 3989717..3990763 (-) 1047 WP_042324112.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35446.59 Da        Isoelectric Point: 6.8261

>NTDB_id=580592 I6L53_RS18675 WP_042324101.1 3985373..3986353(-) (pilT) [Citrobacter farmeri strain FDAARGOS 1423]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMECAPFTAADITGLLADWLDEGQQAALQQNGQLDFAVSLVGNLRLRA
SAFHQRQGTSLALRLLPTKCPTLETLGTPSVLPELLSSENGLILVTGATGSGKSTTLAAMVTWLNQHVDGHILTLEDPIE
YVYASQRCLIQQREVGLHCASFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLEADKQEGRVALFELLINTPAAGNLIREGKSHQLPHIIQTGQQVGMMTFAQSLQQR
QAQGKL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=580592 I6L53_RS18675 WP_042324101.1 3985373..3986353(-) (pilT) [Citrobacter farmeri strain FDAARGOS 1423]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCGCG
CTGGCGAAAACGGGGGCGTATGGAATGCGCCCCTTTCACCGCAGCCGACATTACCGGACTGCTCGCGGATTGGCTGGATG
AAGGGCAGCAGGCGGCGTTACAGCAAAATGGACAACTGGACTTCGCCGTTTCGCTTGTGGGAAACCTGCGGTTGCGGGCC
AGCGCATTTCACCAGCGACAGGGAACGTCGCTGGCACTGCGACTGCTGCCGACGAAGTGCCCGACGCTGGAAACACTGGG
GACACCCTCCGTGCTGCCTGAACTGCTCAGCAGCGAGAACGGCTTAATTCTGGTGACGGGCGCCACGGGCAGTGGAAAAT
CGACTACCCTGGCGGCGATGGTGACGTGGCTGAATCAGCATGTTGATGGGCACATTCTGACGCTCGAAGATCCCATCGAA
TATGTCTACGCCAGCCAGCGTTGTCTGATTCAACAGCGTGAGGTGGGATTACACTGCGCTTCGTTTGCCGCAGGGTTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGGGAGCTTCGTGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGTGGCGCTGCGCAGGCGATCGAGCGGTTGGTTGATTCT
TTCCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCTGGCAGTCTGCGTGCCGTGCTGTCGCAAAAGCTGGAAGC
TGACAAGCAGGAGGGACGCGTTGCGCTGTTTGAACTTTTGATTAACACCCCGGCTGCGGGGAATTTAATTCGGGAAGGAA
AGTCCCACCAGTTGCCCCATATCATTCAGACCGGGCAGCAGGTGGGGATGATGACGTTTGCCCAGAGCCTGCAACAGCGA
CAGGCGCAGGGAAAGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

47.095

100

0.472
  pilT Legionella pneumophila strain ERS1305867

46.584

98.773

0.46
  pilT Legionella pneumophila strain Lp02

46.584

98.773

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Vibrio cholerae strain A1552

43.871

95.092

0.417
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383