Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L54_RS05695 Genome accession   NZ_CP077262
Coordinates   1163849..1164829 (+) Length   326 a.a.
NCBI ID   WP_040232091.1    Uniprot ID   A0AAU7ICT9
Organism   Citrobacter pasteurii strain FDAARGOS 1424     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1158849..1169829
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L54_RS05665 (I6L54_05665) ansB 1159364..1160410 (+) 1047 WP_040232083.1 L-asparaginase 2 -
  I6L54_RS05670 (I6L54_05670) hemW 1160525..1161661 (-) 1137 WP_040232085.1 radical SAM family heme chaperone HemW -
  I6L54_RS05675 (I6L54_05675) - 1161654..1162247 (-) 594 WP_005123283.1 XTP/dITP diphosphatase -
  I6L54_RS05680 (I6L54_05680) yggU 1162255..1162545 (-) 291 WP_040232087.1 DUF167 family protein YggU -
  I6L54_RS05685 (I6L54_05685) - 1162542..1163108 (-) 567 WP_003825417.1 YggT family protein -
  I6L54_RS05690 (I6L54_05690) - 1163127..1163831 (-) 705 WP_005123290.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L54_RS05695 (I6L54_05695) pilT 1163849..1164829 (+) 981 WP_040232091.1 type IV pilus twitching motility protein PilT Machinery gene
  I6L54_RS05700 (I6L54_05700) ruvX 1164826..1165242 (-) 417 WP_006686843.1 Holliday junction resolvase RuvX -
  I6L54_RS05705 (I6L54_05705) - 1165242..1165805 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  I6L54_RS05710 (I6L54_05710) gshB 1165981..1166928 (-) 948 WP_005123297.1 glutathione synthase -
  I6L54_RS05715 (I6L54_05715) rsmE 1166948..1167679 (-) 732 WP_040232093.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6L54_RS05720 (I6L54_05720) endA 1167754..1168461 (-) 708 WP_005123302.1 deoxyribonuclease I -
  I6L54_RS05725 (I6L54_05725) - 1168556..1169053 (-) 498 WP_040232095.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36050.30 Da        Isoelectric Point: 6.4971

>NTDB_id=580434 I6L54_RS05695 WP_040232091.1 1163849..1164829(+) (pilT) [Citrobacter pasteurii strain FDAARGOS 1424]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVESAPFTAPDVENLLMCWLSEQQQVQWREQGQIDFAVTLMDSRRLRA
SAFTHQQGMSLALRLLPLECPLLDDLQPPAVIPELLHSENGLILVTGATGSGKSTTLAAMVNYLNQHIEGHILTLEDPIE
YRYTSRRCLIQQREVGVHCTSFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FAASEKDPVRNQLADSLRAVISQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=580434 I6L54_RS05695 WP_040232091.1 1163849..1164829(+) (pilT) [Citrobacter pasteurii strain FDAARGOS 1424]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAAAGTCGAAAGCGCACCGTTTACCGCGCCTGACGTAGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAGCAAGGGCAGATTGATTTTGCCGTTACCCTAATGGACTCCCGACGGCTGCGCGCC
AGCGCATTTACCCATCAACAGGGAATGTCGCTGGCGCTAAGACTGCTACCGCTGGAATGTCCTCTTTTAGACGATCTCCA
GCCTCCCGCGGTCATACCTGAACTGCTGCACAGTGAAAACGGATTGATTCTGGTGACGGGCGCTACCGGCAGCGGTAAAT
CTACGACCCTGGCGGCGATGGTGAACTATCTCAATCAACACATTGAGGGGCATATTCTGACGCTGGAAGATCCTATTGAA
TATCGCTACACCAGCCGACGTTGTCTGATTCAACAGCGGGAGGTGGGCGTACACTGCACCTCTTTTGCCGCCGGTTTGCG
CGGTGCGCTACGCGAAGATCCCGACGTTATTTTGCTGGGCGAGCTGCGCGACGTGGAAACCATTCGGCTGGCATTGACGG
CGGCGGAGACCGGACATCTGGTGCTGGCAACGTTACATACGCGAGGTGCGGCGCAGGCCATCGCGCGGCTGGTAGACACC
TTTGCGGCATCAGAAAAAGATCCCGTGCGTAATCAACTGGCGGATAGCCTGCGGGCGGTTATTTCGCAAAAACTGGAGGA
GGATAAGCAGGGGGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGCGTGATACAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTTTGCAGCAGCGC
CAGGCGCAGGGGCGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Acinetobacter baylyi ADP1

37.461

99.08

0.371